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Gram scale synthesis of QD450 core–shell quantum dots for cellular imaging and sorting

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Abstract

In this study, we described an easy and one-pot synthesis technique for high-quality CdZnS/ZnS core/shell quantum dots, which exhibit greater fluorescence at λ450 nm with steady quantum yields in both organic and aqueous solvents. At lower concentrations, they showed a decrease in cytotoxicity, which is more suitable for the biocompatible applications. Further, we successfully demonstrated the fluorescence imaging and sorting through the surface display of the quantum dots in MCF-7 and HELA cell lines using confocal microscopy and flow cytometer studies.

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Acknowledgments

Satyanarayana Swamy Vyshnava would like to thank for the fellowship provided to research and stay in Taiwan by Taiwan International Graduate Program (TIGP-NANO-2015) Academia Sinica, Taiwan. Facilities provided include chemical synthesis, cell culture and confocal Imaging by Prof Peilin Chen, RCAS, Academia Sinica; FEG-TEM by Institute of Physics, Academia Sinica; LSRII Flow Cytometry, Institute of Biomedical Sciences, Academia Sinica; FL290 PL Decay, Institute of Chemistry, Academia Sinica; UV- Visible Absorption Spectroscopy facility provided by Prof Chia-Fu Chou, Institute of Physics, Academia Sinica.

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Authors and Affiliations

  1. Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei, Taiwan

    Satyanarayana Swamy Vyshnava

  2. Nano Science and Technology Program, Taiwan International Graduate Program, Academia Sinica and National Taiwan University, Taipei, Taiwan

    Satyanarayana Swamy Vyshnava

  3. Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan

    Satyanarayana Swamy Vyshnava

  4. Institute of Physics, Academia Sinica, Taipei, Taiwan

    Satyanarayana Swamy Vyshnava

  5. Department of Microbiology, Sri Krishnadevaraya University, Anantapuram, A.P, India

    Gayathri Pandluru, Dileep Kumar Kanderi & Rajashekar Reddy Bontha

  6. Department of Genetics and Biotechnology, Osmania University, Hyderabad, T.S, India

    Shiva Prasad Panjala, Swathi Banapuram & Roja Rani Anupalli

  7. Institute of Comparative Medicine, Yangzhou University, Yangzhou, China

    Kameshpandian Paramasivam

  8. Department of Biotechnology, Sri Krishnadevaraya University, Anantapuram, A.P, India

    Muralidhara Rao Dowlatabad

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  1. Satyanarayana Swamy Vyshnava
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  2. Gayathri Pandluru
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Correspondence to Satyanarayana Swamy Vyshnava.

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Vyshnava, S.S., Pandluru, G., Kanderi, D.K. et al. Gram scale synthesis of QD450 core–shell quantum dots for cellular imaging and sorting. Appl Nanosci 10, 1257–1268 (2020). https://doi.org/10.1007/s13204-020-01261-w

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