Skip to main content
SpringerLink
Log in

Effects of Substrate and Cultivation Duration on the Productivity, Biological Activity, and Chromatography Profiles of Extracts Obtained from Stagonospora cirsii S-47

  • Published:
Applied Biochemistry and Microbiology Aims and scope Submit manuscript

Abstract

In order to determine the possible toxic properties of a potential mycoherbicide based on the fungus Stagonospora cirsii S-47, the effects of solid and liquid media composition on the yield of extractive matter (YEM), the spectrum of biological activity, and the chromatography profiles of extracts obtained from cultures of this fungus were studied. The maximal YEM (~250 mg/L) was obtained from filtrates taken from a 2-week culture of S. cirsii S-47 grown on a sucrose–soy meal medium. The same time was optimal for the production of maximal YEM (~1.5 g/kg) when the fungus was grown on pearl-barley substrate. The fungal extracts showed phytotoxic, antimicrobial, cytotoxic, and antiesterase activity. Extracts from culture filtrates generally demonstrated a higher level and wider spectrum of biological activity than extracts from solid S. cirsii S-47 culture. The maximal phytotoxic and antimicrobial activity was found in dichloromethane extracts from the filtrate of 3-week fungal culture on Czapek medium, while extracts from two-week-old culture strongly inhibited carboxyl esterase. The maximal cytotoxic activity against the U251 cell line was found in ethyl acetate extract from a 3-week culture of S. cirsii S-47 produced on YMG liquid medium. The extract compositions varied considerably depending on the culture substrate.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.

Similar content being viewed by others

REFERENCES

  1. Berestetskiy, A.O., Vestn. Zashch. Rast., 2017, vol. 91, no. 1, pp. 5–12.

    Google Scholar 

  2. Berestetskiy, A.O., Appl. Biochem. Microbiol., 2008, vol. 44, no. 5, pp. 453–465.

    Article  CAS  Google Scholar 

  3. Ahmed, N.E., Sugimoto, Y., Babiker, A.G.T., Mohamed, O.E., Ma, Y., Inanaga, S., and Nakajima, H., Weed Res., 2001, vol. 49, no. 3, pp. 354–358.

    Article  CAS  Google Scholar 

  4. Abouzeid, M.A., Boari, A., Zonno, M.C., Vurro, M., and Evidente, A., Weed Res., 2004, vol. 52, no. 3, pp. 326–332.

    Article  CAS  Google Scholar 

  5. Anderson, K.I. and Hallett, S.G., Weed Res., 2004, vol. 52, no. 4, pp. 623–627.

    Article  CAS  Google Scholar 

  6. Hoagland, R.E., Weaver, M.A., and Boyette, C.D., Allelopathy J., 2007, vol. 19, no. 1, pp. 179–192.

    Google Scholar 

  7. Lee, H.B., Kim, J.-C., Hong, K.-S., and Kim, C.-J., Plant Pathol. J., 2008, vol. 24, no. 4, pp. 453–460.

    Article  CAS  Google Scholar 

  8. Nzioki, H.S., Oyosi, F., Morris, C.E., Kaya, E., Pilgeram, A.L., Baker, C.S., and Sands, D., Front. Plant Sci., 2016, vol. 7, p. 1121.

    Article  Google Scholar 

  9. Fumagalli, P., Andolfi, A., Avolio, F., Boari, A., Cimmino, A., and Finizio, A., Pest Manage. Sci., 2013, vol. 69, no. 7, pp. 850–856.

    Article  CAS  Google Scholar 

  10. Favilla, M., Macchia, L., Gallo, A., and Altomare, C., Food Chem. Toxicol., 2006, vol. 44, no. 11, pp. 1922–1931.

    Article  CAS  Google Scholar 

  11. Skrobek, A., Boss, D., Defago, G., Butt, T.M., and Maurhofer, M., Toxicol. Lett., 2006, vol. 161, no. 1, pp. 43–52.

    Article  CAS  Google Scholar 

  12. Boss, D., Maurhofer, M., Schlapfer, E., and Defago, G., FEMS Microbiol. Ecol., 2007, vol. 59, no. 1, pp. 194–205.

    Article  CAS  Google Scholar 

  13. Kouvelis, V.N., Wang, C., Skrobek, A., Pappas, K.M., Typas, M.A., and Butt, T.M., Mutat. Res., 2011, vol. 722, no. 1, pp. 1–6.

