Skip to main content
SpringerLink
Log in

Petroselinum sativum protects HepG2 cells from cytotoxicity and oxidative stress induced by hydrogen peroxide

  • Original Article
  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

A number of liver diseases are known to be caused by oxidative stress. Petroselinum sativum (P. sativum; parsley) is popular for its anti-inflammatory, antimicrobial, anticancer, antioxidant and antidiabetic activities. However, till date the hepatoprotective potential of chloroform extract of P. sativum (PSA) on hydrogen peroxide (H2O2) induced cytotoxicity and oxidative stress in human liver (HepG2) cells have not been studied. Therefore, this study was framed to evaluate whether the levels of hydrogen peroxide (H2O2) induced cytotoxicity and oxidative stress in HepG2 cells could be diminished by pretreating the cells with PSA. MTT assay, NRU assay, morphological alterations, glutathione (GSH) depletion, lipid peroxidation (LPO), ROS generation and loss of mitochondrial membrane potential (MMP) were assessed by using non-cytotoxic concentrations (5, 10 and 25 μg/mL) of PSA against H2O2 (0.25 mM) induced damage in HepG2 cells. The results demonstrated that pretreatment of HepG2 cells with PSA offered protective properties by lowering the LPO and ROS generation and elevating the cell viability, GSH and MMP levels. Together, these results suggest that PSA has the hepatoprotective effect on H2O2 induced cell death in HepG2 cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References:s

  1. Guan YS, He Q (2015) Plants consumption and liver health. Evid Complement Altern Med 824185:1–10

    Google Scholar 

  2. Madrigal-Santillán E, Madrigal-Bujaidar E, Álvarez-González I, Sumaya-Martínez MT, Gutiérrez-Salinas J, Bautista M, Morales-González Á, y González-Rubio MG, Aguilar-Faisal JL, Morales-González JA (2014) Review of natural products with hepatoprotective effects. World J Gastroenterol 20:14787–14804

    PubMed  PubMed Central  Google Scholar 

  3. Gu X, Manautou JE (2012) Molecular mechanisms underlying chemical liver injury. Expert Rev Mol Med 14:e4

    PubMed  PubMed Central  Google Scholar 

  4. Akinloye OA, Olaniyi MO (2011) Hepato-protective effect of Cajanus cajan on tissue defense system in D-galactosamine-induced hepatitis in rats. Turk J Biochem 36:237–241

    Google Scholar 

  5. Wang FS, Fan JG, Zhang Z, Gao B, Wang HY (2014) The global burden of liver disease: the major impact of China. Hepatology 60:2099–2108

    PubMed  PubMed Central  Google Scholar 

  6. Okaiyeto K, Nwodo UU, Mabinya LV, Okoh AI (2018) A review on some medicinal plants with hepatoprotective effects. Pharmacogn Rev 12:186–199

    CAS  Google Scholar 

  7. Sathesh KS, Ravi KB, Krishna MG (2009) Hepatoprotective effect of Trichosanthes cucumerina var cucumerina L. on carbon tetrachloride induced liver damage in rats. J Ethnopharmacol 123:347–350

    Google Scholar 

  8. Ajboye TO, Yakubu MT, Salau AK, Oladiji AT, Akanji MA, Okogun JI (2010) Antioxidant and drug detoxification potential of aqueous extract of Annona senegalensis leaves in carbon tetrachloride-induced hepatocellular damage. Pharm Biol 48:1361–1370

    PubMed  Google Scholar 

  9. Partap S, Tewari U, Sharma K, Jha KK (2014) Hepatoprotective activity of whole plant extract of Leptadenia pyrotechnica against paracetamol induced damage in rats. J Drug Deliv 4:36–39

    Google Scholar 

  10. Castán A, Navarro Y, Sarría L, Larrosa R, Serradilla M, Serrablo A (2017) Radiological diagnosis of hepatocellular carcinoma in non-cirrhotic patients. Hepatoma Res 3:1–17

    Google Scholar 

  11. Dutta S, Chakraborty AK, Dey P, Kar P, Guha P, Sen S, Kumar A, Sen A, Chaudhuri TK (2018) Amelioration of CCl4 induced liver injury in swiss albino mice by antioxidant rich leaf extract of Croton bonplandianus Baill. PLoS ONE 13:e0196411

    PubMed  PubMed Central  Google Scholar 

  12. Phaniendra A, Jestadi DB, Periyasamy L (2015) Free radicals: properties, sources, targets, and their implication in various diseases. Indian J Clin Biochem 30:11–26

