Abstract
The non-destructive method for detection of fungal contamination in peach fruit using hyperspectral imaging was evaluated. Growth characteristics of three major spoilage fungi in peach fruit during decay were estimated. Three quantitative prediction models were then constructed to forecast the microbial content from the HSI datasets. The prediction of fungal contamination on the fruit was visualized with different colors. Additionally, principal component analysis (PCA) was applied to reduce the dimensionality of the HSI data and to discriminate the infection degree in peaches. The results showed that partial least squares regression (PLSR) could achieve performance with Rp2 not less than 0.84in predicting fungal colony counts, while PCA scores successfully identified the infected degrees of samples. This study illustrates that HSI combined with chemometrics can potentially be implemented for the quantitative detection of fungal contamination in peach fruit.
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References
Ariana DP, Lu R (2010) Hyperspectral waveband selection for internal defect detection of pickling cucumbers and whole pickles. Comput Electron Agric 74(1):137–144. https://doi.org/10.1016/j.compag.2010.07.008
Casals C, Teixidó N, Viñas I, Cambray J, Usall J (2010) Control of Monilinia spp. on stone fruit by curing treatments. Part II: The effect of host and Monilinia spp. variables on curing efficacy. Postharvest Biol Technol 56(1):26–30. https://doi.org/10.1016/j.postharvbio.2009.11.009
Chen L, Li Z, Yu F, Zhang X, Xue Y, Xue C (2019) Hyperspectral imaging and chemometrics for nondestructive quantification of total volatile basic nitrogen in pacific oysters (Crassostrea gigas). Food Anal Methods 3(12):799–810
Galvao RKH, Araujo MCU, José GE, Pontes MJC, Silva EC, Saldanha TCB (2005) A method for calibration and validation subset partitioning. Talanta 67(4):736–740. https://doi.org/10.1016/j.talanta.2005.03.025
GB 4789.15–2016 (2016) National food safety standard food microbiological examination: enumeration of molds and yeasts, China
Guo W, Zhao F, Dong J (2016) Nondestructive measurement of soluble solids content of kiwifruits using near-infrared hyperspectral imaging. Food Anal Methods 9(1):38–47. https://doi.org/10.1007/s12161-015-0165-z
Howard DL, Kjaergaard HG (2006) Influence of intramolecular hydrogen bond strength on OH-stretching overtones. J Phys Chem A 110(34):10245–10250
Huang L, Meng L, Zhu N, Wu D (2017) A primary study on forecasting the days before decay of peach fruit using near-infrared spectroscopy and electronic nose techniques. Postharvest Biol Technol 133. https://doi.org/10.1016/j.postharvbio.2017.07.014
Kamruzzaman M, ElMasry G, Sun DW, Allen P (2012) Prediction of some quality attributes of lamb meat using near-infrared hyperspectral imaging and multivariate analysis. Anal Chim Acta 714:57–67. https://doi.org/10.1016/j.aca.2011.11.037
Kaya-Celiker H, Mallikarjunan PK, Iii DS, Christie ME (2014) Discrimination of moldy peanuts with reference to aflatoxin using FTIR-ATR system. Food Control 44:64–71. https://doi.org/10.1016/j.foodcont.2014.03.045
Kaya-Celiker H, Mallikarjunan PK, Kaaya A (2015) Characterization of invasion of genus Aspergillus on peanut seeds using FTIR-PAS. Food Anal Methods 9(1):105–113. https://doi.org/10.1007/s12161-015-0159-x
Khoshnoudi-Nia S, Moosavi-Nasab M (2019) Food Anal Methods 12:1635. https://doi.org/10.1007/s12161-019-01494-8
Li J, Huang W, Tian X, Wang C, Fan S, Zhao C (2016) Fast detection and visualization of early decay in citrus using Vis-NIR hyperspectral imaging. Comput Electron Agric 127:582–592. https://doi.org/10.1016/j.compag.2016.07.016
Liu D, Pu H, Sun DW, Wang L, Zeng XA (2014) Combination of spectra and texture data of hyperspectral imaging for prediction of pH in salted meat. Food Chem 160:330–337. https://doi.org/10.1016/j.foodchem.2014.03.096
Liu Q, Zhao N, Zhou D, Sun Y, Sun K, Pan L, Tu K (2018) Discrimination and growth tracking of fungi contamination in peaches using electronic nose. Food Chem 262:226–234. https://doi.org/10.1016/j.foodchem.2018.04.100
Liu Q, Wei K, Xiao H, Tu S, Sun K, Sun Y et al (2019) Near-infrared hyperspectral imaging rapidly detects the decay of postharvest strawberry based on water-soluble sugar analysis. Food Anal Methods 4(12):936–946
Magwaza LS, Opara UL (2015) Analytical methods for determination of sugars and sweetness of horticultural products—a review. Sci Hortic 184:179–192. https://doi.org/10.1016/j.scienta.2015.01.001