    Article  CAS  Google Scholar 

  14. Berestetskiy, A.O., Ivanova, A.N., Petrova, M.O., Prokof’eva, D.S., Stepanycheva, E.A., Uspanov, A.M., and Lednev, G.R., Arch. Microbiol., 2018, vol. 87, no. 2, pp. 200–214.

    CAS  Google Scholar 

  15. Deising, H.B., Gase, I., and Kubo, Y., J. Plant Dis. Prot., 2007, vol. 124. https://doi.org/10.1007/s41348-017-0109-5

  16. Evidente, A., Berestetskiy, A., Cimmino, A., Tuzi, A., Superchi, S., Melck, D., and Andolfi, A., J. Agric. Food Chem., 2009, vol. 57, no. 23, pp. 11168–11173.

    Article  CAS  Google Scholar 

  17. Andolfi, A., Cimmino, A., Vurro, M., Berestetskiy, A., Troise, C., Zonno, M.C., Motta, A., and Evidente, A., Phytochemistry, 2012, vol. 79, pp. 102–108.

    Article  CAS  Google Scholar 

  18. Sorensen, J.L. and Sondergaard, T.E., Int. J. Food Mirobiol., 2014, vol. 170, pp. 55–60.

    Article  Google Scholar 

  19. Shi, W., Tan, Y., Wang, S., Gardiner, D.M., De Saeger, S., Liao, Y., Wang, C.FanY., Wang, Z., and Wu, A., Toxins, 2017, vol. 9. https://doi.org/10.3390/toxins9010006

  20. RF Patent no. 2515899, 2014.

  21. RF Patent no. 2543665, 2015.

  22. Artokhin, K.S., Sornye rasteniya (Weed Plants), Moscow: Pechatnyi Gorod, 2007.

  23. Yuzikhin, O., Mitina, G., and Berestetskiy, A., J. Agric. Food Chem., 2007, vol. 55, no. 19, pp. 7707–7711.

    Article  CAS  Google Scholar 

  24. Rivero-Cruz, J.F., García-Aguirre, G., Cerda-García-Rojas, C., and Mata, R., Tetrahedron, 2000, vol. 56, no. 30, pp. 5337–5344.

    Article  Google Scholar 

  25. Evidente, A., Cimmino, A., Berestetskiy, A., Mitina, G., Andolfi, A., and Motta, A., J. Nat. Prod., 2008, vol. 71, pp. 31–34.

    Article  CAS  Google Scholar 

  26. Evidente, A., Cimmino, A., Berestetskiy, A., Andolfi, A., and Motta, A., J. Nat. Prod., 2008, vol. 71, no. 11, pp. 1897–1901.

    Article  CAS  Google Scholar 

  27. Greve, H., Schupp, P.J., Eguereva, E., Kehraus, S., and Konig, G.M., J. Nat. Prod., 2008, vol. 71, no. 9, pp. 1651–1653.

    Article  CAS  Google Scholar 

  28. Zheng, C.-J., Shao, C.-L., Chen, M., Niu, Z.-G., Zhao, D.-L., and Wang, C.-Y., Chem. Biodiversity, 2015, vol. 12, no. 9, pp. 1407–1414.

    Article  CAS  Google Scholar 

  29. Tsuda, M., Mugishima, T., Komatsu, K., Sone, T., Tanaka, M., Mikami, Y., and Kobayashi, J., J. Nat. Prod., 2003, vol. 66, no. 3, pp. 412–415.

    Article  CAS  Google Scholar 

  30. Trisuwan, K., Rukachaisirikul, V., Phongpaichit, S., Preedanon, S., and Sakayaroj, J., Arch. Pharm. Res., 2011, vol. 34, no. 5, pp. 709–714.

    Article  CAS  Google Scholar 

  31. Bhilabutra, W., Techowisan, T., Peberdy, J.F., and Lumyong, S., Res. J. Microbiol., 2007, vol. 2, no. 10, pp. 749–755.

