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Malassezia and Staphylococcus dominate scalp microbiome for seborrheic dermatitis

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Abstract

Seborrheic dermatitis (SD) is a common disease of the human scalp that causes physical damage and psychological problems for patients. Studies have indicated that dysbiosis of the scalp microbiome results in SD. However, the specific fungal and bacterial microbiome changes related to SD remain elusive. To further investigate the fungal and bacterial microbiome changes associated with SD, we recruited 57 SD patients and 53 healthy individuals and explored their scalp microbiomes using next generation sequencing and the QIIME and LEfSe bioinformatics tools. Skin pH, sebum secretion, hydration, and trans-epidermal water loss (TWEL) were also measured at the scalp. We found no statistically significant differences between the normal and lesion sites in SD patients with different subtypes of dandruff and erythema. However, the fungal and bacterial microbiome could differentiate SD patients from healthy controls. The presence of Malassezia and Aspergillus was both found to be potential fungal biomarkers for SD, while Staphylococcus and Pseudomonas were found to be potential bacterial biomarkers. The fungal and bacterial microbiome were divided into three clusters through co-abundance analysis and their correlations with host factors indicated the interactions and potential cooperation and resistance between microbe communities and host. Our research showed the skin microbe dysbiosis of SD and highlighted specific microorganisms that may serve as potential biomarkers of SD. The etiology of SD is multi-pathogenetic-dependent on the linkage of several microbes with host. Scalp microbiome homeostasis could be a promising new target in the risk assessment, prevention, and treatment of SD disease.

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References

  1. Borda LJ, Wikramanayake TC (2015) Seborrheic dermatitis and dandruff: a comprehensive review. J Clin Investig Dermatol 3(2):1–22

    Google Scholar 

  2. Mameri ACA, Carneiro S, Mameri LMA, Telles da Cunha JM, Ramos-E-Silva M (2017) History of seborrheic dermatitis: conceptual and clinico-pathologic evolution. Skinmed 15(3):187–194

    PubMed  Google Scholar 

  3. Sanders MGH, Pardo LM, Franco OH, Ginger RS, Nijsten T (2018) Prevalence and determinants of seborrhoeic dermatitis in a middle-aged and elderly population: the Rotterdam Study. Br J Dermatol 178(1):148–153

    Article  CAS  PubMed  Google Scholar 

  4. DeAngelis YM, Gemmer CM, Kaczvinsky JR, Kenneally DC, Schwartz JR, Dawson TL Jr (2005) Three etiologic facets of dandruff and Seborrheic dermatitis: Malassezia fungi, sebaceous lipids, and individual sensitivity. J Investig Dermatol Symp Proc 10(3):295–297

    Article  PubMed  Google Scholar 

  5. Park T, Kim HJ, Myeong NR, Lee HG, Kwack I, Lee J, Kim BJ, Sul WJ, An S (2017) Collapse of human scalp microbiome network in dandruff and seborrheic dermatitis. Exp Dermatol 26(9):835–838

    Article  PubMed  Google Scholar 

  6. Xu Z, Wang Z, Yuan C, Liu X, Yang F, Wang T, Wang J, Manabe K, Qin O, Wang X, Zhang Y, Zhang M (2016) Dandruff is associated with the conjoined interactions between host and microorganisms. Sci Rep 6(1):24877

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Park HK, Ha MH, Park SG, Kim MN, Kim BJ, Kim W (2012) Characterization of the fungal microbiota (mycobiome) in healthy and dandruff-afflicted human scalps. PLoS ONE 7(2):e32847

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Saunders CW, Scheynius A, Heitman J (2012) Malassezia fungi are specialized to live on skin and associated with dandruff, eczema, and other skin diseases. PLoS Pathog 8(6):e1002701

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Clavaud, Jourdain R, Bar-Hen A, Tichit M, Bouchier C, Pouradier F, EI Rawadi C, Guillot J, Ménard-Szczebara F, Breton L, Latgé JP, Mouyna I (2013) Dandruff is associated with disequilibrium in the proportion of the major bacterial and fungal populations colonizing the scalp. PLoS ONE 8(3):e58203

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Turner GA, Hoptroff M, Harding CR (2012) Stratum corneum dysfunction in dandruff. Int J Cosmet Sci 34(4):298–306

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Soares RC, Camargo-Penna PH, de Moraes VC, De Vecchi R, Clavaud C, Breton L, Braz A, Paulino LC (2016) Dysbiotic bacterial and fungal communities not restricted to clinically affected skin sites in dandruff. Front Cell Infect Microbiol 6:157

    Article  PubMed  PubMed Central  Google Scholar 

  12. Rippke F, Schreiner V, Doering T, Maibach HI (2004) Stratum corneum pH in atopic dermatitis: impact on skin barrier function and colonization with Staphylococcus aureus. Am J Clin Dermatol 5(4):217–223

    Article  PubMed  Google Scholar 

  13. Harding CR, Moore AE, Rogers JS, Meldrum H, Scott AE, McGlone FP (2002) Dandruff: a condition characterized by decreased levels of intercellular lipids in scalp stratum corneum and impaired barrier function. Arch Dermatol Res 294(5):221–230

    Article  CAS  PubMed  Google Scholar 

  14. Bacon RA, Mizoguchi H, Schwartz JR (2014) Assessing therapeutic effectiveness of scalp treatments for dandruff and Seborrheic dermatitis, part 1: a reliable and relevant method based on the adherent scalp flaking score (ASFS). J Dermatol Treat 25(3):232–236

