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Genome-wide analysis of laccase genes in moso bamboo highlights PeLAC10 involved in lignin biosynthesis and in response to abiotic stresses

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Abstract

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Twenty-three PeLACs have been identified in moso bamboo, overexpression of PeLAC10 increases the lignin content and confers drought and phenolic acid tolerance in transgenic Arabidopsis.

Abstract

Laccases (LACs) have multifunction involved in the processes of cell elongation, lignification and stress response in plants. However, the function of laccases in bamboo remain unclear. Here, a total of 23 laccase genes (PeLAC1–PeLAC23) were identified in moso bamboo (Phyllostachys edulis). The diverse gene structure and expression pattern of PeLACs suggested that their function should be spatiotemporal and complicated, which was supported by the expression profiles in different tissues of moso bamboo. Eighteen PeLACs were identified as the targets of ped-miR397. The putative ped-miR397-binding site in the coding region of PeLAC10 was further confirmed by RLM-5′ RACE, indicating that PeLAC10 was regulated by ped-miR397 after transcription. With the increasing shoot height, the expression abundance of PeLAC10 was up-regulated and reached the maximum in 15 cm shoots, while that of ped-miR397 was relative lower and showed the minimum in 15 cm shoots. PeLAC10 was up-regulated obviously under both ABA (100 μmol L–1) and NaCl (400 mmol L–1) treatments, and it was down-regulated under the GA3 (100 μmol L–1) treatment. The transgenic Arabidopsis plants over-expressing PeLAC10 became slightly smaller and their petioles were shorter than those of Col-0. However, they had a stronger capacity in resistance to phenolic acids and drought besides higher lignin content in stems. These results indicated that overexpression of PeLAC10 was helpful to increase the content of lignin in transgenic Arabidopsis and improve the adaptability to phenolic acid and drought stresses.

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Abbreviations

4CL:

4-(Hydroxy) cinnamoyl CoA ligase

C3H:

p-coumarate 3-hydroxylase

C4H:

Cinnamate 4-hydroxylase

CAD:

Cinnamyl alcohol dehydrogenase

CAT:

Catalase

CCoAOMT:

Caffeoyl CoA O-methyltransferase

CCR:

Cinnamoyl CoA reductase

COMT:

Caffeic acid/5-hydroxyferulic acid O-methyltransferase

F5H:

Ferulate 5-hydroxylase

LAC:

Laccase

MDA:

Malondialdehyde

ORF:

Open reading frame

PAL:

Phenylalanine ammonia-lyase

POD:

Peroxidase

qRT-PCR:

Quantitative real-time polymerase chain reaction

RLM-5′ RACE:

RNA ligase mediated 5′ rapid amplification of cDNA ends

ROS:

Reactive oxygen species

RT-PCR:

Reverse transcription PCR

SOD:

Superoxide dismutase

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Acknowledgements

This work was supported by the Special Fund for Fundamental Scientific Research on Professional Work Supported by the International Center for Bamboo and Rattan (No. 1632019008) and the Special Fund for Forest Scientific Research in the Public Welfare from State Forestry Administration of China (No. 201504106).

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  1. Lichao Li and Kebin Yang these authors contributed equally to this work.

Authors and Affiliations

  1. National Forestry and Grassland Administration, Beijing Key Open Laboratory On the Science and Technology of Bamboo and Rattan, Institute of Gene Science and Industrialization for Bamboo and Rattan Resources, International Center for Bamboo and Rattan, Beijing, 100102, China

    Lichao Li, Kebin Yang, Sining Wang, Chenglei Zhu & Zhimin Gao

  2. Jiangxi Academy of Forestry, Nanchang, 330013, China

    Yongfeng Lou

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  1. Lichao Li
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  2. Kebin Yang
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Contributions

ZMG and LCL designed the experiments; LCL and KBY performed experiments; LCL and KBY analyzed data; SNW, LYF, and CLZ contributed reagents/materials/analysis tools; ZMG and LCL wrote the paper. All authors read and approved the final manuscript.

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Correspondence to Zhimin Gao.

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Communicated by Hiroyasu Ebinuma.

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Li, L., Yang, K., Wang, S. et al. Genome-wide analysis of laccase genes in moso bamboo highlights PeLAC10 involved in lignin biosynthesis and in response to abiotic stresses. Plant Cell Rep 39, 751–763 (2020). https://doi.org/10.1007/s00299-020-02528-w

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