Cloning, expression and comparison of the properties of Scy p 9, a Scylla paramamosain allergen

Xin-Rong He; Yi-Meng Cheng; Yang Yang; Jie-Jing Xie; Ka-Hou Chu; Yong-Xia Zhang; Gui-Xia Chen; Hong Liu; Min-Jie Cao; Guang-Ming Liu

doi:10.1039/D0FO00004C

Cloning, expression and comparison of the properties of Scy p 9, a Scylla paramamosain allergen†

Xin-Rong He,^a Yi-Meng Cheng,^a Yang Yang,^ab Jie-Jing Xie,^a Ka-Hou Chu,^c Yong-Xia Zhang,^a Gui-Xia Chen,^d Hong Liu,^a Min-Jie Cao^a and Guang-Ming Liu

*^a

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* Corresponding authors

^a College of Food and Biological Engineering, Xiamen Key Laboratory of Marine Functional Food, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Jimei University, Xiamen, Fujian, China
E-mail: gmliu@jmu.edu.cn
Fax: +86-592-6180470
Tel: +86-592-6180378

^b College of Environment and Public Health, Xiamen Huaxia University, 288 Tianma Road, Xiamen, Fujian, China

^c School of Life Sciences, The Chinese University of Hong Kong, Sha Tin, Hong Kong, China

^d Women and Children's Hospital Affiliated to Xiamen University, Xiamen, Fujian 361003, China

Abstract

This study investigated the properties of Scy p 9 in mud crab (Scylla paramamosain). The gene sequence of filamin C obtained from crabs, which was denoted as Scy p 9, contains a 2544 bp open reading frame and encodes 848 amino acid residues. Recombinant Scy p 9 (rScy p 9) is expressed in Escherichia coli, which exhibits tertiary structure changes, and the IgE binding activity of rScy p 9 is higher than that of native Scy p 9 (nScy p 9). Moreover, this study explored the possibility of the presence and cross-reactivity of filamin C in 8 shellfish. IgE-specific binding to nScy p 9 and rScy p 9 in patients allergic to shellfish revealed that rScy p 9 was more sensitive than nScy p 9. The gene sequence of filamin C fills in the blank in shellfish. This study contributes to the understanding of the properties of Scy p 9, and the results indicate that rScy p 9 can be used as a candidate for component-resolved diagnosis in shellfish.

This article is part of the themed collection: Food & Function Recent HOT articles

Food & Function

Cloning, expression and comparison of the properties of Scy p 9, a Scylla paramamosain allergen†

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Cloning, expression and comparison of the properties of Scy p 9, a Scylla paramamosain allergen

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