Mechanical Characterization of Cells and Microspheres Sorted by Acoustophoresis with In-Line Resistive Pulse Sensing

Antoine Riaud, Wei Wang, Anh L.P. Thai, and Valerie Taly
Phys. Rev. Applied 13, 034058 – Published 24 March 2020
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Abstract

Resistive pulse sensing (RPS) is a key label-free technology to measure particles and single-cell size distribution. As a growing corpus of evidence supports that cancer cells exhibit distinct mechanical phenotypes from healthy cells, expanding the method from size to mechanical sensing could represent a pertinent and innovative tool for cancer research. In this paper, we infer the cells’ compressibility by using acoustic radiation pressure to deflect flowing cells in a microchannel, and use RPS to sense the subpopulations of cells and particles at each acoustic power level. Compared to current acoustic cell phenotyping apparatus based on video cameras, the proposed approach is not limited by optical diffraction, frame rate, data storage, or processing speed, and may ultimately constitute a step forward towards point-of-care acousto-electrical phenotyping and acoustic phenotyping of liquid biosamples.

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  • Received 10 November 2019
  • Revised 21 January 2020
  • Accepted 7 February 2020

DOI:https://doi.org/10.1103/PhysRevApplied.13.034058

© 2020 American Physical Society

Physics Subject Headings (PhySH)

Fluid DynamicsInterdisciplinary PhysicsPhysics of Living Systems

Authors & Affiliations

Antoine Riaud1,*,‡, Wei Wang2, Anh L.P. Thai1,§, and Valerie Taly1,†

  • 1Centre de Recherche des Cordeliers, INSERM, CNRS, Sorbonne Université, USPC, Université Paris Descartes, Université Paris Diderot, F-75006-Equipe labellisée Ligue Nationale contre le cancer, Paris, France
  • 2ASIC and System State Key Laboratory, School of Microelectronics, Fudan University, Shanghai 200433, China

  • *antoine.riaud@centraliens-lille.org
  • valerie.taly@parisdescartes.fr
  • Current affiliation: ASIC and System State Key Laboratory, School of Microelectronics, Fudan University, Shanghai 200433, China. http://homepage.fudan.edu.cn/ariaud/
  • §Current affiliation: The Physics of Living Matter Group, Department of Physics and Materials Sciences, University of Luxembourg.

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Vol. 13, Iss. 3 — March 2020

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