Skip to main content
Log in

Biological and antibacterial properties of TiO2 coatings containing Ca/P/Ag by one-step and two-step methods

  • Published:
Biomedical Microdevices Aims and scope Submit manuscript

Abstract

The porous TiO2 coatings containing Ca/P/Ag were separately prepared on titanium (Ti) surface by one-step (micro-arc oxidation) and two-step methods (micro-arc oxidation and cathodic deposition), and then their surface morphology, composition, biological and antibacterial properties were compared. The results showed that the porous coatings containing Ca/P/Ag achieved by different methods showed similar surface morphology and elemental composition, however, by one-step method, silver existed in the coating as silver phosphate, while in the coatings prepared by two-step method, silver existed as metallic silver. Although both coatings showed excellent antibacterial property (the antimicrobial rate is over 99.9%), the surface coating prepared by one-step method had a more suitable release curve of Ag. In addition, the surface coating prepared by one-step method also presented better biological property, which was due to its enhanced surface roughness and hydrophilicity. Combining with its easy operation and long-term antibacterial property, its prospect for clinical application is more promising.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Y. Abe, T. Hasegawa, M. Kawamura, K. Sasaku, Characterization of Ag oxide thin films prepared by reactive RF sputtering. Vacuum 76, 1–6 (2004)

    Article  Google Scholar 

  • M.R. Bayati, M. Aminzare, R. Molaei, S. Sadrnezhaad, Micro arc oxidation of nano-crystalline Ag-doped TiO2 semiconductors. Mater. Lett. 65, 840–842 (2011)

    Article  Google Scholar 

  • W. Chen, Y. Liu, H.S. Courtney, M. Bettenga, C.M. Agrawal, J.D. Bumgardner, J.L. Ong, In vitro anti-bacterial and biological properties of magnetron co-sputtered silver-containing hydroxyapatite coating. Biomaterials 27, 5512–5517 (2006)

    Article  Google Scholar 

  • H. Cheng, Y. Li, K. Huo, B. Gao, W. Xiong, Long-lasting in vivo and in vitro antibacterial ability of nanostructured titania coating incorporated with silver nanoparticles. J. Biomed. Mater. Res. A 102A, 3488–3499 (2014)

    Article  Google Scholar 

  • L.S. Kibis, V.I. Avdeev, S.V. Koscheev, A.I. Boronin, Oxygen species on the silver surface oxidized by MW-discharge: Study by photoelectron spectroscopy and DFT model calculations. Surf. Sci. 604, 1185–1192 (2010)

    Article  Google Scholar 

  • B. Li, J. Hao, Y. Min, S. Xin, L. Guo, F. He, C. Liang, H. Wang, H. Li. Biological properties of nanostructured Ti incorporated with Ca, P and Ag by electrochemical method. Mat. Sci. Eng. C 51, 80–86 (2015a)

  • B. Li, Y. Li, Y. Min, J. Hao, C. Liang, H. Li, G. Wang, S. Liu, H. Wang, Synergistic effects of hierarchical hybrid micro/nanostructures on the biological properties of titanium orthopaedic implants. RSC Adv. 5, 49552–49558 (2015b)

    Article  Google Scholar 

  • B. Li, J. Li, H. Li, C. Liang, H. Wang, Surface roughness and hydrophilicity of titanium after anodic oxidation. Rare Metal Mat. Eng. 45(4), 857–861 (2016)

    Google Scholar 

  • B. Li, X. Zhang, J. Ma, L. Zhou, H. Li, C. Liang, H. Wang, Hydrophilicity of bioactive titanium surface with different structure, composition, crystal form and grain size. Mater. Lett. 182, 43–46 (2018)

    Article  Google Scholar 

  • S. Liu, B. Li, C. Liang, H. Wang, Z. Qiao, Formation mechanism and adhesive strength of a hydroxyapatite/TiO2 composite coating on a titanium surface prepared by micro-arc oxidation. Appl. Surf. Sci. 362, 109–114 (2016)

    Article  Google Scholar 

  • P.K. Naraharisetti, M.D.N. Lew, Y. Fu, D. Lee, C. Wang, Gentamicin-loaded discs and microspheres and their modifications: Characterization and in vitro release. J. Control. Release 102, 345–359 (2005)

    Article  Google Scholar 

  • Y.K. Pan, C. Chen, D. Wang, Z. Lin, Preparation and bioactivity of micro-arc oxidized calcium phosphate coatings. Mater. Chem. Phys. 141, 842–849 (2013)

    Article  Google Scholar 

  • M. Shokouhfar, S.R. Allahkaram, Formation mechanism and surface characterization of ceramic composite coatings on pure titanium prepared by micro arc oxidation in electrolytes containing nanoparticles. Surf. Coat. Technol. 291, 396–405 (2016)

    Article  Google Scholar 

  • W. Song, H. Ryu, S. Hong, Antibacterial properties of Ag (or Pt)-containing calcium phosphate coatings formed by micro-arc oxidation. J. Biomed. Mater. Res. A 88, 246–254 (2009)

    Article  Google Scholar 

  • J. Sun, S. Huang, X. Guo, Research on the appropriate concentration of silver ion in antibacterial agents. J. Pharm. Res. 36, 516–518 (2017)

    Google Scholar 

  • B. Tian, W. Chen, Y. Dong, J. Marymont, Silver nanoparticle-loaded hydroxyapatite coating: Structure, antibacterial properties, and capacity for osteogenic induction in vitro. RSC Adv. 6, 8549–8562 (2016)

    Article  Google Scholar 

  • Y. Wang, H. Yu, C. Chen, Z. Zhao, Review of the biocompatibility of micro-arc oxidation coated titanium alloys. Mater. Design 85, 640–652 (2015)

    Article  Google Scholar 

  • X. Wang, B. Li, L. Zhou, J. Ma, X. Zhang, H. Li, C. Liang, S. Liu, H. Wang, Influence of surface structures on biocompatibility of TiO2/HA coatings prepared by MAO. Mater. Chem. Phys. 215, 339–345 (2018)

    Article  Google Scholar 

  • B. Zhang, Y. Wang, X. Lu, X. Zhou, Preparation of HA/Ag composite coatings by pulse electrochemical deposition on titanium substrate. Rare Metal Mat. Eng. 39, 1835–1839 (2010)

    Google Scholar 

  • M. Zhao, Application of titanium metal materials and bone tissue engineering in exercise-induced bone injury repair. Adv. Mater. Res. 908, 51–54 (2014)

    Article  Google Scholar 

  • T. Ziebart, B. Al-Nawas, Indication guidelines for bone substitute material. Implantologie 24, 25–31 (2016)

    Google Scholar 

Download references

Acknowledgements

The authors gratefully acknowledge the support by the National Natural Science Foundation of China (Project No.51901239, No.51201056), Natural Science Foundation of Hebei Province of China (Project No.E2017202032, No.E2013202021).

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Baoe Li or Chunyong Liang.

Ethics declarations

Conflict of interest

The authors have declared that no COI exists.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, L., Li, B., Zhang, X. et al. Biological and antibacterial properties of TiO2 coatings containing Ca/P/Ag by one-step and two-step methods. Biomed Microdevices 22, 24 (2020). https://doi.org/10.1007/s10544-020-00482-8

Download citation

  • Published:

  • DOI: https://doi.org/10.1007/s10544-020-00482-8

Keywords

Navigation