Biotin and glucose dual-targeting, ligand-modified liposomes promote breast tumor-specific drug delivery

https://doi.org/10.1016/j.bmcl.2020.127151Get rights and content

Abstract

Breast cancer is the second leading cause of cancer-related deaths in women. Ligand-modified liposomes are used for breast tumor-specific drug delivery to improve the efficacy and reduce the side effects of chemotherapy; however, only a few liposomes with high targeting efficiency have been developed because the mono-targeting, ligand-modified liposomes are generally unable to deliver an adequate therapeutic dose. In this study, we designed biotin-glucose branched ligand-modified, dual-targeting liposomes (Bio-Glu-Lip) and evaluated their potential as a targeted chemotherapy delivery system in vitro and in vivo. When compared with the non-targeting liposome (Lip), Bio-Lip, and Glu-Lip, Bio-Glu-Lip had the highest cell uptake in 4T1 cells (3.00-fold, 1.60-fold, and 1.95-fold higher, respectively) and in MCF-7 cells (2.63-fold, 1.63-fold, and 1.85-fold higher, respectively). The subsequent cytotoxicity and in vivo assays further supported the dual-targeting liposome is a promising drug delivery carrier for the treatment of breast cancer.

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Declaration of Competing Interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 81573286, No. 81773577, No. 21472130, and No. 81803420), the Sichuan Science and Technology Program (2018JY0537).

References (26)

  • M. Akram et al.

    Awareness and current knowledge of breast cancer

    Biol Res

    (2017)
  • C.I. Li

    Breast Cancer Epidemiol

    (2010)
  • R. Mahato et al.

    Prodrugs for improving tumor targetability and efficiency

    Adv Drug Deliv Rev

    (2011)
  • A. Shafei et al.

    A review on the efficacy and toxicity of different doxorubicin nanoparticles for targeted therapy in metastatic breast cancer

    Biomed Pharmacother

    (2017)
  • A. Satsangi et al.

    Synthesis of a novel, sequentially active-targeted drug delivery nanoplatform for breast cancer therapy

    Biomaterials

    (2015)
  • J. Rip et al.

    Glutathione PEGylated liposomes: pharmacokinetics and delivery of cargo across the blood-brain barrier in rats

    J Drug Target

    (2014)
  • S. Saha et al.

    Biotin-conjugated tumour-targeting photocytotoxic iron(III) complexes

    Philos Trans R Soc A Math Phys Eng Sci

    (2013)
  • R.A. Cairns et al.

    Regulation of cancer cell metabolism

    Nat Rev Cancer

    (2011)
  • P.P. Hsu et al.

    Cancer cell metabolism: Warburg and beyond

    Cell

    (2008)
  • Ł. Uram et al.

    Cellular uptake of glucoheptoamidated poly(amidoamine) PAMAM G3 dendrimer with amide-conjugated biotin, a potential carrier of anticancer drugs

    Bioorganic Med Chem

    (2017)
  • W.X. Ren et al.

    Recent development of biotin conjugation in biological imaging, sensing, and target delivery

    Chem Commun

    (2015)
  • O. Minenkova et al.

    Growth inhibition of human ovarian carcinoma by a novel AvidinOX-anchored biotinylated camptothecin derivative

    Bioorg Med Chem Lett

    (2018)
  • C. Granchi et al.

    An update on therapeutic opportunities offered by cancer glycolytic metabolism

    Bioorg Med Chem Lett

    (2014)
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