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Persistence of Lymphocystis Disease Virus (LCDV) in Seawater

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Abstract

Lymphocystis disease virus (LCDV), the causative agent of lymphocystis disease (LCD), is a waterborne pathogen that uses the external surfaces, including the gills, as portals to gain access to fish host. However, there are no data on LCDV persistence in the aquatic environment. In this study, the persistence of LCDV in natural (raw), treated (autoclaved and filtered) and synthetic seawater held at 22 and 18 °C has been evaluated. The estimated T99 values for LCDV in seawater ranged from 2.7 to 242 days depending on seawater type and temperature, with the highest value recorded at 22 °C in autoclaved seawater. Microbiota and temperature seem to be the main factors affecting the persistence of LCDV in seawater. The results indicated that LCDV is more stable in treated seawater than most of the fish pathogenic viruses studied so far, supporting the relevance of this medium for the prevalence of LCD in fish farms.

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Acknowledgements

This study was supported by a project from Junta de Andalucía (P12-RNM-2261) granted to J.J. Borrego. R. Leiva-Rebollo was supported by a fellowship from Junta de Andalucía. The authors thank Cristina Zaballa for her collaboration in carrying out the evaluation of the antimicrobial treatment.

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  1. Departamento de Microbiología, Universidad de Málaga, 29071, Málaga, Spain

    Rocío Leiva-Rebollo, Alejandro M. Labella, Estefanía J. Valverde, Dolores Castro & Juan J. Borrego

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  1. Rocío Leiva-Rebollo
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  2. Alejandro M. Labella
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  3. Estefanía J. Valverde
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Correspondence to Juan J. Borrego.

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Leiva-Rebollo, R., Labella, A.M., Valverde, E.J. et al. Persistence of Lymphocystis Disease Virus (LCDV) in Seawater. Food Environ Virol 12, 174–179 (2020). https://doi.org/10.1007/s12560-020-09420-6

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