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Sugar transporter genes in grass carp (Ctenopharyngodon idellus): molecular cloning, characterization, and expression in response to different stocking densities

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Abstract

Glucose and fructose play a central role in the metabolism and cellular homeostasis of organisms. Their absorption is co-mediated by two families of glucose transporters, Na+-coupled glucose co-transporters (SGLTs) and facilitative Na+-independent sugar carriers (GLUTs), in the intestine. However, limited information has been available on these transporters in fish. Therefore, we studied glut2, sglt1, and sglt4 genes in grass carp (Ctenopharyngodon idellus). The full-length cDNAs of glut2 was 2308 bp, with an open reading frame (ORF) of 503 amino acids (AAs). The full-length cDNAs of sglt1 was 2890 bp, with an ORF of 658 AAs. Additionally, the full-length cDNAs of sglt4 was 2090 bp, with an ORF encoding 659 AAs. The three deduced AA sequences showed high homology between grass carp and other cyprinid fish species. Based on homology modeling, three-dimensional models of GLUT2, SGLT1, and SGLT4 proteins were created and transmembrane domains were noted. glut2, sglt1, and sglt4 were abundantly expressed in the anterior and mid intestine. In particular, glut2 was markedly expressed in liver (P < 0.05). Additionally, the results indicated that different stocking densities (0.9 or 5.9 kg m−2) did not alter intestinal section-dependent expression patterns of the three transporter genes. However, high stocking density impacted segmental mRNA expression levels. This work demonstrated that mRNA expression of sugar transporter genes in the fish intestine was segment specific, and crowding stress may affect the activity of intestinal sugar transporters. These results provided new insights into the relationship between crowding stress and intestinal sugar transporters in fish.

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Funding

This study was supported by the Earmarked Fund for China Agriculture Research System (Project no. CARS-45), the National Natural Foundation of China (Project no. 31502140) and the Fundamental Research Funds for the Central Universities (Project no. 2662015PY119).

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Authors and Affiliations

  1. College of Fisheries, Hubei Provincial Engineering Laboratory for Pond Aquaculture, Huazhong Agricultural University, Shizishan Street, Hongshan District, Wuhan, 430070, China

    Xiao Liang, Fengying Yan, Mei Xiong, Haishan Wang, Kommaly Onxayvieng, Rong Tang, Li Li, Xi Zhang, Wei Chi & Dapeng Li

  2. College of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, China

    Yu Gao

  3. Department of livestock and fisheries, Ministry of agriculture and forestry, 6644, Vientiane, Laos

    Kommaly Onxayvieng

  4. University of Zagreb, Faculty of Agriculture, Svetošimunska cesta 25, 10000, Zagreb, Croatia

    Marina Piria, Mirna Mrkonjić Fuka & Ana Gavrilović

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  1. Xiao Liang
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  2. Fengying Yan
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  3. Yu Gao
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  4. Mei Xiong
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  8. Li Li
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  9. Xi Zhang
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  10. Wei Chi
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  11. Marina Piria
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  12. Mirna Mrkonjić Fuka
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  13. Ana Gavrilović
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  14. Dapeng Li
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Contributions

X.L. performed the experiment, analyzed data, and wrote the manuscript; F.Y., M.X., H.W., and K.O. cultured the fish and analyzed the growth data; G.Y., R.T., L.L., X.Z., W.C., M.P., M.F., and A.G. revised the manuscript. All authors reviewed the manuscript.

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Correspondence to Dapeng Li.

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Liang, X., Yan, F., Gao, Y. et al. Sugar transporter genes in grass carp (Ctenopharyngodon idellus): molecular cloning, characterization, and expression in response to different stocking densities. Fish Physiol Biochem 46, 1039–1052 (2020). https://doi.org/10.1007/s10695-020-00770-3

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