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MicroRNA-498 inhibits the proliferation, migration and invasion of gastric cancer through targeting BMI-1 and suppressing AKT pathway

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Abstract

Recently, microRNA-498 (miR-498) plays important effect in human cancers. Nonetheless, the role of miR-498 is still unclear in gastric cancer (GC). Therefore, this study was designed to investigate the function of miR-498 in GC tissues and cell lines (SGC-7901, BGC-823, MGC-803). The expressions of miR-498 and BMI-1 were examined in GC tissues via the RT-qPCR assay. The function of miR-498 was investigated through MTT and transwell assays. The relationship between miR-498 and BMI-1 was testified by dual luciferase assay. The protein expression of EMT markers, AKT pathway markers and BMI-1 was measured through western blot. The expression of miR-498 was decreased in GC tissues which predicted poor prognosis of GC patients. Moreover, functional analyses show that the overexpression of miR-498 inhibited the progression of GC. Furthermore, BMI-1 was a direct target of miR-498 which was upregulated in GC. Especially, the upregulation of BMI-1 recovered the suppressive effect of miR-498 in GC. In addition, miR-498 inhibited the metastasis and proliferation of GC cells through blocking EMT and AKT pathway. MiR-498, by targeting BMI-1, presents a plethora of tumor suppressor activities in GC cells.

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Data availability

The datasets used and/or analyzed during the present study are available from the corresponding author on reasonable request.

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Authors and Affiliations

  1. Department of Radiotherapy, Yantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai, 26400, Shandong Province, China

    Dong You, Dawei Wang & Peiji Liu

  2. Qingdao University Medical College, Qingdao, Shandong Province, China

    Yuning Chu & Xueying Zhang

  3. Department of Gastroenterology, The Affiliated Hospital of Qingdao University, Qingdao, 266003, Shandong Province, China

    Xueli Ding, Xiaoyu Li, Tao Mao, Xue Jing & Zibin Tian

  4. Department of Radiology, Yantai Yuhuangding Hospital Affiliated to Qingdao University, East of Yuhuangding Road, Yantai, 26400, Shandong Province, China

    Yinghua Pan

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  1. Dong You
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Correspondence to Yinghua Pan.

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This study was approved by the Institutional Ethics Committee of The Affiliated Hospital of Qingdao University (Qingdao, China) and was performed according to the guidelines of the Declaration of Helsinki. Informed consent was obtained from all patients.

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You, D., Wang, D., Liu, P. et al. MicroRNA-498 inhibits the proliferation, migration and invasion of gastric cancer through targeting BMI-1 and suppressing AKT pathway. Human Cell 33, 366–376 (2020). https://doi.org/10.1007/s13577-019-00313-w

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