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Effects of Actin Cytoskeleton Disruption on Electroporation In Vitro

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Abstract

The role of actin fibers in cellular responses to external electric pulses is not clear yet. In this study, we utilized the blocker of actin polymerization, cytochalasin D (cytoD), and investigated its effects on the electropore generation. Eight 100 μs electric pulses of sub-kilovolt per centimeter voltage with 100 ms intervals were applied to adhered cells in vitro, and the membrane permeability was quantified using membrane-impermeable propidium iodide (PI) dye. With cytoD application, the transfer of PI dye decreased significantly in all the applied voltages. At the same time, the roughness of cells increased, the membrane stiffness decreased, and the transmembrane resting potential decreased. Our result supports that actin fibers have clear effects on electroporation through modulating membrane properties including transmembrane resting potential.

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Funding

This work was supported by the Ministry of SMEs and Startups (grant numbers S2459501) (Korea) and supported by Kwangwoon University 2018.

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  1. Hong Bae Kim and Seho Lee contributed equally to this work.

Authors and Affiliations

  1. Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul, 08826, Republic of Korea

    Hong Bae Kim & Jong Hoon Chung

  2. Department of Brain and Cognitive Engineering, Korea University, Seoul, 02841, Republic of Korea

    Seho Lee

  3. Medical Engineering Research Center, The Standard Co. Ltd., Gyeonggi-do, 15880, Republic of Korea

    Seong Nam Kim

  4. Department of Radiology, Seoul National University Cancer Research Institute, SNU-SMG Boramae Medical Center, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea

    Chang Kyu Sung

  5. Department of Biological and Electrical Physics, Kwangwoon University, Seoul, 01897, Republic of Korea

    Ku Youn Baik

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  1. Hong Bae Kim
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Kim, H.B., Lee, S., Chung, J.H. et al. Effects of Actin Cytoskeleton Disruption on Electroporation In Vitro. Appl Biochem Biotechnol 191, 1545–1561 (2020). https://doi.org/10.1007/s12010-020-03271-4

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