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The efficiency of Cytochrome P450 gene-based markers in accessing genetic variability of drumstick (Moringa oleifera Lam.) accessions

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Abstract

Drumstick (Moringa oleifera Lam.) is an important vegetable as well as forage crop of arid and semi-arid zones of the tropics. The leaves and pods of the plant are rich sources of minerals and vitamins. In the present work, genetic diversity study of 23 genotypes of M. oleifera collected from Kerala, Tamil Nadu and Karnataka states of India was carried out using seven cytochrome P450 (CytP450) markers. By using seven pairs of CytP450 gene-based markers, 88.25% of polymorphism was recorded among the 23 sampled genotypes. The Polymorphic Information Content (PI), Marker Index (MI) and Resolving Power obtained for seven primers were estimated 0.23, 2.96 and 9.83, respectively. The Unweighted Pair Group Method with Arithmetic mean (UPGMA) dendrogram based on this marker data indicate that genotypes from different geographical regions are placed in the same clusters. The dendrogram and Principal Coordinates Analysis (PCoA) plots derived from the binary data matrices were highly concordant. The investigation, in brief, proved that CytP450 based marker system is efficient in the elucidation of genetic diversity in M. oleifera accessions.

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Acknowledgements

The authors are grateful to Dr. Suhara Beevy S, Professor and Head, Department of Botany, University of Kerala for the facilities provided. DRRS wish to thank the University of Kerala for granting research fellowship (No.Ac.EI/A2/10625/2016-I) to undertake the present work.

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  1. Department of Biotechnology, University of Kerala, Kariavattom, Trivandrum, 695581, India

    R. S. Drisya Ravi

  2. Department of Botany, University of Kerala, Kariavattom, Trivandrum, 695581, India

    E. A. Siril & Bindu R. Nair

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  1. R. S. Drisya Ravi
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Ravi, R.S.D., Siril, E.A. & Nair, B.R. The efficiency of Cytochrome P450 gene-based markers in accessing genetic variability of drumstick (Moringa oleifera Lam.) accessions. Mol Biol Rep 47, 2929–2939 (2020). https://doi.org/10.1007/s11033-020-05391-w

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