circ_ARF3 regulates the pathogenesis of osteosarcoma by sponging miR-1299 to maintain CDK6 expression
Section snippets
Background
Osteosarcoma is the most common primary sarcoma of bone which occurred predominantly in children and adolescence [1,2]. Recent years advancement in non-metastatic osteosarcoma treatment, including surgical resection, neoadjuvant and adjuvant combinational chemotherapy have improved the 5-year survival rate approaches 70% [3]. However, for the metastatic or relapsed osteosarcoma the prognosis remains poor and the 5 year survival rates is less than 20%, with no improvement over the past 20 years [4
Cell lines and antibodies
Osteosarcoma cell lines Saos2, U2OS and MG-63 were purchased from ATCC. Normal osteoblast cell line NHOst was purchased from Chinese Academy of Sciences, Shanghai china. Saos2 and U2OS were cultured in McCoy's 5a Medium supplemented with 10% FBS (Gibco), MG63 was grown in Eagle's Minimum Essential Medium (EMEM) supplemented with 10% FBS (Gibco). NHOst was cultured in DMEM with 10% FBS added. All the cell lines were subjected to incubation in a humidified environment with 5% CO2 at 37 °C.
Characterize of circ_ARF3 expression in OS cell lines
Previous genome wide transcriptome sequencing in lung cancer showed that circ_ARF3 was highly expressed in lung cancer tissues than in normal adjacent tissues. However, the roles and expression of circ_ARF3 in OS are still unknown. To determine if circ_ARF3 also expresses in OS, we first checked the circular RNA database from circinteractome, results showed that circ_ARF3 is back spliced by exon 3,4 and 5 of ARF3 transcripts (Fig. 1A). To further confirm the circular form of ARF3 transcript is
Discussion
CircRNAs are recently discovered noncoding RNAs characterized by covalently closed loop structures with no polyadenylated tail [18]. Most circRNAs are generated through pre-mRNA back-splicing with upstream 3′ splice site connected to the downstream 5'splice site [19]. Recent studies have shown that circRNAs play important roles in multiple biological process, including carcinogenesis, and were associated with many cancer progressions, for example, in liver cancer, circ_100395 was found
Declaration of Competing Interest
The authors declare that they have no competing interests.
Acknowledgements
This project was supported by The China Scholarship Council.
Credit author statement
Gao Ai-Mei: Conceptualization, writing first draft, methodology and data analysis. Yuan Chunyan: Data curation and validation. Ai-Xin Hu: Western blots and in vivo assay. Liu Xiang-Sheng supervision, writing-review and editing.
References (36)
Primary bone osteosarcoma in the pediatric age: state of the art
Cancer Treat. Rev.
(2006)Global identification of circular RNAs in chronic myeloid leukemia reveals hsa_circ_0080145 regulates cell proliferation by sponging miR-29b
Biochem. Biophys. Res. Commun.
(2018)circRNA biogenesis competes with pre-mRNA splicing
Mol. Cell
(2014)A long noncoding RNA controls muscle differentiation by functioning as a competing endogenous RNA
Cell
(2011)Osteogenic sarcoma
Orthop. Nurs.
(2006)Osteosarcoma: current treatment and a collaborative pathway to success
J. Clin. Oncol.
(2015)Biology of childhood osteogenic sarcoma and potential targets for therapeutic development: meeting summary
Clin. Cancer Res.
(2003)Genome-wide identification of circular RNAs in fungal parasite Nosema ceranae
Curr. Microbiol.
(2018)Circ-ITGA7 sponges miR-3187-3p to upregulate ASXL1, suppressing colorectal cancer proliferation
Cancer Manag. Res.
(2019)- et al.
Past, present, and future of circRNAs
EMBO J.
(2019)
Circular RNAs are long-lived and display only minimal early alterations in response to a growth factor
Nucleic Acids Res.
CircHIPK3 sponges miR-558 to suppress heparanase expression in bladder cancer cells
EMBO Rep.
Loss of a mammalian circular RNA locus causes miRNA deregulation and affects brain function
Science
Long noncoding and circular RNAs in lung cancer: advances and perspectives
Epigenomics
Noncoding RNAs in gastric cancer: research progress and prospects
World J. Gastroenterol.
Circular RNA in liver: health and diseases
Adv. Exp. Med. Biol.
Roles of circular RNAs in neurologic disease
Front. Mol. Neurosci.
Circular RNA Signature in Hepatocellular Carcinoma
J. Cancer
Cited by (12)
Circular RNA and human diseases: Basic research and translational implications
2021, Cellular SignallingLung cancer cells and their sensitivity/resistance to cisplatin chemotherapy: Role of microRNAs and upstream mediators
2021, Cellular SignallingCitation Excerpt :So, an alteration in expression of miRs is related to emergence of pathological conditions, particularly cancer [78–83]. Notably, in respect to different and abnormal expressions of miRs in cancer, they can be employed as diagnostic and prognostic factors in cancer [84–86]. More importantly, it has been demonstrated that miRs can contribute to resistance of cancer cells to chemotherapy and much fascination has been directed towards this field [87–89].
CircARF3 Mitigates Allergic Rhinitis through Targeting microRNA-205-5p/Sirtuin 5 Axis
2023, International Archives of Allergy and ImmunologyMiR-1299 is regulated by KCNQ1OT1 and inhibits cervical cancer progression
2023, Cellular and Molecular BiologyA Novel Circ_Arf3/miR-452-5p/Mbnl1 Axis Regulates Proliferation and Expression of Fibrosis-Related Proteins of Mouse Mesangial Cells Under High Glucose
2023, Diabetes, Metabolic Syndrome and ObesityCirc_0136666 aggravates osteosarcoma development through mediating miR-1244/CEP55 axis
2022, Journal of Orthopaedic Surgery and Research
- 1
Co-first authors