Lysozyme/collagen multilayers layer-by-layer deposited nanofibers with enhanced biocompatibility and antibacterial activity

https://doi.org/10.1016/j.msec.2020.110868Get rights and content

Highlights

  • Lysozyme/collagen multilayers were LBL deposited on the surface of nanofibers

  • LBL structured nanofibers exhibited enhanced biocompatibility and antimicrobial properties

  • The coatings with 10 bilayers displayed the best biocompatibility

Abstract

Biological meshes have always posed a challenge in biological medicine, for which nanocomposites with enhanced biocompatibility and antibacterial activity may be beneficial. In this study, lysozyme (LY) and collagen (Col) were alternately deposited on silk fibroin (SF) and nylon 6 (N6) composite nanofibrous mats using a layer-by-layer (LBL) self-assembly technique. The mechanical properties, biocompatibility, and antibacterial activity of the LBL structured mats were characterized systematically to investigate the impact of the LBL process on the biological properties of SF/N6 nanofibrous mats. Our results showed that the effective deposition of LY and Col may affect the surface topography, mechanical properties, and wetting behavior of the SF/N6 nanofibrous mats. Moreover, LBL structured mats exhibited excellent biocompatibility and antibacterial properties. Among all the tested mats, those coated with 10 bilayers of LY and Col displayed the best biocompatibility, and relatively good mechanical and antibacterial properties. Thus, LBL structured mats, especially those with a 10 bilayer coating, are potentially valuable in clinical therapy for pelvic organ prolapse in the future.

Keywords

Lysozyme
Collagen
LBL deposition
Nanofibrous mats
Biomedical performance

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