Abstract
This work identified a novel rhabdo-like virus in a Chinese black cutworm (Agrotis ipsilon), which we tentatively named “Agrotis ipsilon virus” (AIpsV). The complete genome of AIpsV is 15,454 nucleotides in length and contains seven open reading frames, collectively encoding more than 160 amino acids. The AIpsV genome is predicted to encode three structural proteins, nucleoprotein (N), glycoprotein (G), and large polymerase protein (L), and four unknown proteins. Phylogenetic analysis revealed that the AIpsV clusters with Wuhan ant virus and Hubei rhabdo-like virus 1 within the rhabdo-like virus clade. The level of expression of AIpsV genes was found to be higher in the pupal and adult stages than in the egg and larval stages.
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Funding
This work was supported by the National Basic Research Program of China (2015CB755703), the National Natural Science Foundation of China (31702057 and 31601897), and Fund for Distinguished Young Scholars from Henan Academy of Agricultural Sciences (Grant no. 2020JQ05).
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Fig. S1 Maximum-likelihood phylogenetic tree of members of the order Mononegavirales and chuviruses, based on RdRp protein sequences. The clade corresponding to the family Rhabdoviridae is highlighted. Numbers near nodes indicate ultrafast bootstrap values. The bar indicates the estimated number of substitutions per site
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Chen, S., Li, H., Yuan, G. et al. Complete genome sequence of a novel rhabdo-like virus from the Chinese black cutworm Agrotis ipsilon (Lepidoptera: Noctuidae). Arch Virol 165, 989–991 (2020). https://doi.org/10.1007/s00705-020-04559-w
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DOI: https://doi.org/10.1007/s00705-020-04559-w