Issue 17, 2020
From the journal:

Analytical Methods

Droplet image analysis with user-friendly freeware CellProfiler†

Simona Bartkova, ORCID logo a   Marko Vendelin, ORCID logo b   Immanuel Sanka, ORCID logo a   Pille Pata ORCID logo a  and  Ott Scheler ORCID logo *a  

* Corresponding authors

a Department of Chemistry and Biotechnology, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia
E-mail: ott.scheler@taltech.ee

b Department of Cybernetics, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia

Abstract

Droplet microfluidic assays are rapidly gaining popularity because they enable analysis of biochemical reactions, individual cells or small cell populations with high sensitivity, precision and accuracy in a high-throughput manner. Nonetheless, there is a demand for user-friendly and low-cost droplet analysis technology. In this article, we meet this demand by developing two droplet analysis pipelines via free open-source software CellProfiler (CP) and its companion (CellProfiler Analyst, CPA). To illustrate the competence of the pipelines as an independent, and freely accessible droplet analysis tool for any researcher without the need of programming skills, we show (i) droplet digital quantification of viable fluorescent bacteria using single-color images and (ii) analysis of a multi-color fluorescence image with droplets containing different chemical compositions.

Graphical abstract: Droplet image analysis with user-friendly freeware CellProfiler

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Article information

DOI
https://doi.org/10.1039/D0AY00031K
Article type
Technical Note
Submitted
06 Jan 2020
Accepted
25 Mar 2020
First published
25 Mar 2020

Anal. Methods, 2020,12, 2287-2294

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Droplet image analysis with user-friendly freeware CellProfiler

S. Bartkova, M. Vendelin, I. Sanka, P. Pata and O. Scheler, Anal. Methods, 2020, 12, 2287 DOI: 10.1039/D0AY00031K

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