    CAS  Google Scholar 

  13. Pandit A, Sachdeva T, Bafna P (2012) Drug-induced hepatotoxicity: a review. J Appl Pharm Sci 2:233–243

    Google Scholar 

  14. Dogan A, Dalar A, Sadullahoglu C, Battal A, Uzun Y, Celik I, Demirel K (2018) Investigation of the protective effects of horse mushroom (Agaricus arvensis Schaeff.) against carbon tetrachloride-induced oxidative stress in rats. Mol Biol Rep 45:787–797

    CAS  PubMed  Google Scholar 

  15. Siddiqui MA, Ali Z, Chittiboyina AG, Khan IA (2018) Hepatoprotective effect of steroidal glycosides from dioscorea villosa on hydrogen peroxide-induced hepatotoxicity in HepG2 cells. Front Pharmacol 9:797

    PubMed  PubMed Central  Google Scholar 

  16. El-Sayed AE, Aboulthana WM, El-Feky AM, Ibrahim NE, Seif MM (2018) Bio and phyto-chemical effect of Amphora coffeaeformis extract against hepatic injury induced by paracetamol in rats. Mol Biol Rep 45:2007–2023

    CAS  PubMed  Google Scholar 

  17. Bae WY, Kim HY, Park EH, Kim KT, Paik HD (2019) Improved in vitro antioxidant properties and hepatoprotective effects of a fermented Inula britannica extract on ethanol-damaged HepG2 cells. Mol Biol Rep 46:6053–6063

    CAS  PubMed  Google Scholar 

  18. Ajiboye TO, Ahmad FM, Daisi AO, Yahaya AA, Ibitoye OB, Muritala HF, Sunmonu TO (2017) Hepatoprotective potential of Phyllanthus muellarianus leaf extract: studies on hepatic, oxidative stress and inflammatory biomarkers. Pharm Biol 55:1662–1670

    PubMed  PubMed Central  Google Scholar 

  19. Alam J, Mujahid M, Badruddeen JY, Bagga P, Rahman MA (2017) Hepatoprotective potential of ethanolic extract of Aquilaria agallocha leaves against paracetamol induced hepatotoxicity in SD rats. J Tradit Complement Med 7:9–13

    PubMed  Google Scholar 

  20. Meera R, Devi P, Kameswari B, Madhumitha B, Merlin NJ (2009) Antioxidant and hepatoprotective activities of Ocimum basilicum linn. and Trigonella foenum-graecum linn. against H2O2 and CCL4 induced hepatotoxicity in goat liver. Indian J Exp Biol 47:584–590

    CAS  PubMed  Google Scholar 

  21. Rahim SM, Taha EM, Al-janabi MS, Al-douri BI, Simon KD, Mazlan AG (2014) Hepatoprotective effect of Cymbopogon citratus aqueous extract against hydrogen peroxide-induced liver injury in male rats. Afr J Tradit Complement Altern Med 11:447–451

    PubMed  PubMed Central  Google Scholar 

  22. Ghaffari H, Ghassam BJ, Prakash HS (2012) Hepatoprotective and cytoprotective properties of Hyptis suaveolens against oxidative stress–induced damage by CCl4 and H2O2. Asian Pac J Trop Med 5:868–874

    CAS  PubMed  Google Scholar 

  23. Al-Sheddi ES, Farshori NN, Al-Oqail MM, Musarrat J, Al-Khedhairy AA, Siddiqui MA (2016) Protective effect of Lepidium sativum seed extract against hydrogen peroxide-induced cytotoxicity and oxidative stress in human liver cells (HepG2). Pharm Biol 54:314–321

    PubMed  Google Scholar 

  24. Kreydiyyeh SI, Usta J, Kaouk I, Al-Sadi R (2001) The mechanism underlying the laxative properties of parsley extract. Phytomedicine 8:382–388

    CAS  PubMed  Google Scholar 

  25. Yanardağ R, Bolkent Ş, Tabakoğlu-Oğuz A, Özsoy-Saçan Ö (2003) Effects of Petroselinum crispum extract on pancreatic B cells and blood glucose of streptozotocin-induced diabetic rats. Biol Pharm Bull 26:1206–1210

    PubMed  Google Scholar 

  26. Ahmed NZ, El-Deib KM, Ahmed MM (2010) Studies on curcuma longa, chicorium intybus and Petroselinum sativum water extracts against cisplatin-induced toxicity in rats. J Am Sci 6:545–558

    Google Scholar 

  27. Wahba NM, Ahmed AS, Ebraheim ZZ (2010) Antimicrobial effects of pepper, parsley, and dill and their roles in the microbiological quality enhancement of traditional Egyptian Kareish cheese. Foodborne Pathog Dis 7:411–418

