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Genomic taxonomy of the Mediterranei clade of the genus Vibrio (Gammaproteobacteria)

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Abstract

The first genomic study of Mediterranei clade using five type strains (V. mediterranei, V. maritimus, V. variabilis, V. thalassae, and V. barjaei) and fourteen reference strains isolated from marine organisms, seawater, water and sediments of the sea was performed. These bacterial strains were characterised by means of a polyphasic approach comprising 16S rRNA gene, multilocus sequence analysis (MLSA) of 139 single-copy genes, the DNA G + C content, ANI, and in silico phenotypic characterisation. We found that the species of the Mediterranei clade formed two separate clusters based in 16S rRNA gene sequence similarity, MLSA, OrthoANI, and Codon and Amino Acid usage. The Mediterranei clade species showed values between 76 and 95% for ANIb, 84 and 95% for ANIm. The core genome consisted of 2057 gene families and the pan-genome of 13,094 gene families. Based on the genomic analyses performed, the Mediterranei clade can be divided in two clusters, one with the strains of V. maritimus, V. variabilis and two potential new species, and the other cluster with the strains of V. mediterranei, V. thalassae, and V. barjaei.

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Abbreviations

ANI:

Average nucleotide identity

MLSA:

Multilocus sequence analysis

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Acknowledgements

This work was partially funded by CONACYT Project CB-2009-01 132328. We thank P. David Vega-García and M. Carmen Bolán for technical help.

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Authors and Affiliations

  1. Unidad Académica de Ingeniería en Biotecnología, Universidad Politécnica de Sinaloa, Carretera Municipal Libre Km.3 Mazatlán-Higueras, 82199, Mazatlán, Sinaloa, Mexico

    Adrián González-Castillo

  2. CIAD, A.C., Mazatlán Unit for Aquaculture, AP. 711, 82000, Mazatlán, Sinaloa, Mexico

    Julissa Enciso-Ibarra & Bruno Gomez-Gil

Authors
  1. Adrián González-Castillo
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  2. Julissa Enciso-Ibarra
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  3. Bruno Gomez-Gil
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Correspondence to Adrián González-Castillo.

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González-Castillo, A., Enciso-Ibarra, J. & Gomez-Gil, B. Genomic taxonomy of the Mediterranei clade of the genus Vibrio (Gammaproteobacteria). Antonie van Leeuwenhoek 113, 851–859 (2020). https://doi.org/10.1007/s10482-020-01396-4

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