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Interspecific hybridization between cultivated morels Morchella importuna and Morchella sextelata by PEG-induced double inactivated protoplast fusion

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Abstract

The commercial production of Morchella mushrooms calls for urgent breeding of excellent varieties or strains with appropriate tools, such as protoplast fusion. However, the protoplast fusion in morels has not been studied. In this paper, interspecific hybridization between cultivated morels of M. importuna and M. sextelata by PEG-induced protoplast fusion was conducted. Apart from functional complementation of double inactivated protoplasts, the fusants were characterized by cultural and cultivated characters and molecular markers of random amplified polymorphic DNA (RAPD). The results suggested that the hybrids and their parents showed significant difference in their inoculum recovery time, mycelial growth rate, yield of cultivation and total amino acid content of ascocarps. Moreover, positive barrage reactions were observed between parental strains as well as between each parent and a hybrid line. A dendrogram created on the basis of RAPD fingerprints exhibited three major clusters, in which morel hybrids showed intra-cluster variations, M. sextelata #6 formed an out group, while M. importuna #4 was phylogenetically closer to morel hybrids. All the results demonstrated that real fusants were obtained in our study. Protoplast fusion may provide an ideal alternative for new strain selection, and thus will promote the healthy development of morel industry.

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Abbreviations

ANOVA:

Analysis of variance

CYM:

Complete yeast extracts medium

GB:

Guo Biao (Chinese National Standard)

PEG:

Polyethylene glycol

RAPD:

Random amplified polymorphic DNA

RCYM:

CYM supplemented with 0.6 M mannitol

SD:

Standard deviation

UPGMA:

Unweighted pair-group method with mathematic averages

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Authors and Affiliations

  1. School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450002, China

    Peixin He, Miao Yu & Ke Wang

  2. Institute of Vegetable, Wuhan Academy of Agricultural Sciences, Wuhan, 430070, China

    Yingli Cai

  3. Zhengzhou Institute of Agriculture and Forestry, Zhengzhou, 450005, China

    Bin Li

  4. Institute of Applied Mycology, Huazhong Agricultural University, Wuhan, 430070, China

    Wei Liu

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  1. Peixin He
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  2. Miao Yu
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  3. Ke Wang
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  4. Yingli Cai
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  5. Bin Li
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Correspondence to Wei Liu.

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He, P., Yu, M., Wang, K. et al. Interspecific hybridization between cultivated morels Morchella importuna and Morchella sextelata by PEG-induced double inactivated protoplast fusion. World J Microbiol Biotechnol 36, 58 (2020). https://doi.org/10.1007/s11274-020-02835-0

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