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Molecular Characteristics, Phylogeny and Expression Profile of the PTEN Gene in Goats

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Abstract

Phosphatase and the tensin homologue deleted on chromosome ten (PTEN) has pleiotropic effects on cell growth, organ development, glucose metabolism and insulin resistance in mammals. In the present study, we investigated the molecular characteristics, phylogeny and expression profile of the PTEN gene in different tissues of Jianzhou Daer goats. In this study, eight different tissues from E90, E135 and D90 female goats were collected to quantify the expression pattern of the PTEN gene using quantitative real-time PCR (qPCR), western blotting and FISH. In addition, the dynamic expression of PTEN was also determined during the differentiation of goat precursor adipose cells. A 1212-bp fragment (accession number MG923848), encoding a 403-amino acid protein with a putative molecular weight of 47.14 kDa, was identified in Jianzhou Daer goats by reverse-transcription polymerase chain reaction (RT-PCR). The phylogenetic tree showed that caprine PTEN had a relatively close relationship with ovine PTEN and bovine PTEN. qPCR revealed that PTEN was highly expressed in the liver, lung and spleen, while the lowest expression levels were observed in muscle tissues (P < 0.05). Moreover, the expression of the PTEN gene showed a decreasing trend during the differentiation of goat precursor adipose cells. RNA in situ hybridization yielded a consistent result with the qPCR data. Indeed, low protein expression was found in psoas major muscle and longissimus dorsi muscle, as well as in kidney and liver. However, PTEN protein was expressed at the highest level in the brain. The expression levels of PTEN mRNA and protein were inconsistent with each other, possibly because of post-transcriptional regulation. The findings obtained in our study lay a foundation for further investigations examining the caprine PTEN gene in embryo and organ development.

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Acknowledgements

This work was supported by the National Key Research and Development Program of China (2018YFD0502002) and the Chinese Domestic Animal Germplasm Resources Infrastructure.

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  1. Tao Zhong and Jixuan Zhou have contributed equally.

Authors and Affiliations

  1. Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China

    Tao Zhong, Jingxuan Zhou, Siyuan Zhan, Linjie Wang, Lili Niu, Jiazhong Guo, Li Li & Hongping Zhang

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  1. Tao Zhong
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Zhong, T., Zhou, J., Zhan, S. et al. Molecular Characteristics, Phylogeny and Expression Profile of the PTEN Gene in Goats. Biochem Genet 58, 399–411 (2020). https://doi.org/10.1007/s10528-020-09947-0

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