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Genome-wide identification of the NPR1-like gene family in Brassica napus and functional characterization of BnaNPR1 in resistance to Sclerotinia sclerotiorum

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Abstract

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The BnaNPR1-like gene family was identified in B. napus, and it was revealed that repression of BnaNPR1 significantly reduces resistance to S. sclerotiorum, intensifies ROS accumulation, and changes the expression of genes associated with SA and JA/ET signaling in response to this pathogen.

Abstract

The NONEXPRESSOR OF PATHOGENESIS-RELATED GENES1 (NPR1) and related NPR1-like genes play an important role in regulating plant defense. Oilseed rape (Brassica napus L.) is an important oilseed crop; however, little is known about the B. napus (Bna) NPR1-like gene family. Here, a total of 19 BnaNPR1-like genes were identified in the B. napus genome, and then named according to their respective best match in Arabidopsis thaliana (At), which led to the determination of B. napus homologs of every AtNPR1-like gene. Analysis of important protein domains and functional motifs indicated the conservation and variation among these homologs. Phylogenetic analysis of these BnaNPR1-like proteins and their Arabidopsis homologs revealed six distinct sub-clades, consequently indicating that their name classification totally conformed to their phylogenetic relationships. Further, B. napus transcriptomic data showed that the expression of three BnaNPR1s was significantly down-regulated in response to infection with Sclerotinia sclerotiorum, the most important pathogen of this crop, whereas BnaNPR2/3/4/5/6s did not show the expression differences in general. Further, we generated B. napus BnaNPR1-RNAi lines to interpret the effect of the down-regulated expression of BnaNPR1s on resistance to S. sclerotiorum. The results showed that BnaNPR1-RNAi significantly decreased this resistance. Further experiments revealed that BnaNPR1-RNAi intensified ROS production and changed defense responses in the interaction of plants with this pathogen. These results indicated that S. sclerotiorum might use BnaNPR1 to regulate specific physiological processes of B. napus, such as ROS production and SA defense response, for the infection.

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Acknowledgements

This work was supported by National Natural Science Foundation of China (no. 31771836) and National Key Research and Development Program of China (2018YFD0201003). The authors declare no conflict of interest.

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  1. Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang, 212013, People’s Republic of China

    Zheng Wang, Lu-Yue Ma, Xiao Li, Feng-Yun Zhao, Rehman Sarwar, Jun Cao, Yu-Long Li, Li-Na Ding, Ke-Ming Zhu, Yan-Hua Yang & Xiao-Li Tan

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299_2020_2525_MOESM4_ESM.pdf

Supplementary File S4 The protein sequence comparison of BnaNPR1-like genes and their Arabidopsis homologs. The amino acid sequences of 19 identified BnaNPR1-like proteins and 6 Arabidopsis homologs were aligned. Identical amino acids are shown in black boxes, and similar amino acids are shown in gray boxes. The BTB/POZ and ankyrin repeat domains are indicated by black bars above the alignment. Several important motifs such as the IκB phosphodegron, LENRV hinge region, NIMIN1/2bindingsite, and NLS1, are also indicated by black bars. The positions of important amino acids in the NLS of AtNPR1 are indicated by black stars above the alignment (PDF 214 kb)

299_2020_2525_MOESM5_ESM.tif

Supplementary File S5 Expression analysis of BnaNPR1-like homologous genes in B. napus infected with S. sclerotiorum. The heat map shows expression changes (log2 fold changes between inoculated and mock-inoculated samples) of BnaNPR1-like genes in leaves of two B. napus lines, J902 and J964, with S. sclerotiorum inoculation at 24, 48, and 96h. The data is from the transcriptomic data (SRA, accession number SRP069360) (TIF 314 kb)

299_2020_2525_MOESM6_ESM.pdf

Supplementary File S6 Disease progression of Sclerotinia sclerotiorum in BnaNPR1-RNAi lines and the untransformed wild-type control. The pictures were taken at leaves from five WT plants and five kanamycin- and PCR-positive plants of line R-19, 20 and 17. WT means the untransformed wild-type control; R-19, 20 and 17 means three independent BnaNPR1-RNA-interfering transgenic lines; hpi means hours post-inoculation (PDF 2120 kb)

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Wang, Z., Ma, LY., Li, X. et al. Genome-wide identification of the NPR1-like gene family in Brassica napus and functional characterization of BnaNPR1 in resistance to Sclerotinia sclerotiorum. Plant Cell Rep 39, 709–722 (2020). https://doi.org/10.1007/s00299-020-02525-z

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