Abstract
Cadmium (Cd), a substance with one of the most critical health hazard indices, can cause damage to both the blood and kidneys and accumulates in the body at last. The present work studied the toxicological effects of Cd and the therapeutic effects of metallothionein (MT) and vitamin E (VE) on the trunk kidney and blood of freshwater grass carp (Ctenopharyngodon idellus). Grass carp were divided into three groups: Cd + phosphate-buffered saline (PBS) group, Cd + VE group, and the Cd + MT group. Fish were injected with CdCl2 on the first day and then VE, MT, or PBS was administered 4 days post-injection. Fish not injected with Cd were used as a negative control. The blood and trunk kidney amassed Cd and suffered severe damage in the forms of organ toxicity cytotoxicity, and immunotoxicity. However, the MT reduced the Cd content in the trunk kidney and blood and partially stabilized the damaged organs. Treatment with VE, however, only demonstrated weaker protection against on Cd-induced toxicity. The results indicate that exogenous MT may play an essential role in restoring homeostasis of the Cd-poisoned urinary and circulatory system and that it may help eliminate Cd in aquatic animals.
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References
Al-Asgah NA, Abdel-Warith AWA, Younis ESM, Allam HY (2015) Haematological and biochemical parameters and tissue accumulations of cadmium in Oreochromis niloticus exposed to various concentrations of cadmium chloride. Saudi J Biol Sci 22(5):543–550
Albergoni V, Viola A (1995) Effects of cadmium on lymphocyte proliferation and macrophage activation in catfish, Ictalurus melas. Fish Shellfish Immunol 5(4):301–311
Blaxhall PC, Daisley KW (2010) Routine haematological methods for use with fish blood. J Fish Biol 5(6):771–781
Chen CY, Zhou CJ, De-Yong PU (2009) Apoptosis induction of blood cells in Myxocyprinus asiaticus by cadmium stress. J Hydroecol 2(4):98–102
Choi CY, An KW, Nelson ER, Habibi HR (2007) Cadmium affects the expression of metallothionein (MT) and glutathione peroxidase (GPX) mRNA in goldfish, Carassius auratus. Comp Biochem Phys C 145(4):595–600
Conti ME (1997) The content of heavy metals in food packaging paper: an atomic absorption spectroscopy investigation. Food Control 8(3):131–136
Duan Y, Duan J, Feng Y, Huang X, Fan W, Wang K, Ouyang P, Deng Y, Du Z, Chen D (2018) Hepatoprotective activity of vitamin E and metallothionein in cadmium-induced liver injury in Ctenopharyngodon idellus. Oxidative Med Cell Longev 2018(4):1–12
Eum KD, Lee MS, Paek D (2008) Cadmium in blood and hypertension. Sci Total Environ 407(1):147–153
Ezedom T (2018) Cadmium and arsenic induced toxicity through a controlled food chain on plasma lipid profile and endogenous metabolites of rats. Ann Biomed Sci 17(1):1–7
Ghiasi F, Mirzargar SS, Badakhshan H, Shamsi S (2010) Effects of low concentration of cadmium on the level of lysozyme in serum, leukocyte count and phagocytic index in Cyprinus carpio under the wintering conditions. J Fis Aquat Sci 5(2):113–119
Huttenhuis H, Taverne-Thiele A, Grou C, Bergsma J, Saeij JC, Rombout J (2006) Ontogeny of the common carp (Cyprinus carpio L.) innate immune system. Dev Comp Immunol 30(6):557–574
Järup L, Åkesson A (2009) Current status of cadmium as an environmental health problem. Toxicol Appl Pharmacol 238(3):201–208
Jing W, Lang L, Lin Z, Liu N, Wang L (2019) Cadmium bioaccumulation and elimination in tissues of the freshwater mussel Anodonta woodiana. Chemosphere 219:321–327
Kim MJ, Hong JH (2003) Effect of vitamin E on cadmium accumulation and excretion in chronic cadmium poisoned rats. Korean Nutr Soc 36(7):691–698
Kukner A, Colakoglu N, Kara H, Oner H, Ozogul C, Ozan E (2007) Ultrastructural changes in the kidney of rats with acute exposure to cadmium and effects of exogenous metallothionein. Biol Trace Elem Res 119(2):137–146
Lee AS (2007) GRP78 induction in cancer: therapeutic and prognostic implications. Cancer Res 67(8):3496–3499
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods 25(4):402–408