Mehl PM, Chen YR, Kim MS, Chan DE (2004) Development of hyperspectral imaging technique for the detection of apple surface defects and contaminations. J Food Eng 61(1):67–81. https://doi.org/10.1016/S0260-8774(03)00188-2
Mireei SA (2010) Nondestructive determination of effective parameters on maturity of mozafati & shahani date fruits by NIR spectroscopy technique. PhD thesis. Iran: Department of Mechanical, Engineering of Agricultural Machinery, University of Tehran. In Persian
Mokrani A, Krisa S, Cluzet S, Da CG, Temsamani H, Renouf E et al (2016) Phenolic contents and bioactive potential of peach fruit extracts. Food Chem 202:212–220. https://doi.org/10.1016/j.foodchem.2015.12.026
Moscetti R, Haff RP, Stella E, Contini M, Monarca D, Cecchini M, Massantini R (2015) Feasibility of NIR spectroscopy to detect olive fruit infested by Bactrocera oleae. Postharvest Biol Technol 99(6):58–62. https://doi.org/10.1016/j.postharvbio.2014.07.015
Oliveira GAD, Castilhos FD, Bureau S (2014) Comparison of NIR and MIR spectroscopic methods for determination of individual sugars, organic acids and carotenoids in passion fruit. Food Res Int 60(6):154–162. https://doi.org/10.1016/j.foodres.2013.10.051
Pan L, Zhang Q, Zhang W, Sun Y, Hu P, Tu K (2016) Detection of cold injury in peaches by hyperspectral reflectance imaging and artificial neural network. Food Chem 192:134–141. https://doi.org/10.1016/j.foodchem.2015.06.106
Pearson TC, Wicklow DT (2006) Detection of corn kernels infected by fungi. Trans ASABE 49(4):1235–1245. https://doi.org/10.13031/2013.21723
Shen F, Wu Q, Liu P, Jiang X, Fang Y, Cao C (2018) Detection of aspergillus, spp. contamination levels in peanuts by near infrared spectroscopy and electronic nose. Food Control 93:1–8. https://doi.org/10.1016/j.foodcont.2018.05.039
Siedliska A, Baranowski P, Zubik M, Mazurek W, Sosnowska B (2018) Detection of fungal infections in strawberry fruit by VNIR/SWIR hyperspectral imaging. Postharvest Biol Technol 139:115–126. https://doi.org/10.1016/j.postharvbio.2018.01.018
Sun Y, Gu X, Sun K, Hu H, Xu M, Wang Z et al (2017a) Hyperspectral reflectance imaging combined with chemometrics and successive projections algorithm for chilling injury classification in peaches. LWT Food Sci Technol 75:557–564. https://doi.org/10.1016/j.lwt.2016.10.006
Sun Y, Wang Y, Xiao H, Gu X, Pan L, Tu K (2017b) Hyperspectral imaging detection of decayed honey peaches based on their chlorophyll content. Food Chem 235:194–202. https://doi.org/10.1016/j.foodchem.2017.05.064
Thornton CR, Slaughter DC, Davis RM (2010) Detection of the sour-rot pathogen Geotrichum candidum in tomato fruit and juice by using a highly specific monoclonal antibody-based ELISA. Int J Food Microbiol 143(3):166–172. https://doi.org/10.1016/j.ijfoodmicro.2010.08.012
Williams PJ, Geladi P, Britz TJ, Manley M (2012) Investigation of fungal development in maize kernels using NIR hyperspectral imaging and multivariate data analysis. J Cereal Sci 55(3):272–278. https://doi.org/10.1016/j.jcs.2011.12.003
Xu JL, Sun DW (2018) Computer vision detection of salmon muscle gaping using convolutional neural network features. Food Anal Methods 11(1):34–47. https://doi.org/10.1007/s12161-017-0957-4
Zhu N, Lin M, Nie Y, Wu D, Chen K (2016) Study on the quantitative measurement of firmness distribution maps at the pixel level inside peach pulp. Comput Electron Agric 130:48–56. https://doi.org/10.1016/j.compag.2016.09.018
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The authors would like to thank the National Natural Science Foundation of China (NSFC: 31671925; 31671926) for financial support and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) and the 2017’ Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX17_0631).
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Qiang Liu declares that he has no conflict of interest. Dandan Zhou declares that she has no conflict of interest. Siying Tu declares that she has no conflict of interest. Hui Xiao declares that she has no conflict of interest. Bin Zhang declares that he has no conflict of interest. Ye Sun declares that she has no conflict of interest. Leiqing Pan declares that he has no conflict of interest. Kang Tu declares that he has no conflict of interest.
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Liu, Q., Zhou, D., Tu, S. et al. Quantitative Visualization of Fungal Contamination in Peach Fruit Using Hyperspectral Imaging. Food Anal. Methods 13, 1262–1270 (2020). https://doi.org/10.1007/s12161-020-01747-x
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DOI: https://doi.org/10.1007/s12161-020-01747-x