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Characterization of Pingliang xiaoheidou (ZDD 11047), a soybean variety with resistance to soybean cyst nematode Heterodera glycines

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Key message

A novel QTL (qSCN-PL10) for SCN resistance and related candidate genes were identified in the soybean variety Pingliang xiaoheidou, and plant basal immunity seems to contribute to the SCN resistance.

Abstract

Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) is one of the most devastating soybean pests worldwide. The development of host plant resistance represents an effective strategy to control SCN. However, owing to the lack of diversity of resistance genes in soybean varieties, further investigation is necessary to identify new SCN resistance genes. By analyzing the resistance phenotypes of soybean variety Pingliang xiaoheidou (Pingliang, ZDD 11047), we found that it exhibited the different resistance phenotypes from PI 88788 and Peking varieties. Because Pingliang variety contains the Rhg1-a (low copy) haplotype and lacks the resistant Rhg4 haplotype, novel quantitative trait locus might account for their SCN resistance. After sequencing parental lines (Magellan and Pingliang) and 200 F2:3 progenies, a high-density genetic map was constructed using the specific length amplified fragment sequencing method and qSCN-PL10 was identified as a novel locus for SCN resistance. Candidate genes were predicted by RNA sequencing (RNA-seq) in the qSCN-PL10 locus region. The RNA-seq analysis performed also indicated that plant basal immunity plays an important role in the resistance of Pingliang to SCN. These results lay a foundation for the use of marker-assisted breeding to enhance the resistance to SCN.

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Data availability

RNA-Seq‘s raw data has been published at WEBLINK https://www.ncbi.nlm.nih.gov/bioproject/579241 with BioProject ID PRJNA579241.

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Acknowledgements

This work was supported by the National Key R&D Program of China (2016YFD0100603), the Major project of Research and Development of Applied Technology in Heilongjiang Province (GA18B101), the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences, the National Natural Science Foundation of China (31501334 and 31522042).

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  1. Wei Guo and Jing S. Chen have contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, 430062, Hubei, China

    Wei Guo, Feng Zhang, Hai F. Chen, Chan J. Zhang, Li M. Chen, Song L. Yuan, Rong Li, Dong Cao, Qing N. Hao, Shui L. Chen, Zhi H. Shan, Zhong L. Yang, Xiao J. Zhang, De Z. Qiu, Qing B. You, Wen J. Dai, Xin A. Zhou & Xin J. Shen

  2. Daqing Branch of the Heilongjiang Academy of Agricultural Sciences, Daqing, 163316, Heilongjiang, China

    Jing S. Chen & Ze Y. Li

  3. Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Zhengzhou, 450002, Henan, China

    Yong Q. Jiao

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  1. Wei Guo
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Contributions

WG, XJS, JSC, and YQJ designed the study; WG, JSC, FZ, CJZ, and HFC performed the QTL and genomic qPCR analyses; LMC, SLY, RL, ZYL, WJD, and JSC performed the nematode assays; SLC, ZHS, ZLY, XJZ, DZQ, QBY, and XJS performed the qRT-PCR analysis; DC, QNH, WG, and JSC analyzed the data; WG, XJS, XAZ, and JSC wrote the paper; WG, XJS, and YQJ coordinated the study. All authors have read and approved the manuscript for submission.

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Correspondence to Wei Guo, Xin J. Shen or Yong Q. Jiao.

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Guo, W., Chen, J.S., Zhang, F. et al. Characterization of Pingliang xiaoheidou (ZDD 11047), a soybean variety with resistance to soybean cyst nematode Heterodera glycines. Plant Mol Biol 103, 253–267 (2020). https://doi.org/10.1007/s11103-020-00990-4

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