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Identification of differentially expressed microRNAs in the skin of experimentally sensitized naturally affected atopic beagles by next-generation sequencing

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Abstract

Canine atopic dermatitis (AD) is a very common inflammatory skin disease, but limited data are available on the genetic characterization (somatic mutations, microarrays, and genome-wide association study (GWAS)) of skin lesions in affected dogs. microRNAs are good biomarkers in inflammatory and neoplastic diseases in people. The aim of this study was to evaluate microRNA expression in the skin of atopic beagles, before and after exposure to Dermatophagoides farinae. Four atopic and four unrelated age-matched healthy beagle dogs were enrolled. Total RNA was extracted from flash-frozen skin biopsies of healthy and atopic dogs. For the atopic dogs, skin biopsies were taken from non-lesional (day 0) and lesional skin (day 28 of weekly environmental challenge with Dermatophagoides farinae). Small RNA libraries were constructed and sequenced. The microRNA sequences were aligned to CanFam3.1 genome. Differential expressed microRNAs were selected on the basis of fold-change and statistical significance (fold-change ≥ 1.5 and p ≤ 0.05 as thresholds. A total of 277 microRNAs were sequenced. One hundred and twenty-one differentially regulated microRNAs were identified between non-lesional and healthy skin. Among these, two were increased amount and 119 were decreased amount. A total of 45 differentially regulated microRNAs between lesional and healthy skin were identified, 44 were decreased amount and one was increased amount. Finally, only two increased amount microRNAs were present in lesional skin when compared with that of non-lesional skin. This is the first study in which dysregulation of microRNAs has been associated with lesional and non-lesional canine AD. Larger studies are needed to understand the role of microRNA in canine AD.

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Author notes

  1. Carmelo Laudanna

    Present address: Stem Cells and Cancer Lab, Institute for Research in Biomedicine Barcelona (IRB), Barcelona, Spain

  2. Rosanna Marsella and Paolo Ciaramella are senior authors on this work. Domenico Santoro and Antonio Di Loria equally contributed to this work.

Authors and Affiliations

  1. Department of Small Animal Clinical Sciences, University of Florida, Gainesville, FL, USA

    Domenico Santoro & Rosanna Marsella

  2. Department of Veterinary Medicine and Animal Productions, University of Naples - Federico II, Naples, Italy

    Antonio Di Loria & Paolo Ciaramella

  3. Department of Experimental and Clinical Medicine, University Magna Graecia of Catanzaro, Catanzaro, Italy

    Teresa Mirante, Duarte Mendes Oliveira, Carmelo Laudanna, Donatella Malanga & Enrico Iaccino

  4. Interdepartmental Center of Services (CIS), University Magna Graecia of Catanzaro, Catanzaro, Italy

    Donatella Malanga

  5. Genetics Unit, IRCCS Istituto Centro San Giovanni di Dio Fatebenefratelli, Brescia, Italy

    Vincenzo Dattilo

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  1. Domenico Santoro
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Correspondence to Domenico Santoro.

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Domenico Santoro and Antonio Di Loria equally contributed to this work.

Electronic supplementary material

ESM 1

Supplemental File S1. DEGS_ mirna.xls (XLS 102 kb)

ESM 2

Supplemental File S2. CTRL_DAY0_IPA_Biofunctions.xls (XLS 49 kb)

ESM 3

Supplemental File S3. CTRL_DAY28_IPA_Biofunctions.xls (XLS 36 kb)

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Supplemental File S4 Network analysis (XLS 44 kb)

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Santoro, D., Di Loria, A., Mirante, T. et al. Identification of differentially expressed microRNAs in the skin of experimentally sensitized naturally affected atopic beagles by next-generation sequencing. Immunogenetics 72, 241–250 (2020). https://doi.org/10.1007/s00251-020-01162-w

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  • DOI: https://doi.org/10.1007/s00251-020-01162-w

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