A quantum thermometric sensing and analysis system using fluorescent nanodiamonds for the evaluation of living stem cell functions according to intracellular temperature

Hiroshi Yukawa; Masazumi Fujiwara; Kaori Kobayashi; Yuka Kumon; Kazu Miyaji; Yushi Nishimura; Keisuke Oshimi; Yumi Umehara; Yoshio Teki; Takayuki Iwasaki; Mutsuko Hatano; Hideki Hashimoto; Yoshinobu Baba

doi:10.1039/D0NA00146E

A quantum thermometric sensing and analysis system using fluorescent nanodiamonds for the evaluation of living stem cell functions according to intracellular temperature†

Hiroshi Yukawa,

‡*^abc Masazumi Fujiwara,

‡*^d Kaori Kobayashi,^b Yuka Kumon,^b Kazu Miyaji,^b Yushi Nishimura,^cd Keisuke Oshimi,^d Yumi Umehara,^d Yoshio Teki,

^d Takayuki Iwasaki,^e Mutsuko Hatano,^e Hideki Hashimoto^f and Yoshinobu Baba*^abc

Author affiliations

* Corresponding authors

^a Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University, Nagoya, Japan
E-mail: h.yukawa@nanobio.nagoya-u.ac.jp, babaymtt@chembio.nagoya-u.ac.jp

^b Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Japan

^c Institute of Quantum Life Science, National Institutes for Quantum and Radiological Science and Technology, Chiba, Japan

^d Department of Chemistry, Graduate School of Science, Osaka City University, Osaka, Japan
E-mail: masazumi@osaka-cu.ac.jp

^e Department of Electrical and Electronic Engineering, Graduate School of Engineering, Tokyo Institute of Technology, Tokyo, Japan

^f School of Science and Technology, Kwansei Gakuin University, Hyogo, Japan

Abstract

Intracellular thermometry techniques play an important role in elucidating the relationship between the intracellular temperature and stem cell functions. However, there have been few reports on thermometry techniques that can detect the intracellular temperature of cells during several days of incubation. In this study, we developed a novel quantum thermometric sensing and analysis system (QTAS) using fluorescent nanodiamonds (FNDs). FNDs could label adipose tissue-derived stem cells (ASCs) at high efficiency with 24 h of incubation, and no cytotoxicity was observed in ASCs labeled with less than 500 μg mL⁻¹ of FNDs. The peak of FNDs was confirmed at approximately 2.87 GHz with the characteristic fluorescence spectra of NV centers that could be optically detected (optically detected magnetic resonance [ODMR]). The ODMR peak clearly shifted to the high-frequency side as the temperature decreased and gave a mean temperature dependence of −(77.6 ± 11.0) kHz °C⁻¹, thus the intracellular temperature of living ASCs during several days of culturing could be precisely measured using the QTAS. Moreover, the intracellular temperature was found to influence the production of growth factors and the degree of differentiation into adipocytes and osteocytes. These data suggest that the QTAS can be used to investigate the relationship between intracellular temperature and cellular functions.

Nanoscale Advances

A quantum thermometric sensing and analysis system using fluorescent nanodiamonds for the evaluation of living stem cell functions according to intracellular temperature†

Abstract

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A quantum thermometric sensing and analysis system using fluorescent nanodiamonds for the evaluation of living stem cell functions according to intracellular temperature

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