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A Spectroscopic Study of Changes in the Secondary Structure of Proteins of Biological Fluids of the Oral Cavity by Synchrotron Infrared Microscopy

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Abstract

On the basis of the data of infrared spectroscopy with synchrotron radiation, the secondary structure of proteins of the dentinal and gingival fluids during the development of cariosity in deep dentin tissues is studied. It is shown that the change in the shape of the profile of the amide I band in the region of 1700‒1605 cm–1 is associated both with a change in the ratio of the integrated absorption intensities of the α‑helix and β-sheet secondary structures and with the position of the β-coil and β-sheet components in the spectrum. It is established that the α-helix/β-sheet ratio for both dentinal and gingival fluids is below the threshold level, at which significant changes in the secondary structure of proteins of biological fluids are observed, unequivocally indicating the development of pathology in hard dental tissues. The features that we discovered in the profile of the amide I band of biological fluids of the oral cavity, together with the spectral markers of the development of cariosity in dentin, are reliable spectroscopic signatures of the pathology and can be detected using the gingival fluid.

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ACKNOWLEDGMENTS

This study was partially performed using the infrared microspectroscopy (IRM) beamline at the Australian Synchrotron.

Funding

This study was supported by a grant from the Russian Science Foundation, project no. 16-15-00003.

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Authors and Affiliations

  1. Voronezh State University, 394036, Voronezh, Russia

    P. V. Seredin & D. L. Goloshchapov

  2. Burdenko Voronezh State Medical University, 394036, Voronezh, Russia

    Yu. A. Ippolitov

  3. The Australian Synchrotron (Synchrotron Light Source Australia Pty LTD), VIC 3168, Clayton, Australia

    Jitraporn (Pimm) Vongsvivut

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  1. P. V. Seredin
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  3. Yu. A. Ippolitov
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Correspondence to P. V. Seredin.

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COMPLIANCE WITH ETHICAL STANDARDS

All procedures performed in this study with human participation comply with the ethical standards of the 1964 Helsinki Declaration and its subsequent amendments or with comparable ethical standards. Informed voluntary consent was received from each participant included in the study.

CONFLICT OF INTEREST

The authors declare that they have no conflict of interest.

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Translated by O. Kadkin

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Seredin, P.V., Goloshchapov, D.L., Ippolitov, Y.A. et al. A Spectroscopic Study of Changes in the Secondary Structure of Proteins of Biological Fluids of the Oral Cavity by Synchrotron Infrared Microscopy. Opt. Spectrosc. 127, 1002–1010 (2019). https://doi.org/10.1134/S0030400X19120221

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