    CAS  PubMed  Google Scholar 

  28. Farshori NN, Al-Sheddi ES, Al-Oqail MM, Musarrat J, Al-Khedhairy AA, Siddiqui MA (2013) Anticancer activity of Petroselinum sativum seed extracts on MCF-7 human breast cancer cells. Asian Pac J Cancer Prev 14:5719–5723

    PubMed  Google Scholar 

  29. Siddiqui MA, Singh G, Kashyap MP, Khanna VK, Yadav S, Chandra D, Pant AB (2008) Influence of cytotoxic doses of 4-hydroxynonenal on selected neurotransmitter receptors in PC-12 cells. Toxicol In Vitro 22:1681–1688

    CAS  PubMed  Google Scholar 

  30. Siddiqui MA, Kashyap MP, Kumar V, Al-Khedhairy AA, Musarrat J, Pant AB (2010) Protective potential of trans-resveratrol against 4-hydroxynonenal induced damage in PC12 cells. Toxicol In Vitro 24:1592–1598

    CAS  PubMed  Google Scholar 

  31. Chandra D, Ramana KV, Wang L, Christensen BN, Bhatnagar A, Srivastava SK (2002) Inhibition of fiber cell globulization and hyperglycemiainduced lens opacification by aminopeptidase inhibitor bestatin. Invest Ophthalmol Vis Sci 43:2285–2292

    PubMed  Google Scholar 

  32. Buege JA, Aust SD (1978) Microsomal lipid peroxidation. Methods Enzymol 52:302–310

    CAS  PubMed  Google Scholar 

  33. Siddiqui MA, Ahmad J, Farshori NN, Saquib Q, Jahan S, Kashyap MP, Ahamed M, Musarrat J, Al-Khedhairy AA (2013) Rotenone-induced oxidative stress and apoptosis in human liver HepG2 cells. Mol Cell Biochem 384:59–69

    CAS  PubMed  Google Scholar 

  34. Zhang Y, Jiang L, Jiang L, Geng C, Li L, Shao J, Zhong L (2011) Possible involvement of oxidative stress in potassium bromate-induced genotoxicity in human HepG2 cells. Chem Biol Interact 189:186–191

    CAS  PubMed  Google Scholar 

  35. Jiang J, Yu S, Jiang Z, Liang C, Yu W, Li J, Du X, Wang H, Gao X, Wang X (2014) N-acetyl-serotonin protects HepG2 cells from oxidative stress injury induced by hydrogen peroxide. Oxid Med Cell Long 310504:1–15

    Google Scholar 

  36. Sattayasai J, Chaonapan P, Arkaravichie T, Soi-ampornkul R, Junnu S, Charoensilp P, Samer J, Jantaravinid J, Masaratana P, Suktitipat B, Manissorn J (2013) Protective effects of Mangosteen extract on H2O2-induced cytotoxicity in SK-NSH cells and scopolamine-induced memory impairment in mice. PLoS ONE 8:e85053

    PubMed  PubMed Central  Google Scholar 

  37. Pareek A, Godavarthi A, Issarani R, Nagori BP (2013) Antioxidant and hepatoprotective activity of Fagonia schweinfurthii (Hadidi) Hadidi extract in carbon tetrachloride induced hepatotoxicity in HepG2 cell line and rats. J Ethnopharmacol 150:973–981

    PubMed  Google Scholar 

  38. Krithika R, Mohankumar R, Verma RJ, Shrivastav PS, Mohamad IL, Gunasekaran P, Narasimhan S (2009) Isolation, characterization and antioxidative effect of phyllanthin against CCl4-induced toxicity in HepG2 cell line. Chem Biol Interact 181:351–358

    CAS  PubMed  Google Scholar 

  39. Joshi BC, Prakash A, Kalia AN (2015) Hepatoprotective potential of antioxidant potent fraction from Urtica dioica Linn. (whole plant) in CCl4 challenged rats. Toxicol Rep 2:1101–1110

    CAS  PubMed  PubMed Central  Google Scholar 

  40. Farshori NN, Al-Sheddi ES, Al-Oqail MM, Hassan WH, Al-Khedhairy AA, Musarrat J, Siddiqui MA (2015) Hepatoprotective potential of Lavandula coronopifolia extracts against ethanol induced oxidative stress-mediated cytotoxicity in HepG2 cells. Toxicol Ind Health 8:727–737

    Google Scholar 

  41. Hiraganahalli BD, Chinampudur VC, Dethe S, Mundkinajeddu D, Pandre MK, Balachandran J, Agarwal A (2012) Hepatoprotective and antioxidant activity of standardized herbal extracts. Phcog Mag 8:116–123

