Abstract
Sixteen variants of test systems (two-site sandwich and competitive enzyme-linked immunosorbent assay (ELISA)) were studied to determine the concentration of microfungi, some of which are known opportunistic pathogens for humans, in soil extracts. The correlation of the amount of fungal biomass (the length of the growing mycelium) with the concentrations of antigens detected by the two-site sandwich ELISA method and the competitive ELISA method was evaluated. It has been shown that the methods are highly effective in the case of long incubation (more than 5 days) of soil samples and are ineffective for short-term incubation, because there was no correlation between hypha length and antigen concentration during the first day of cultivation. The perspectivity of ELISA methods for the detection of some soil microfungi from genera Aspergillus (Asp. flavus, Asp. fumigatus, Asp. niger), Fusarium (F. solani, F. poae), and others (Alternaria alternata, Phoma lingham, Mucor hiemalis) was shown. The competitive ELISA method was more efficient than two-site sandwich ELISA.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Wu, L., Li, G., Xu, X., Zhu, L., Huang, R., and Chen, X., TrAC, Trends Anal. Chem. (Pers. Ed.), 2019, vol. 113, pp. 140–156.
Omori, A.M., Ono, E.Y.S., Hirozawa, M.T., de Souza Suguiura, I.M., Hirooka, E.Y., Pelegrinelli Fungaro, M.H., and Ono, M.A., Toxins, 2019, vol. 11, article 48.
Schlaeppi, J.M., Foery, W., and Ramsteiner, K., J. Agric. Food Chem., 1989, vol. 37, no. 6, pp. 1532–1538.
Hollaway, K.L., Kookana, R.S., McQuinn, D.J., Moerkerk, M.R., Noy, D.M., and Smal, M.A., Suelo Planta, 1999, vol. 39, no. 5, pp. 383–397.
Xu, Z.-L., Wang, H., Shen, Y.-D., Nichkova, M., Lei, H.-T., Beier, R.C., Zheng, W.-X., Yang, J.-Y., She, Z.-G., and Sun, Y.-M., Analyst, 2011, vol. 136, no. 12, pp. 2512–2520.
Sapozhnikova, Y., Simons, T., and Lehotay, S.J., J. Agric. Food Chem., 2015, vol. 63, no. 18, pp. 4429–4434.
Xu, Z.-L., Deng, H., Deng, X.-F., Yang, J.-Y., Jiang, Y.-M., Zeng, D.-P., Huang, F., Shen, Y.-D., Lei, H.-T., Wang, H., and Sun, Y.-M., Food Chem., 2012, vol. 131, no. 4, pp. 1569–1576.
Qian, G., Wang, L., Wu, Y., Zhang, Q., Sun, Q., Liu, Y., and Liu, F., Food Chem., 2009, vol. 117, no. 2, pp. 364–370.
Rengasamy, P.T., Kantharimuthu, A., Balaraman, M., and Sivanesan, S., African J. Food Sci., 2016, vol. 10, no. 3, pp. 41–47.
Li, C., Zhang, Y., Eremin, S.A., Yakup, O., Yao, G., and Zhang, X., Food Chem., 2017, vol. 227, no. 1, pp. 48–54.
Johnson, J.C. and Van Emon, J.M., Anal. Chem., 1996, vol. 68, no. 1, pp. 162–169.
Aga, D.S., O’Connor, S., Ensley, S., Payero, J.O., Snow, D., and Tarkalson, D., J. Agric. Food Chem., 2005, vol. 53, no. 18, pp. 7165–7171.
Lisichkina, G.A., Kozhevin, P.A., and Zvyagintsev, D.G., Mikrobiologiya, 1983, vol. 52, no. 4, pp. 646–650.
Kirillova, N.P., Kozhevin, P.A., and Zvyagintsev, D.G., Mikrobiologiya, 1984, vol. 53, no. 1, pp. 117–122.
Fuhrmann, J. and Wollum, A.G., Appl. Environ. Microbiol., 1985, vol. 49, no. 4, pp. 1010–1013.
Lukin, S.A., Kozhevin, P.A., and Zvyagintsev, D.G., S-kh. Biol., 1987, no. 1, pp. 51–58.
Krol, M.J., Bashan, Y., and Gonzalez, L.E., Appl. Microbiol. Biotechnol., 1999, vol. 51, no. 2, pp. 262–266.
Efremenkova, L., Kozhevin, P., and Zvyagintsev, D., Microbiology, 1978, vol. 47, no. 6, pp. 916–918.
Musgrave, D.R., New Zealand J. Agric. Res., 1984, vol. 27, no. 2, pp. 283–288.
Wright, S.F., Morton, J.B., and Sworobuk, J.E., Appl. Environ. Microbiol., 1987, vol. 53, no. 9, pp. 2222–2225.
Aldwell, F.E.B., Hall, I.R., and Smith, J.M.B., Soil Biol. Biochem., 1983, vol. 15, no. 3, pp. 377–378.
Gannibal, F.B., Toxigenicity and pathogenicity of fungi of the genus Alternaria in cereals, in Laboratoriya mikologii i fitopatologii im. A.A. Yachevskogo Vserossiiskogo instituta zachshity rastenii. Istoriya i sovremennost’ (Yachevskii Laboratory of Mycology and Phytopathology, All-Russia Institute of Plant Protection: History and Modernity), Dmitriev, A.P., Ed., St. Petersburg: VIZR, 2007, pp. 82–93.
Marfenina, O.E., Bubnova, E.N., Semenova, T.A., Ivanova, A.E., and Danilogorskaya, A.A., Mikol. Fitopatol., 2014, vol. 48, no. 3, pp. 10–24.
Kul'ko, A.B., Tuberkulez Bol. Llegkikh, 2017, vol. 95, no. 3, pp. 54–60.
Richardson, M.D. and Page, I.D., Med. Mycol., 2016, vol. 55, no. 1, pp. 48–55.
Hanssen, J.F., Thingstad, T.F., and Goksoyr, J., Oikos, 1974, vol. 25, no. 1, pp. 102–107.
Funding
This work was supported by the Ministry of Education and Science of Russian Federation within the framework of the project “The development of the testing systems for the express immunodetection of phytopathogenic fungi in cereals in order to create the prerequisites for their successful commercialization in Russia and the world,” project no. RFMEFI60717X0185.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
Additional information
Translated by M. Bibov
Rights and permissions
About this article
Cite this article
Ivanova, A.E., Shutova, A.S., Gannesen, A.V. et al. Determination of the Mycelium and Antigens of a Number of Micromycetes in Soil Extracts via Enzyme-Linked Immunosorbent Assay. Appl Biochem Microbiol 56, 72–77 (2020). https://doi.org/10.1134/S0003683820010081
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0003683820010081