    Article  CAS  Google Scholar 

  32. Perepogu, A.K., Raman, D., Murty, U.S.N., and Rao, V.J., Bioorg. Chem., 2009, vol. 37, no. 1, pp. 46–51.

    Article  CAS  Google Scholar 

  33. Jangili, P., Kashanna, J., Kumar, C.G., Poornachandra, Y., and Das, B., Bioorg. Med. Chem. Lett., 2014, vol. 24, no. 1, pp. 325–327.

    Article  CAS  Google Scholar 

  34. Sun, P., Lu, S., Ree, T.V., Krohn, K., Li, L., and Zhang, W., Curr. Med. Chem., 2012, vol. 19, no. 20, pp. 3417–3455.

    Article  CAS  Google Scholar 

  35. Liu, Q.-A., Zheng, J.-J., Gu, Y.-C., Wang, C.-Y., and Shao, C.-L., Stud. Nat. Prod. Chem., 2015, vol. 44, pp. 353–401.

    Article  Google Scholar 

  36. Berestetskii, A.O., Yuzikhin, O.S., Katkova, A.S., Dobrodumov, A.V., Sivogrivov, D.E., and Kolombet, L.V., Appl. Biochem. Microbiol., 2010, vol. 46, no. 1, pp. 84–88.

    Article  CAS  Google Scholar 

  37. Ellman, G.L., Courtney, K.D., Andres, V.Jr., and Feather-Stone, R.M., Biochem. Pharmacol., 1961, vol. 7, no. 1, pp. 88–95.

    Article  CAS  Google Scholar 

  38. Yang, Z.P. and Dettbarn, W.D., Biochem. Pharmacol., 1998, vol. 55, no. 8, pp. 1419–1426.

    Article  CAS  Google Scholar 

  39. Dospekhov, B.A., Metodika polevogo opyta (s osnovami statisticheskoi obrabotki rezul’tatov issledovanii) (Field Experiment Methodology (with the Basics of Statistical Processing of Research Results)), Moscow: Agropromizdat, 1985.

  40. Sudhakar, C., Reddy, P.R., Kumar, C.G., Sujitha, P., and Das, B., Eur. J. Org. Chem., 2012, vol. 2012, no. 6, pp. 1253–1258.

    Article  CAS  Google Scholar 

  41. Singh, B., Bhagat, J., Chadha, B.S., and Kaur, A., Biologia, 2014, vol. 69, no. 1, pp. 10–14.

    CAS  Google Scholar 

  42. Grossberg, G.T., Curr. Ther. Res. Clin. Exp., 2003, vol. 64, no. 4, pp. 216–235.

    Article  CAS  Google Scholar 

  43. Koitka, M., Hochel, J., Gieschen, H., and Borchert, H.H., J. Pharm. Biomed. Anal., 2010, vol. 51, no. 3, pp. 664–678.

    Article  CAS  Google Scholar 

  44. Čolović, M.B., Krstić, D.Z., Lazarević-Pašti, T.D., Bondžić, A.M., and Vasić, V.M., Curr. Neuropharmacol., 2013, vol. 11, no. 3, pp. 315–335.

    Article  Google Scholar 

  45. Rivero-Cruz, J.F., Macías, M., Cerda-García-Rojas, C.M., and Mata, R., J. Nat. Prod., 2003, vol. 66, no. 4, pp. 511–514.

    Article  CAS  Google Scholar 

Download references

Funding

The work was supported by the Russian Science Foundation (project no. 16-16-00085).

Author information

Authors and Affiliations

  1. All-Russian Research Institute of Plant Protection, 196608, St. Petersburg, Russia

    A. O. Berestetskiy & M. Yu. Belozyorova

  2. Research Institute for Hygiene, Occupational Pathology, and Human Ecology, 188663, Kuz’molovskiy, Leningrad Oblast, Russia

    D. S. Prokof’eva

Authors
  1. A. O. Berestetskiy
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Yu. Belozyorova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. S. Prokof’eva
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. O. Berestetskiy.

Ethics declarations

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

Additional information

Translated by T. Borisova

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Berestetskiy, A.O., Belozyorova, M.Y. & Prokof’eva, D.S. Effects of Substrate and Cultivation Duration on the Productivity, Biological Activity, and Chromatography Profiles of Extracts Obtained from Stagonospora cirsii S-47. Appl Biochem Microbiol 56, 78–90 (2020). https://doi.org/10.1134/S0003683820010032

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0003683820010032

Keywords:

Search

Navigation