    PubMed  Google Scholar 

  42. Rashid U, Khan MR, Sajid M (2016) Hepatoprotective potential of Fagonia Olivieri DC against acetaminophen induced toxicity in rat. BMC Complement Altern Med 16:449

    PubMed  PubMed Central  Google Scholar 

  43. Xia T, Yao J, Zhang J, Zheng Y, Song J, Wang M (2017) Protective effects of Shanxi aged vinegar against hydrogen peroxide-induced oxidative damage in LO2 cells through Nrf2-mediated antioxidant responses. RSC Adv 7:17377–17386

    CAS  Google Scholar 

  44. Wahid A, Hamed AN, Eltahir HM, Abouzied MM (2016) Hepatoprotective activity of ethanolic extract of Salix subserrata against CCl 4-induced chronic hepatotoxicity in rats. BMC Complement Altern Med 16:263

    PubMed  PubMed Central  Google Scholar 

  45. Niki E (2009) Lipid peroxidation: physiological levels and dual biological effects. Free Radic Biol Med 47:469–484

    CAS  PubMed  Google Scholar 

  46. Razali N, Junit SM, Ariffin A, Ramli NS, Aziz AA (2015) Polyphenols from the extract and fraction of T. indica seeds protected HepG2 cells against oxidative stress. BMC Complement Altern Med 15:438

    PubMed  PubMed Central  Google Scholar 

  47. Kong KW, Mat-Junit S, Aminudin N, Hassan FA, Ismail A, Aziz AA (2016) Protective effects of the extracts of Barringtonia racemosa shoots against oxidative damage in HepG2 cells. PeerJ 4:e1628

    PubMed  PubMed Central  Google Scholar 

  48. Nakchat O, Nalinratana N, Meksuriyen D, Pongsamart S (2014) Tamarind seed coat extract restores reactive oxygen species through attenuation of glutathione level and antioxidant enzyme expression in human skin fibroblasts in response to oxidative stress. Asian Pac J Trop Biomed 4:379–385

    CAS  PubMed  PubMed Central  Google Scholar 

  49. Wang CC, Fang KM, Yang CS, Tzeng SF (2009) Reactive oxygen species-induced cell death of rat primary astrocytes through mitochondria-mediated mechanism. J Cell Biochem 107:933–943

    CAS  PubMed  Google Scholar 

  50. Su M, Yu T, Zhang H, Wu Y, Wang X, Li G (2016) The antiapoptosis effect of glycyrrhizate on HepG2 cells induced by hydrogen peroxide. Oxid Med Cell Long 6849758:1–9

    Google Scholar 

  51. Grauzdytė D, Pukalskas A, Viranaicken W, El Kalamouni C, Venskutonis PR (2018) Protective effects of Phyllanthus phillyreifolius extracts against hydrogen peroxide induced oxidative stress in HEK293 cells. PLoS ONE 13:e0207672

    PubMed  PubMed Central  Google Scholar 

  52. Hengartner MO (2000) The biochemistry of apoptosis. Nature 407:770–776

    CAS  PubMed  Google Scholar 

  53. Zhang Y, Wang X, Baranov SV, Zhu S, Huang Z, Fellows-Mayle W, Jiang J, Day AL, Kristal BS, Friedlander RM (2011) Dipyrone inhibits neuronal cell death and diminishes hypoxic/ischemic brain injury. Neurosurgery 69:942–956

    PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

The authors are grateful to the Deanship of Scientific Research, King Saud University for funding through Vice Deanship of Scientific Research Chairs.

Author information

Authors and Affiliations

  1. Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia

    Mai M. Al-Oqail, Nida N. Farshori, Ebtesam S. Al-Sheddi & Shaza M. Al-Massarani

  2. DNA Research Chair, Zoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia

    Maqsood A. Siddiqui

  3. Zoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia

    Maqsood A. Siddiqui & Abdulaziz A. Al-Khedhairy

Authors
  1. Mai M. Al-Oqail
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nida N. Farshori
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ebtesam S. Al-Sheddi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shaza M. Al-Massarani
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Maqsood A. Siddiqui
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Abdulaziz A. Al-Khedhairy
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Maqsood A. Siddiqui.

Ethics declarations

Conflicts of interest

The authors declare that they have no conflicts of interest.

Ethical approval

This paper does not contain any studies with human or animals.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Al-Oqail, M.M., Farshori, N.N., Al-Sheddi, E.S. et al. Petroselinum sativum protects HepG2 cells from cytotoxicity and oxidative stress induced by hydrogen peroxide. Mol Biol Rep 47, 2771–2780 (2020). https://doi.org/10.1007/s11033-020-05380-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-020-05380-z

Keywords

Search

Navigation