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Physiological and proteomic responses of mangrove plant Avicennia marina seedlings to simulated periodical inundation

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Abstract

Background and aims

Avicennia marina is a pioneer mangrove species widely distributed along the southeast coast of China. It suffers, especially in the seedling stage, from the tidal flooding and anaerobic surroundings as it closes to the seaward side. The aim of this study is to assess the ability of A. marina seedlings to withstand different tidal inundations by physiological and proteomic approaches.

Methods

To accomplish this, the artificial tidal inundations were mimicked as 0, 4, and 8 h per tidal cycle about 1 week. The physiological approaches and two-dimensional electrophoresis coupled with MALDI-TOF/TOF-MS technology were used to reveal the adaptive alterations of A. marina seedlings to tidal inundation.

Results

There were profound changes in photosynthesis and chlorophyll fluorescence characteristics of A. marina seedling leaves under inundation treatment. Particularly, photosynthetic rate increased at 4 h inundation, while decreasing at 8 h inundation treatment compared with control (0 h). The results highlighted that A. marina seedlings could greatly upregulate the abundances of photosynthesis-related proteins, activate antioxidant and defense systems to efficiently suppress the reactive oxygen species (ROS) burst and cell damages, mobilize carbohydrate metabolism associated proteins, and then maintain cellular homeostasis, resulting in well adaptation to short-term tidal inundation.

Conclusion

Overall, our results demonstrated that short-term inundation is beneficial, while long-term inundation is detrimental to A. marina. The results allow us to recognize the roles of specific proteins in molecular tolerance to tidal inundation in mangrove plants.

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Acknowledgments

We appreciate anonymous reviewers and editor for the insightful comments and valuable suggestions.

Funding

This work was financially supported by the National Key Research and Development Program of China (2017YFC0506102) and the Natural Science Foundation of China (NSFC) (31870581, 31570586, 31601133, 30930076).

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Author notes

  1. Huan Li, Zan Li and Zhi-Jun Shen contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, College of the Environment and Ecology, Xiamen University, Xiamen, 361005, Fujian, People’s Republic of China

    Huan Li, Zan Li, Zhi-Jun Shen, Mei-Rong Luo, Yi-Ling Liu, Ming-Yue Wei, Ying-Ying Qin, Chang-Hao Gao, Ke-Ke Li, Qian-Su Ding, Shan Zhang, Xi-Min Zhang, Gui-Feng Gao, Xue-Yi Zhu & Hai-Lei Zheng

  2. Fujian Key Laboratory of Subtropical Plant Physiology and Biochemistry, Fujian Institute of Subtropical Botany, Xiamen, 361006, Fujian, People’s Republic of China

    Wen-Hua Wang

  3. College of the Environment and Ecology, Xiamen University, Xiamen, 361102, Fujian, People’s Republic of China

    Hai-Lei Zheng

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Contributions

H.L. and H.L.Z.: designed the experiments; H.L. and Z.L.: performed the experiments; H.L. and Z.J.S.: analyzed the proteomic data; H.L.: wrote the paper; Y.L.L., M.Y.W., W.H.W., Y.Y.Q., C.H.G., K.K.L., Q.S.D., S.Z., X.M.Z., G.F.G., and X.Y.Z.: gave the suggestions; H.L.Z: revised this paper. All authors have read and approved the manuscript.

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Correspondence to Hai-Lei Zheng.

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Supplementary Fig. S1

Sampling site in the National Nature Reserve for Mangroves in Zhangjiang Estuary, Fujian, China and the laboratory simulated periodical inundation site in Xiamen University, Fujian, China. The white spot in the image represents the sampling site for Avicennia marina. (PNG 505 kb)

High Resolution Image (TIF 1340 kb)

Supplementary Fig. S2

The monthly average temperature (A) and the monthly average relative air humidity (B) in Yunxiao and Xiamen were obtained according to the data of The National Marine Data and Information Service (NMDIS), 1981-2010. The diurnal variations of tidal level of sample site (Yunxiao) in 15-Sep-2018 (C). (PNG 258 kb)

High Resolution Image (TIFF 1692 kb)

Supplementary Fig. S3

Example of inundation system and culture tank used in the present study. There are 3 (inundation times) × 3 (replicates) = 9 sets of such culture systems. And each culture pot contains four individual seedlings. (PNG 122 kb)

High Resolution Image (TIFF 1581 kb)

Supplementary Fig. S4

2-DE analysis of proteins extracted from the leaves of A. marina seedlings under periodical inundations. The numbers assigned to the protein spots correspond to those listed in Table 2. (A) representative CBB-R250 stained 2D gel of total proteins extracted from 4 h inundation-treated leaves; (B) the enlarged windows of representative protein spots with different expressions under 0 h, 4 h and 8 h inundation treatment. (PNG 631 kb)

High Resolution Image (TIF 7333 kb)

Supplementary Fig. S5

(A) Functional category distribution of the identified proteins in A. marina leaves, (B) protein subcellular localizations of all identified and quantified proteins. (PNG 221 kb)

High Resolution Image (TIF 1363 kb)

Supplementary Fig. S6

The protein–protein interaction (PPI) network analysis in the leaves of A. marina under different treatments. (A) The PPI network under 4 h inundation treatment, (B) the PPI network under 8 h inundation treatment. Nodes in red color represent up-regulated, and green color represents down-regulated in protein abundance. (PNG 1027 kb)

High Resolution Image (TIF 9585 kb)

Supplementary Table S1

The detailed information of the peptide sequence, expected and observed m/z values for the differentially expressed proteins identified by MALDI-TOF/TOF MS in the leaves of A. marina under different inundation treatments. (DOCX 34 kb)

Supplementary Table S2

Information of clusters generated by MCODE plugin. (DOCX 13 kb)

Supplementary Table S3

The detailed information of interaction network among the differentially expressed proteins generated by String 10.5. (DOCX 39 kb)

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Li, H., Li, Z., Shen, ZJ. et al. Physiological and proteomic responses of mangrove plant Avicennia marina seedlings to simulated periodical inundation. Plant Soil 450, 231–254 (2020). https://doi.org/10.1007/s11104-020-04474-8

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  • DOI: https://doi.org/10.1007/s11104-020-04474-8

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