Research ArticleConjugated linoleic acid attenuates 2,4-dinitrofluorobenzene-induced atopic dermatitis in mice through dual inhibition of COX-2/5-LOX and TLR4/NF-κB signaling
Section snippets
Chemicals and reagents
DNFB (purity 99%) was purchased from Alfa Aesar (Heysham, UK). Olive oil was obtained from Aladdin (Shanghai, China). DEX (purity 98%) was supplied by Saen Chemical Technology Co. Ltd. (Shanghai, China). CLA (containing 50:50 mixture of cis-9, trans-11 and trans-10, cis-12 CLA isomers) was obtained from Qingdao Auhai Biotech Co., Ltd. (Shangdong, China). Enzyme-linked immunosorbent assay (ELISA) kits were produced by Bioswamp Life Science Lab. (Wuhan, China). CD4, cyclooxygenase-2 (COX-2),
CLA alleviates DNFB-induced AD-like symptoms
Fig. 1C indicates that repeated application of DNFB to mouse dorsal skin induced clinical characteristics of AD, such as dryness, erythema, erosion, edema/excoriation and scaling on the final day of the experiment. Co-treatment of CLA ameliorated these phenotypes in skin lesions, with most of the mice exhibiting slightly scarred or dry skin. Meanwhile, the development of skin lesions was inhibited by oral application of CLA (Fig. 2A). On day 29, CLA markedly decreased DNFB-induced increases in
Discussion
AD is one of the most common relapsing inflammatory skin diseases prevailing around the world, which is characterized by Th1/Th2 imbalance, IgE hypersensitivity and typical clinical signs of chronic pruritus and eczematous skin lesions [2,39]. Whigham et al. [17] proved that CLA was able to reduce antigen-induced histamine and PGE2 release from tracheas of guinea pigs sensitized to chicken egg ovalbumin, suggesting the potential therapeutic effect of CLA on AD. To place the results of the
Conflicts of interest
The authors declare no conflict of interest.
References (57)
- et al.
Atopic dermatitis
Lancet
(2016) - et al.
Management of difficult-to-treat atopic dermatitis
J Allergy Clin Immunol Pract
(2013) - et al.
(R)-(+)-pulegone suppresses allergic and inflammation responses on 2,4-dinitrochlorobenzene-induced atopic dermatitis in mice model
J Dermatol Sci
(2018) - et al.
Atopic dermatitis: immune deviation, barrier dysfunction, IgE autoreactivity and new therapies
Allergol Int
(2017) - et al.
Conjugated linoleic acid prevents the development of essential hypertension in spontaneously hypertensive rats
Biochem Biophys Res Commun
(2004) Cows' milk fat components as potential anticarcinogenic agents
J Nutr
(1997)- et al.
Biological effects of conjugated linoleic acids in health and disease
J Nutr Biochem
(2006) - et al.
Modulation of inflammation and immunity by dietary conjugated linoleic acid
Eur J Pharmacol
(2016) - et al.
Anti-allergic effects of novel sulfated polysaccharide sacran on mouse model of 2,4-Dinitro-1-fluorobenzene-induced atopic dermatitis
Int J Biol Macromol
(2018) - et al.
Anti-pruritic and anti-inflammatory effects of oxymatrine in a mouse model of allergic contact dermatitis
J Dermatol Sci
(2018)