    Article  Google Scholar 

  15. Kerr K, Darcy T, Henry J, Mizoguchi H, Schwartz JR, Morrall S, Filloon T, Wimalasena R, Fadayel G, Mills KJ (2011) Epidermal changes associated with symptomatic resolution of dandruff: biomarkers of scalp health. Int J Dermatol 50(1):102–113

    Article  CAS  PubMed  Google Scholar 

  16. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Gelfand DH, Sninsky JJ, White TJ, Innis MA (eds) PCR protocols. A guide to methods and applications. Academic, San Diego, pp 315–322

    Google Scholar 

  17. Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett WS, Huttenhower C (2011) Metagenomic biomarker discovery and explanation. Genome Bio 12(6):R60

    Article  Google Scholar 

  18. Grice EA (2014) The skin microbiome: potential for novel diagnostic and therapeutic approaches to cutaneous disease. Semin Cutan Med Surg 33(2):98–103

    Article  PubMed  PubMed Central  Google Scholar 

  19. Schwartz JR, Messenger AG, Tosti A, Todd G, Hordinsky M, Hay RJ, Wang X, Zachariae C, Kerr KM, Henry JP, Rust RC, Robinson MK (2013) A comprehensive pathophysiology of dandruff and seborrheic dermatitis—towards a more precise definition of scalp health. Acta Derm Venereol 93(2):131–137

    Article  CAS  PubMed  Google Scholar 

  20. Kim SY, Kim SH, Kim SN, Kim AR, Kim YR, Kim MJ, Park WS, Lee JH, Jung WH, Lee YW, Choe YB, Ahn KJ (2016) Isolation and identification of Malassezia species from Chinese and Korean patients with seborrheic dermatitis and in vitro studies on their bioactivity on sebaceous lipids and IL-8 production. Mycoses 59(5):274–280

    Article  CAS  PubMed  Google Scholar 

  21. Sommer B, Overy DP, Kerr RG (2015) Identification and characterization of lipases from Malassezia restricta, a causative agent of dandruff. FEMS Yeast Res 15(7):078

    Article  Google Scholar 

  22. Brinkac L, Clarke TH, Singh H, Greco C, Gomez A, Torralba MG, Frank B, Nelson KE (2018) Spatial and environmental variation of the human hair microbiota. Sci Rep 8(1):9017

    Article  PubMed  PubMed Central  Google Scholar 

  23. Hay RJ (2011) Malassezia, dandruff and seborrhoeic dermatitis: an overview. Br J Dermatol 165(Suppl 2):2–8

    Article  PubMed  Google Scholar 

  24. Sampaio AL, Mameri AC, Vargas TJ, Ramos-e-Silva M, Nunes AP, Carneiro SC (2011) Seborrheic dermatitis. An Bras Dermatol 86:1061–1071

    Article  PubMed  Google Scholar 

  25. Dawson TL Jr (2007) Malassezia globosa and restricta: breakthrough understanding of the etiology and treatment of dandruff and seborrheic dermatitis through whole-genome analysis. J Investig Dermatol Symp Proc 12:15–19

    Article  CAS  PubMed  Google Scholar 

  26. Saxena R, Mittal P, Clavaud C, Dhakan DB, Hegde P, Veeranagaiah MM, Saha S, Souverain L, Roy N, Breton L, Misra N, Sharma VK (2018) Comparison of healthy and dandruff scalp microbiome reveals the role of commensals in scalp health. Front Cell Infect Microbiol 8:346

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Gaitanis G, Magiatis P, Stathopoulou K, Bassukas ID, Alexopoulos EC, Velegraki A, Skaltsounis A-L (2008) AhR ligands, malassezin, and indolo[3,2-b]carbazole are selectively produced by Malassezia furfur strains isolated from seborrheic dermatitis. J Invest Dermatol 128(7):1620–1625

    Article  CAS  PubMed  Google Scholar 

  28. Tamer F, Yuksel M, Sarifakioglu E, Karabag Y (2018) Staphylococcus aureus is the most common bacterial agent of the skin flora of patients with seborrheic dermatitis. Dermatol Pract Concept 8(2):80–84

    Article  PubMed  PubMed Central  Google Scholar 

  29. Tanaka A, Cho O, Saito C, Saito M, Tsuboi R, Sugita T (2016) Comprehensive pyrosequencing analysis of the bacterial microbiota of the skin of patients with seborrheic dermatitis. Microbiol Immunol 60:521–526

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This work is supported by the National Science and Technology Major Project of China (Grant No. 2016ZX10004001-009).

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Authors and Affiliations

  1. Beijing Institute of Biotechnology, Beijing, 10071, China

    Qingbin Lin, Lihua Hou & Wei Chen

  2. Ben May Department for Cancer Research, The University of Chicago, Chicago, IL, 60637, USA

    Ananth Panchamukhi

  3. Division of Laboratory Medicine, Microbiome Medicine Center, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, Guangdong, China

    Pan Li, Wang Shan & Hongwei Zhou

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  1. Qingbin Lin
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  2. Ananth Panchamukhi
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  3. Pan Li
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  7. Wei Chen
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Correspondence to Wei Chen.

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449_2020_2333_MOESM1_ESM.tif

Supplementary file1 The most abundant phyla for fungal (a) and bacteria (c), the predominant 8 genera for fungal(b) and bacteria (d), which shared by the Healthy group and the SD patient group, and the subtype groups of the SD patients (TIF 1919 kb)

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Lin, Q., Panchamukhi, A., Li, P. et al. Malassezia and Staphylococcus dominate scalp microbiome for seborrheic dermatitis. Bioprocess Biosyst Eng 44, 965–975 (2021). https://doi.org/10.1007/s00449-020-02333-5

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  • DOI: https://doi.org/10.1007/s00449-020-02333-5

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