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Decellularized Cell Culture ECMs Act as Cell Differentiation Inducers

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Abstract

Decellularized tissues and organs have aroused considerable interest for developing functional bio-scaffolds as natural templates in tissue engineering applications. More recently, the use of natural extracellular matrix (ECM) extracted from the in vitro cell cultures for cellular applications have come into question. It is well known that the microenvironment largely defines cellular properties. Thus, we have anticipated that the ECMs of the cells with different potency levels should likely possess different effects on cell cultures. To test this, we have comparatively evaluated the differentiative effects of ECMs derived from the cultures of human somatic dermal fibroblasts, human multipotent bone marrow mesenchymal stem cells, and human induced pluripotent stem cells on somatic dermal fibroblasts. Although challenges remain, the data suggest that the use of cell culture-based extracellular matrices perhaps may be considered as an alternative approach for the differentiation of even somatic cells into other cell types.

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Funding

This work was supported by The Scientific and Technological Research Council of Turkey, Ankara, Turkey (Grant No. 214 M159).

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  1. Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science, and Ankara University Stem Cell Institute, Ankara, Turkey

    Mahmut Parmaksiz, Ayşe Eser Elçin & Yaşar Murat Elçin

  2. Biovalda Health Technologies, Inc, Ankara, Turkey

    Yaşar Murat Elçin

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  1. Mahmut Parmaksiz
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Correspondence to Yaşar Murat Elçin.

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Y.M.E. is the founder and shareholder of Biovalda Health Technologies, Inc. (Ankara, Turkey). The authors have patent applications in relation to regenerative biomaterials. The authors declare no competing financial interests in relation to this particular article.

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Parmaksiz, M., Elçin, A.E. & Elçin, Y.M. Decellularized Cell Culture ECMs Act as Cell Differentiation Inducers. Stem Cell Rev and Rep 16, 569–584 (2020). https://doi.org/10.1007/s12015-020-09963-y

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