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A nucleoside diphosphate kinase gene OsNDPK4 is involved in root development and defense responses in rice (Oryza sativa L.)

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Abstract

Main conclusion

Dysfunctional mutation of OsNDPK4 resulted in severe defects in root development of rice. However, the resistance of Osndpk4 against bacterial blight was significantly enhanced.

Abstract

Nucleoside diphosphate kinases (NDPKs) are an evolutionarily conserved family of important enzymes balancing the energy currency nucleoside triphosphates by catalyzing the transfer of their phosphate groups. The aim of this study was to elucidate the function of OsNDPK4 in rice. A dysfunctional rice mutant was employed to characterize the function of OsNDPK4. Its expression and subcellular localization were examined. The transcriptomic change in roots of Osndpk4 was analyzed by RNA-seq. The rice mutant Osndpk4 showed severe defects in root development from the early seedling stage. Further analysis revealed that meristematic activity and cell elongation were significantly inhibited in primary roots of Osndpk4, together with reduced accumulation of reactive oxygen species (ROS). Map-based cloning identified that the mutation occurred in the OsNDPK4 gene. OsNDPK4 was found to be expressed in a variety of tissues throughout the plant and OsNDPK4 was located in the cytosol. Osndpk4 showed enhanced resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo) and up-regulation of pathogenesis-related marker genes. In addition, transcriptomic analysis showed that OsNDPK4 was significantly associated with a number of biological processes, including translation, protein modification, metabolism, biotic stress response, etc. Detailed analysis revealed that the dysfunction of OsNDPK4 might reorchestrate energy homeostasis and hormone metabolism and signalling, resulting in repression of translation, DNA replication and cell cycle progression, and priming of biotic stress defense. Our results demonstrate that OsNDPK4 plays important roles in energy homeostasis, development process, and defense responses in rice.

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Abbreviations

DEGs:

Differentially expressed genes

GUS:

β-Glucuronidase

KEGG:

Kyoto Encyclopedia of Genes and Genomes

NDPK:

Nucleoside diphosphate kinase

PR:

Pathogenesis related

ROS:

Reactive oxygen species

WT:

Wild type

Xoo :

Xanthomonas oryzae Pv. oryzae

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Acknowledgements

This work was supported by the National Natural Science Foundation of China [Grant Numbers 31300246, 31371595], the Zhejiang Provincial Natural Science Foundation of China [Grant Numbers LY17C020002], the Natural Science Foundation of Ningbo [Grant Number 2019A610413] and K. C. Wong Magna Fund in Ningbo University.

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  1. Jin Ye and Wona Ding contributed equally to this paper.

Authors and Affiliations

  1. College of Science and Technology, Ningbo University, Ningbo, 315211, People’s Republic of China

    Jin Ye, Wona Ding, Yujie Chen, Xinni Zhu, Jiutong Sun, Wenjuan Zheng & Shihua Zhu

  2. School of Marine Sciences, Ningbo University, Ningbo, 315211, People’s Republic of China

    Jin Ye

  3. Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, China

    Botao Zhang

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  1. Jin Ye
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Ye, J., Ding, W., Chen, Y. et al. A nucleoside diphosphate kinase gene OsNDPK4 is involved in root development and defense responses in rice (Oryza sativa L.). Planta 251, 77 (2020). https://doi.org/10.1007/s00425-020-03355-9

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