Abstract
Silver nanoparticles (AgNPs) are one of the new cancer treatment tools due to their unique properties that enhance potential therapeutic efficacy. In this study, we describe the extracellular biosynthesis and anticancer activity of AgNPs using the Anabaena flos-aquae biomass extract as reducing agent. The formation of dark-brown \(\hbox {AgNO}_{{3}}\)/extract solution confirmed the reduction of silver ions into AgNPs. In addition, the ultraviolet–visible spectroscopy showed the surface plasmon peak at 425 nm as characteristic peak for AgNPs. Transmission electron microscopy and scanning electron microscopy showed highly stable and mostly spherical AgNPs with average size of 5–25 nm. Fourier transform infrared spectral analysis confirmed the presence of biomolecules in the extract involved in the reduction and stabilization of AgNPs. In vitro, study of anticancer and cytotoxic effect of AgNPs and extract against T47D cell lines by MTT assay and flow cytometry confirmed the anti-proliferation potential of AgNPs against breast cancer cells. In conclusion, our results revealed that Anabaena can be used as a good organism for biologically synthesis of AgNPs and confirmed the potent therapeutic value of these nanoparticles as anticancer drugs.
Similar content being viewed by others
References
Sharma V K, Yngard R A and Lin Y 2009 Adv. Colloid Interface Sci. 145 83
Desireddy A, Conn B E, Guo J, Yoon B, Barnett R N and Monahan B M 2013 Nature 501 399
Pulit-Prociak J and Banach M 2016 Open Chem. 14 76
Le Ouay B and Stellacci F 2015 Nano Today 10 339
Raghunandan D, Ravishankar B, Sharanbasava G, Mahesh D B, Harsoor V, Yalagatti M S et al 2011 Cancer Nanotechnol. 2 57
Gonzalez L, Lison D and Kirsch-Volders M 2008 Nanotoxicology 2 252
Ray P C, Yu H and Fu P P 2009 J. Environ. Sci. Health. C: Environ. Carcinog. Ecotoxicol. Rev. 27 1
Abdal Dayem A, Hossain M K, Lee S B, Kim K, Saha S K and Yang G M 2017 Int. J. Mol. Sci. 18 120
Iravani S, Korbekandi H, Mirmohammadi S V and Zolfaghari B 2014 Res. Pharm. Sci. 9 385
Dimitrijevic N M, Bartels D, Jonah C D, Takahashi K and Rajh T 2001 J. Phys. Chem. 105 954
Nasretdinova G R, Fazleeva R R, Mukhitova R K, Nizameev I R, Kadirov M K and Ziganshina A Y 2015 Electrochem. Commun. 50 69
Callegari M, Tonti D and Chergui M 2003 Nano Lett. 3 1565
Junejo Y and Sirajuddin A 2014 J. Inorg. Organomet. Polym. Mater. 24 401
Panda K K, Achary V M, Krishnaveni R, Padhi B K, Sarangi S N, Sahu S N et al 2011 Toxicol. In Vitro 25 1097
Schneidewind H, Schuler T, Strelau K K, Weber K, Cialla D and Diegel M 2012 Beilstein J. Nanotechnol. 3 404
Iravani S 2011 Green Chem. 13 2638
Balaji D S, Basavaraja S, Deshpande R, Mahesh D B, Prabhakar B K and Venkataraman A 2009 Colloids Surf. B: Biointerfaces 68 88
Sudha S S, Rajamanickam K and Rengaramanujam J 2013 Ind. J. Exp. Biol. 52 393
Lengke M F and Fleet M E 2007 Langmuir 23 2694
Yu Y, You L, Liu D, Hollinshead W, Tang Y J and Zhang F 2013 Mar. Drugs 11 2894
Patel V, Berthold D, Puranik P and Gantar M 2015 Biotechnol. Rep. 5 112
Tao Z, Shi A, Lu C, Song T, Zhang Z and Zhao J 2015 Cell Biochem. Biophys. 72 333
Sanpui P, Chattopadhyay A and Ghosh S S 2011 ACS Appl. Mater. Interfaces 3 218
Canovi M, Lucchetti J, Stravalaci M, Re F, Moscatelli D, Bigini P et al 2012 Sensors 12 16420
Jeyaraj M, Renganathan A, Sathishkumar G, Ganapathi A and Premkumar K 2015 RSC Adv. 3 2159
Zhang X F, Liu Z G, Shen W and Gurunathan S 2016 Int. J. Mol. Sci. 17 1534
Sankar R, Karthik A, Prabu A, Karthik S, Shivashankari K S and Ravikumar V 2013 Colloids Surf. B: Biointerfaces 108 80
Reddy N J, Vali D N and Rani M 2014 Mater. Sci. Eng. 34 115
Vinardell M P and Mitjans M 2015 Nano 5 1004
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ebrahimzadeh, Z., Salehzadeh, A., Naeemi, A.S. et al. Silver nanoparticles biosynthesized by Anabaena flos-aquae enhance the apoptosis in breast cancer cell line. Bull Mater Sci 43, 92 (2020). https://doi.org/10.1007/s12034-020-2064-1
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s12034-020-2064-1