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Elucidation of the K32 Capsular Polysaccharide Structure and Characterization of the KL32 Gene Cluster of Acinetobacter baumannii LUH5549

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Abstract

Capsular polysaccharide (CPS), isolated from Acinetobacter baumannii LUH5549 carrying the KL32 capsule biosynthesis gene cluster, was studied by sugar analysis, Smith degradation, and one- and two-dimensional 1H and 13C NMR spectroscopy. The K32 CPS was found to be composed of branched pentasaccharide repeats (K units) containing two residues of β-D-GalpNAc and one residue of β-D-GlcpA (β-D-glucuronic acid) in the main chain and one residue each of β-D-Glcp and α-D-GlcpNAc in the disaccharide side chain. Consistent with the established CPS structure, the KL32 gene cluster includes genes for a UDP-glucose 6-dehydrogenase (Ugd3) responsible for D-GlcA synthesis and four glyco- syltransferases that were assigned to specific linkages. Genes encoding an acetyltransferase and an unknown protein prod- uct were not involved in CPS biosynthesis. Whilst the KL32 gene cluster has previously been found in the global clone 2 (GC2) lineage, LUH5549 belongs to the sequence type ST354, thus demonstrating horizontal gene transfer between these lineages.

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Abbreviations

CPS:

capsular polysaccharide

KL:

chromosomal K locus

K unit:

oligosaccharide repeat

MPS:

modified polysaccharide

PSgc:

polysaccharide gene cluster

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Funding

This work was supported by the Russian Science Foundation (project no. 19-14-00273) to YAK and Australian Research Council (ARC) DECRA Fellowship 180101563 to JJK.

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Author notes

  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, QLD 4001, Brisbane, Australia

    S. M. Cahill & J. J. Kenyon

  2. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russia

    N. P. Arbatsky, A. S. Shashkov & Y. A. Knirel

  3. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia

    M. M. Shneider

  4. Moscow Institute of Physics and Technology, 141701, Dolgoprudny, Moscow Region, Russia

    A. V. Popova

  5. State Research Center for Applied Microbiology and Biotechnology, 142279, Obolensk, Moscow Region, Russia

    A. V. Popova

  6. School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia

    R. M. Hall

Authors
  1. S. M. Cahill
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  2. N. P. Arbatsky
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  3. A. S. Shashkov
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  6. R. M. Hall
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  7. J. J. Kenyon
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  8. Y. A. Knirel
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Corresponding author

Correspondence to Y. A. Knirel.

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Ethical approval. This article does not contain any studies with human participants or animals performed by any of the authors.

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Conflict of interest. The authors declare no conflict of interest in financial nor in any other area.

Published in Russian in Biokhimiya, 2020, Vol. 85, No. 2, pp. 280-286.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM19-307, December 30, 2019.

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Cahill, S.M., Arbatsky, N.P., Shashkov, A.S. et al. Elucidation of the K32 Capsular Polysaccharide Structure and Characterization of the KL32 Gene Cluster of Acinetobacter baumannii LUH5549. Biochemistry Moscow 85, 241–247 (2020). https://doi.org/10.1134/S000629792002011X

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