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Magnificamide Is a New Effective Mammalian α-Amylase Inhibitor

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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Abstract

Recombinant analogue of the sea anemone Heteractismagnifica peptide was obtained, and the kinetic parameters of its interaction with mammalian α-amylases were determined. Magnificamide inhibits α-amylases significantly stronger than the medical drug acarbose (PrecoseTM or GlucobayTM). Magnificamide is assumed to find application as a drug for prevention and treatment of metabolic disorders and type 2 diabetes mellitus.

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ACKNOWLEDGMENTS

Molecular weights of the peptides were determined using the equipment of the Far Eastern Center of Molecular Structural Studies (NMR and mass spectrometry) of the Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences.

Funding

This work was supported by the Russian Foundation for Basic Research (project no. 18-38-00389 (preparation of recombinant peptide and determination of Ki)).

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Authors and Affiliations

  1. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 690022, Vladivostok, Russia

    O. V. Sintsova, E. V. Leychenko, I. N. Gladkikh, A. P. Kalinovskii, M. M. Monastyrnaya & E. P. Kozlovskaya

  2. School of Natural Sciences, Far Eastern Federal University, 690091, Vladivostok, Russia

    A. P. Kalinovskii

Authors
  1. O. V. Sintsova
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  2. E. V. Leychenko
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  3. I. N. Gladkikh
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  4. A. P. Kalinovskii
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  5. M. M. Monastyrnaya
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  6. E. P. Kozlovskaya
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Corresponding author

Correspondence to O. V. Sintsova.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by M. Batrukova

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Sintsova, O.V., Leychenko, E.V., Gladkikh, I.N. et al. Magnificamide Is a New Effective Mammalian α-Amylase Inhibitor. Dokl Biochem Biophys 489, 385–387 (2019). https://doi.org/10.1134/S1607672919060097

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  • DOI: https://doi.org/10.1134/S1607672919060097

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