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Efficacy of mouthrinses with bovine milk and milk protein isolates to accumulate casein in the in situ pellicle

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Abstract

Objectives

The adsorption of bovine milk caseins on the tooth surface might have a positive impact on the prevention of dental diseases. Therefore, the present study aimed to investigate the efficacy of mouthrinses with different types of bovine milk and milk protein isolates to accumulate caseins in the pellicle.

Materials/methods

An indirect enzyme-linked immunosorbent assay (ELISA) was established to quantify the amount of caseins adsorbed into the in situ pellicle. In situ pellicle samples were collected from 2 volunteers on ceramic specimens (A = 8 cm2). After 10 min of pellicle formation, different types of bovine milk, 3% micellar casein in synthetic milk ultrafiltrate (SMUF) or 3% non-micellar caseinate in SMUF, were used as mouthrinses for 10 min. The pellicle material was harvested after 30 min in situ and examined for caseins by the indirect ELISA. Selected pellicle samples were subjected to TEM analysis.

Results

All mouthrinses accumulated caseins in the in situ pellicle (2.0 ± 0.7–20 ± 1.7 μg/ml) that, under native conditions, expressed no casein signal. Micellar protein association increased the adsorption of casein into the pellicle. Milk homogenization also had an influence on the casein accumulation in the pellicle. TEM analysis confirmed the integration of micellar casein into the pellicle.

Conclusion

The mouthrinses altered the protein composition and the ultrastructure of the in situ pellicle to a different extent: bovine milk with 3.8% fat content and 3% micellar casein in SMUF being particularly effective.

Clinical relevance

The study provides interesting perspectives for innovative prevention strategies in dentistry.

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Funding

This study has been funded by the German Research Foundation (HA 2718/20-1; KE 2186/1-1).

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Authors and Affiliations

  1. Clinic of Operative and Pediatric Dentistry, Medical Faculty Carl Gustav Carus, TU Dresden, Fetscherstr. 74, 01307, Dresden, Germany

    A. Kensche, S. Pötschke & C. Hannig

  2. Chair of Food Chemistry, TU Dresden, Dresden, Germany

    A. Dürasch & T. Henle

  3. Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, University Hospital, Saarland University, Building 73, 66421, Homburg, Saarland, Germany

    M. Hannig

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  1. A. Kensche
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  2. S. Pötschke
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  3. C. Hannig
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  4. A. Dürasch
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  5. T. Henle
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Correspondence to A. Kensche.

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The authors declare that they have no conflict of interest.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. The performed study design was reviewed and approved by the ethics committee of the Medical Faculty, Technische Universität Dresden, Germany (vote: EK 147052013).

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A. Kensche and S. Pötschke shared first authorship

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Kensche, A., Pötschke, S., Hannig, C. et al. Efficacy of mouthrinses with bovine milk and milk protein isolates to accumulate casein in the in situ pellicle. Clin Oral Invest 24, 3871–3880 (2020). https://doi.org/10.1007/s00784-020-03253-0

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  • DOI: https://doi.org/10.1007/s00784-020-03253-0

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