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Complete genome sequence of a novel bovine hepacivirus from Yunnan, China

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Abstract

We detected a novel bovine hepacivirus N (HNV) subtype, IME_BovHep_01, in the serum of cattle in Tengchong, Yunnan, China, by high-throughput sequencing. The complete genome of IME_BovHep_01, was sequenced using an Illumina MiSeq sequencer and found to be 8850 nt in length, encoding one hypothetical protein. BLASTn analysis showed that the genome sequence shared similarity with the bovine hepacivirus isolate BovHepV_209/Ger/2014, with 88% query coverage and 70.8% identity. However, the highest similarity was to bovine hepacivirus N strain BRBovHep_RS963, for which only a partial genome sequence is available, with 68% query coverage and 81.5% identity. Sequence comparisons and phylogenetic analysis suggested that IME_BovHep_01 is a novel HNV subtype. Importantly, IME_BovHep_01 is the first member of this new genotype for which the complete genome sequence was determined.

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Acknowledgements

The authors would like to thank Guangqian Pei, Wenjuan Li, Yawei Zhang, Mingming Gao, and Wei Lin for their help during this work.

Funding

This research was supported by grants from the National Science and Technology Major Project (2018ZX10201001), the China Mega-Project on Infectious Disease Prevention (2018ZX10305410-001, 2018ZX10711001-003-001), and the National Natural Science Foundation of China (81760607).

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Author notes

  1. Xin Qiang, Xueying Shen, Honghong Peng and Xiaofang Guo contribute equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, People’s Republic of China

    Xin Qiang, Honghong Peng, Yujun Cui, Xianglilan Zhang, Yong Huang, Hang Fan, Yigang Tong & Zhiqiang Mi

  2. Tengchong Center for Animal Disease Control and Prevention, Tengchong, 679100, Yunnan, People’s Republic of China

    Xueying Shen & Guozhang Fu

  3. Anhui Medical University, Hefei, 230000, People’s Republic of China

    Honghong Peng

  4. Yunnan Provincial Key Laboratory of Vector-borne Diseases Control and Research, Yunnan Institute of Parasitic Diseases, Pu’er, 665000, Yunnan, People’s Republic of China

    Xiaofang Guo

  5. Yunnan Institute for Endemic Diseases Control and Prevention, Dali, 671000, Yunnan, People’s Republic of China

    Zhihai He, Mingguo Yao & Chunhong Du

  6. Beijing Advanced Innovation Center for Soft Matter Science and Engineering (BAIC-SM), College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People’s Republic of China

    Yigang Tong

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  1. Xin Qiang
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Correspondence to Hang Fan, Chunhong Du, Yigang Tong or Zhiqiang Mi.

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Qiang, X., Shen, X., Peng, H. et al. Complete genome sequence of a novel bovine hepacivirus from Yunnan, China. Arch Virol 165, 1489–1494 (2020). https://doi.org/10.1007/s00705-020-04611-9

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  • DOI: https://doi.org/10.1007/s00705-020-04611-9

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