Issue 22, 2020
From the journal:

RSC Advances

Bead based facile assay for sensitive quantification of native state green fluorescent protein†

Jung Min Kim,a   Baik Lin Seong*b  and  Dong-Kwon Lim ORCID logo *a  

* Corresponding authors

a KU-KIST Graduate School of Converging Science and Technology, Korea University 145 Korea-ro, Seongbuk-gu, Seoul, Republic of Korea
E-mail: dklim@korea.ac.kr

b Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, 50, Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea
E-mail: blseong@yonsei.ac.kr

Abstract

A facile method for the quantification of native state protein is strongly required to accurately determine the amount of expressed protein of interest. Here we report a simple bead-based assay, which can sensitively quantify the amount of native state green fluorescent protein using Ni-NTA (nickel-nitrilotriacetic acid)-modified microbead particles. The bead-based method is simple and straightforward to perform and it showed a highly sensitive capability to detect the expressed fluorescent protein because of the enriched fluorescent protein on the beads.

Graphical abstract: Bead based facile assay for sensitive quantification of native state green fluorescent protein

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Article information

DOI
https://doi.org/10.1039/C9RA09599C
Article type
Paper
Submitted
18 Nov 2019
Accepted
20 Mar 2020
First published
01 Apr 2020
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2020,10, 13095-13099

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Bead based facile assay for sensitive quantification of native state green fluorescent protein

J. M. Kim, B. L. Seong and D. Lim, RSC Adv., 2020, 10, 13095 DOI: 10.1039/C9RA09599C

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