Abstract
The lily-of-the-valley Convallaria (Asparagaceae) consists of three herbaceous perennial species. The plants are commonly found in northern hemisphere, and are best-known for their ornamental and pharmaceutical value. In order to assess the genetic structure, diversity and demographic history of Convallaria species, 19 novel microsatellite markers were developed based on transcriptome data of C. keiskei. Polymorphism and cross-amplification of the markers were tested in three populations of C. keiskei and one population each of C. majalis and C. montana. The transferability rate in two species was both 89.5%. The average number of alleles detected per locus was 7.7, 3.3 and 2.7 in C. keiskei, C. majalis and C. montana, respectively, and the polymorphism information content correspondingly varied from 0.067 to 0.730, from 0.071 to 0.637 and from 0.195 to 0.680 at the population level. The observed and expected heterozygosity ranged from 0.000 to 1.000 and from 0.000 to 0.833, respectively. Seven of the 19 loci showed significant deviation from Hardy–Weinberg equilibrium. The availability of these markers will provide a useful molecular tool for further population genetics, phylogeographic and breeding studies of Convallaria species.
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Acknowledgements
The project was supported by the National Natural Science Foundation of China (Grant Nos. 31700321, 31600183, 31400194), Zhejiang Provincial Public Welfare Technology and Application Research Project (Grant No. 2017C32044), Open Research Fund of Shanghai Key Laboratory of Plant Functional Genomics and Resources (Grant No. PFGR201703), and Science Foundation of Zhejiang Sci-Tech University (Grant No. 14042010-Y, 15042170-Y), 521 Distinguished Young Scientist Foundation of Zhejiang Sci-Tech University (Grant No. 11610132521509).
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PL, ZQ, and YW designed the research. XW, ZQ, PL and ML collected samples. XW and JW designed the primers. XZ and JW generated the data. JG and QL analyzed and interpreted the data. QL, JG, RW wrote the manuscript, and all the authors modified the manuscript.
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Lu, QX., Gao, J., Wu, JJ. et al. Development of 19 novel microsatellite markers of lily-of-the-valley (Convallaria, Asparagaceae) from transcriptome sequencing. Mol Biol Rep 47, 3041–3047 (2020). https://doi.org/10.1007/s11033-020-05376-9
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DOI: https://doi.org/10.1007/s11033-020-05376-9