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Hyphopichia lachancei, f.a., sp. nov., a yeast species from diverse origins

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Abstract

Three strains originating from insect frass in South Africa, yellow foxglove in Hungary and soil in France, were characterised phenotypically and by sequencing of the D1/D2 domain of the large subunit and the ITS1-5.8S-ITS2 (ITS)-region of the rRNA gene. The strains have identical D1/D2 domain sequences and only one strain shows a 1 bp indel in a 9 bp homopolymer A/T repeat within the ITS-region. Based on sequence analysis Hyphopichia burtonii is the closest related species. The investigated strains differ from the type strain of H. burtonii by 1.9% (9 substitutions and an indel) in the D1/D2 domain and by 23 substitutions and 21–22 indels in the ITS-region. Since the sequence variability is very low among the three strains and the sequence divergence with the closely related H. burtonii exceeds the level generally encountered between species we propose the new species Hyphopichia lachancei f.a., sp. nov. to accommodate the three novel strains. From H. burtonii the new species can be distinguished phenotypically by its inability to ferment cellobiose and by the formation of endospores (Holotype: CBS 5999T; Isotype: NCAIM Y.02228T; MycoBank no.: MB833616).

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Acknowledgements

This work was supported by The Deutsche Bundesstiftung Umwelt DBU (German Federal Environmental Foundation) [34053/01-32 to M.B-H] and by the State Secretariat for Education of the Hungarian Ministry of Human Capacities and by the European Union and co-financed by the European Social Fund [grant agreement no. EFOP-3.6.3-VEKOP-16-2017-00005 to G.P. and D.D.].

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Authors and Affiliations

  1. Laboratory for Wine Microbiology, Department International Business, Heilbronn University, Max-Planck-Str. 39, 74081, Heilbronn, Germany

    Michael Brysch-Herzberg & Martin Seidel

  2. Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands

    Marizeth Groenewald

  3. National Collection of Agricultural and Industrial Microorganisms, Faculty of Food Science, Szent István University, Somlói út 14-16, Budapest, 1118, Hungary

    Dénes Dlauchy & Gábor Péter

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  1. Michael Brysch-Herzberg
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  2. Marizeth Groenewald
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  3. Dénes Dlauchy
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  4. Martin Seidel
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  5. Gábor Péter
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Contributions

MBH wrote parts of the paper, produced the figures, analysed data and performed the light microscopic analysis. MG wrote parts of the paper, made physiological tests and supported the data analysis. DD and MS performed physiological tests and did molecular genetic analysis. GP wrote parts of the paper.

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Correspondence to Michael Brysch-Herzberg.

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Brysch-Herzberg, M., Groenewald, M., Dlauchy, D. et al. Hyphopichia lachancei, f.a., sp. nov., a yeast species from diverse origins. Antonie van Leeuwenhoek 113, 773–778 (2020). https://doi.org/10.1007/s10482-020-01387-5

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