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Sutterella faecalis sp. nov., isolated from human faeces

  • Microbial Systematics and Evolutionary Microbiology
  • Published:
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Abstract

An obligately anaerobic, Gram-stain-negative, non-motile, non-spore-forming, and coccobacilli-shaped bacterial strain, designated KGMB03119T, was isolated from human faeces from a Korean. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the isolate was a member of the genus Sutterella and most closely related to Sutterlla wadsworthensis KCTC 15691T (96.8% 16S rRNA gene sequence similarity). The DNA G + C content of strain KGMB03119T was 58.3 mol% as determined from its whole genome sequence. Strain KGMB03119T was asaccharolytic, catalase-positive, oxidase- and urease-negative. Furthermore, the isolate was positive for alkaline phosphatase, leucine arylamidase, acid phosphatase, arginine arylamidase, alanine arylamidase, and glycine arylamidase. The major cellular fatty acids (> 10%) of the isolate were C18:1ω9c and C16:0. Methylmenaquinone-5 (MMK-5, 100%) was the predominant isoprenoid quinone in the isolate. Based on the phylogenetic, physiological, and chemotaxonomic characteristics, strain KGMB03119T represents a novel species, for which the name Sutterella faecalis sp. nov. is proposed. The type strain is KGMB03119T (= KCTC 15823T = NBRC 114254T).

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Acknowledgments

This work was supported by the Bio & Medical Technology Development program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science (NRF-2016M3A9F3946674) and ICT (MSIT) of the Republic of Korea and a grant from the Korea Research Institute of Bioscience & Biotechnology (KRIBB) Research initiative program. This study was conducted in accordance with the IRB regulation at KCTC (P01-201702-31-007).

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  1. These authors contributed equally to this study

Authors and Affiliations

  1. Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, 56212, Republic of Korea

    Byeong Seob Oh, Ji-Sun Kim, Seung Yeob Yu, Seoung Woo Ryu, Seung-Hwan Park, Se Won Kang, Jam-Eon Park, Seung-Hyeon Choi, Kook-Il Han, Keun Chul Lee, Mi Kyung Eom, Min Kuk Suh, Han Sol Kim, Jung-Sook Lee & Ju Huck Lee

  2. Seoul National University Bundang Hospital, Seongnam, 13620, Republic of Korea

    Dong Ho Lee & Hyuk Yoon

  3. ChunLab Inc., Seoul, 06725, Republic of Korea

    Byung-Yong Kim & Je Hee Lee

  4. University of Science and Technology, Daejeon, 34113, Republic of Korea

    Jung-Sook Lee

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  1. Byeong Seob Oh
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  2. Ji-Sun Kim
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Corresponding authors

Correspondence to Je Hee Lee or Jung-Sook Lee.

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The authors declare that there are no conflicts of interest.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain KGMB03119T is MK999987.

The GenBank/EMBL/DDBJ accession number for the whole genome sequence of strain KGMB03119T is CP040882.

Supplemental material for this article may be found at http://www.springerlink.com/content/120956.

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Oh, B.S., Kim, JS., Yu, S.Y. et al. Sutterella faecalis sp. nov., isolated from human faeces. J Microbiol. 58, 99–104 (2020). https://doi.org/10.1007/s12275-020-9396-9

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