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Inherited chromosomally integrated HHV-6 possibly modulates human gene expression

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Abstract

Approximately, 1% of human population possesses a copy of human herpesvirus 6A and 6B (HHV-6A/B) in the genome. This viral element is referred to as inherited chromosomally integrated HHV-6A/B (iciHHV-6A/B) and is encoded in all of their cells. A recent study revealed that iciHHV-6A/B potentially increases the immune responses against HHV-6. However, it remains unclear whether iciHHV-6A/B affects human gene expression. Here, we perform global transcriptome analysis using the datasets obtained from various human tissues. We detected two and four individuals positive for iciHHV-6A and iciHHV-6B, respectively, and revealed that the transcriptional expression of iciHHV-6A/B was sporadic in the human body. Transcriptome analysis identified the human genes differentially expressed between iciHHV-6A/B-positive and -negative individuals. Particularly, the expression of some genes encoding immunoglobulins decreased in sigmoid colon of iciHHV-6A/B-positive individuals. Our findings suggest that iciHHV-6A/B may be associated with human health maintenance.

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Acknowledgments

We would like to thank Mai Suganami (Division of Systems Virology, Institute of Medical Science, the University of Tokyo, Japan) for technical support. The super-computing resource, SHIROKANE, was provided by Human Genome Center, The Institute of Medical Science, the University of Tokyo, Japan. The Genotype-Tissue Expression (GTEx) Project was supported by the Common Fund of the Office of the Director of the National Institutes of Health, and by NCI, NHGRI, NHLBI, NIDA, NIMH, and NINDS.

Funding

This study was supported in part by JST CREST (to KS) including AIP challenge program (to RK); AMED J-PRIDE JP19fm0208006 (to KS); AMED Research Program on HIV/AIDS JP19fk0410014 (to KS) and JP19fk0410019 (to KS); JSPS Scientific Research B JP18H02662 (to KS), Scientific Research on Innovative Areas JP16H06429 (to KS), JP16K21723 (to KS), JP17H05813 (to KS), and JP19H04826 (to KS), Fund for the Promotion of Joint International Research (Fostering Joint International Research) JP18KK0447 (to KS); JSPS Research Fellow PD JP19J01713 (to JI); Ichiro Kanehara Foundation (to KS); Lotte Foundation (to KS); Mochida Memorial Foundation for Medical and Pharmaceutical Research (to KS); Sumitomo Foundation (to KS).

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Authors and Affiliations

  1. Division of Systems Virology, Department of Infectious Disease Control, International Research Center for Infectious Diseases, Institute of Medical Science, The University of Tokyo, Tokyo, 1088639, Japan

    Ryuichi Kumata, Jumpei Ito & Kei Sato

  2. CREST, Japan Science and Technology Agency, Saitama, 3220012, Japan

    Kei Sato

Authors
  1. Ryuichi Kumata
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  2. Jumpei Ito
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  3. Kei Sato
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Contributions

All authors conceived and designed the study, and wrote the paper. RK and JI performed bioinformatic analysis.

Corresponding author

Correspondence to Kei Sato.

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The authors declare that they have no conflict of interest.

Ethical approval

The utilization of the GTEx dataset was authorized through the Database of Genotypes and Phenotypes (dbGaP; https://dbgap.ncbi.nlm.nih.gov) for the following project: “Screening of subclinical viral infections in healthy human tissues (#19481)”.

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Kumata, R., Ito, J. & Sato, K. Inherited chromosomally integrated HHV-6 possibly modulates human gene expression. Virus Genes 56, 386–389 (2020). https://doi.org/10.1007/s11262-020-01745-5

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  • DOI: https://doi.org/10.1007/s11262-020-01745-5

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