Abstract
d-Allulose, a C-3 epimer of d-fructose, is a rare sugar and a non-caloric sweetener. d-Allulose is reported to have several health benefits, such as suppressing a rise in postprandial glucose levels and preventing fat accumulation in rodents and humans. Additionally, low HDL-cholesterol levels post-d-allulose feeding were observed in humans but it is unclear how d-allulose decreased HDL-cholesterol levels. It is necessary to research the mechanism of HDL-cholesterol reduction by d-allulose ingestion because low HDL-cholesterol levels are known to associate with increased atherosclerosis risk. We therefore investigated the mechanism by which d-allulose lowers HDL-cholesterol using rat’s primary hepatocytes. Sprague Dawley rats were fed an AIN-93G based diet containing 3% d-allulose for 2 weeks. Thereafter, primary hepatocytes were isolated by perfusion of collagenase. We measured the ability of HDL-cholesterol uptake in hepatocytes and the protein levels of scavenger receptor class B type 1 (SR-B1) as a HDL-cholesterol receptor. d-Allulose enhanced hepatocyte uptake of HDL-cholesterol, with a concurrent increase in hepatic SR-B1 protein levels. The results suggest that d-allulose enhances HDL-cholesterol uptake into the liver by increasing SR-B1 expression. It is estimated that HDL-cholesterol levels decreased accordingly. Since SR-B1 overexpression would decrease HDL-cholesterol levels, reportedly preventing atherosclerosis development, d-allulose could be a useful sweetener for atherosclerosis prevention.
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We would like to thank Editage (www.editage.com) for English language editing.
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AK wrote the manuscript. AK and TI participated in the experimental work and collected and analyzed data. KM, BS and MS supervised the study and commented on the manuscript. All authors have read and approved the final version of the manuscript.
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The study was funded by Matsutani Chemical Industry Co. Ltd. (Itami, Japan). A.K. and T.I. are employees for this company.
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Kanasaki, A., Iida, T., Murao, K. et al. d-Allulose enhances uptake of HDL-cholesterol into rat’s primary hepatocyte via SR-B1. Cytotechnology 72, 295–301 (2020). https://doi.org/10.1007/s10616-020-00378-8
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DOI: https://doi.org/10.1007/s10616-020-00378-8