Abstract
A previously fully validated method using high-performance liquid chromatography with diode array detection (HPLC-DAD) was applied to determine 12 bioactive phenolic compounds in 9 different fruits consumed in Salvador, Bahia, Brazil. A central composite design (CCD) was employed to investigate the effects of independent variables in the extraction method for spectrophotometric determinations. The model showed a good correlation between the predicted and experimental values. The results indicate that the fruits are rich in polyphenols, mainly ellagic acid, vanillic acid, rutin and quercetin. Total polyphenol content (TPC), total flavonoid content (TFC), total anthocyanin content (TAC), and antioxidant capacity were also determined by DPPH assay and can be related to the diverse range of phenolics detected. Kohonen Self- Organizing Map (SOM) Artificial Neural Network was applied for more insights about cluster separation and the influence of each variable considering one of its main characteristics related to the treatment of non-linear data. A true classification model partial least squares-discriminant analysis (PLS-DA) and multilayer perceptron (MLP) artificial neural network were applied to the current samples, and while MLP proved to be a suitable technique for the classification of different fruits evaluated in this study, PLS-DA obtained a very large experimental error due to the similarities of the experimental data and also due to the existence of non-linear relationships between the variables.
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Funding
The authors are grateful to the Brazilian Agency, to the Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Programa de Apoio a Núcleos de Excelência (PRONEX) by the grants and fellowships. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001.
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Bruna Rosa da Silva Santos declares that she has no conflict of interest. Lucas Almir Cavalcante Minho that he has no conflict of interest. Emmanuelle Ferreira Requião Silva that she has no conflict of interest. Maria Celeste da Silva Sauthier that she has no conflict of interest. Jamile da Cruz Caldas that she has no conflict of interest. Erik Galvão Paranhos da Silva that he has no conflict of interest. Débora de Andrade Santana that she has no conflict of interest. Walter Nei Lopes dos Santos that she has no conflict of interest.
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da Silva Santos, B.R., Minho, L.A.C., Silva, E.F.R. et al. Chemometric Tools Applied to Evaluation of Fruit Bioactive Compounds Extraction. Food Anal. Methods 13, 1176–1189 (2020). https://doi.org/10.1007/s12161-020-01728-0
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DOI: https://doi.org/10.1007/s12161-020-01728-0