Mao WP, Ye JL, Guan ZB, Zhao JM, Zhang C, Zhang NN, Jiang P, Tian T (2007) Cadmium induces apoptosis in human embryonic kidney (HEK) 293 cells by caspase-dependent and -independent pathways acting on mitochondria. Toxicol in Vitro 21(3):343–354
Masahiko S, Toshiyuki K, Chiharu T (2003) Low dose exposure to cadmium and its health effects. (3) Toxicity in laboratory animals and cultured cells. J Nippon Med Sch 57(4):615–623
Min KS, Onosaka S, Tanaka K (1996) Renal accumulation of cadmium and nephropathy following long-term administration of cadmium-metallothionein. Toxicol Appl Pharmacol 141(1):102–109
Na S, Zhan-Gang X, Qiong-Lin G (2010) The distribution of cd4l,cd8α and cd8β expression cells in immuno-organs of carp. Acta Hydrobiol Sin 34(3):673–678
Nikolic R, Krstic N, Jovanovic J, Kocic G, Cvetkovic TP, Radosavljevic-Stevanovic N (2015) Monitoring the toxic effects of Pb, cd and cu on hematological parameters of Wistar rats and potential protective role of lipoic acid and glutathione. Toxicol Ind Health 31(3):239–246
Ognjanovic BI, Pavlovic SZ, Maletic SD, Zikic RV, Stajn AS, Radojicic RM, Saicic ZS, Petrovic VM (2003) Protective influence of vitamin E on antioxidant defense system in the blood of rats treated with cadmium. Physiol Res 52(5):563–570
Organization, W. H (2010) Exposure to cadmium: a major public health concern. Preventing Disease Through Healthy Environments:3–6
Pathak N, Khandelwal S (2008) Impact of cadmium in T lymphocyte subsets and cytokine expression: differential regulation by oxidative stress and apoptosis. Biometals 21(2):179–187
Patra RC, Rautray AK, Swarup D (2011) Oxidative stress in lead and cadmium toxicity and its amelioration. Vet Med Int 2011:457327–457327
Soisungwan S, Garrett SH, Mary Ann S, Sens DA (2010) Cadmium, environmental exposure, and health outcomes. Environ Health Perspect 118(2):182–190
Tarasub N, Junseecha T, Tarasub C, Ayutthaya WDN (2012) Protective effects of curcumin, vitamin C, or their combination on cadmium-induced hepatotoxicity. J Basic Clin Pharm 3(2):273–281
Thijssen S, Lambrichts I, Maringwa J, Van KE (2007) Changes in expression of fibrotic markers and histopathological alterations in kidneys of mice chronically exposed to low and high cd doses. Toxicology 238(2):200–210
Wang J-N, Zhao X-M, Hu G-C, Zhong S-X, Yao L-A, Ma Q-L, Xu Z-C (2019) Distribution and bioaccumulation characteristics of cadmium in fish species from the Longjiang River in the Guangxi autonomous region. Environ Sci 40(1):488–495
Wang Q, Wei S (2018) Cadmium affects blood pressure and negatively interacts with obesity: findings from NHANES 1999-2014. Sci Total Environ 643:270–276
Xu LR, Carr MM, Bland AP, Hall GA (1993) Histochemistry and morphology of porcine mast cells. Histochem J 25(7):516–522
Yabe J, Nakayama SM, Ikenaka Y, Yohannes YB, Bortey-Sam N, Kabalo AN, Ntapisha J, Mizukawa H, Umemura T, Ishizuka M (2018) Lead and cadmium excretion in feces and urine of children from polluted townships near a lead-zinc mine in Kabwe, Zambia. Chemosphere 202:48–55
Yogendranathan N, Herath HMMTB, Sivasundaram T, Constantine R, Kulatunga A (2017) A case report of zinc phosphide poisoning: complicated by acute renal failure and tubulo interstitial nephritis. Bmc Pharmacol Toxicol 18(37):1–6
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We thank all authors for stimulating discussions and support.
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This research was supported by grants from the double support plan of Sichuan Agricultural University (03571775).
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All protocols were approved by the animal care and use committee of Sichuan agricultural university, following the guidelines on animal experiments which under permit No.DY-S20144657.
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Huang, X., Xiong, G., Feng, Y. et al. Protective effects of metallothionein and vitamin E in the trunk kidney and blood of cadmium poisoned Ctenopharyngodon idellus. Fish Physiol Biochem 46, 1053–1061 (2020). https://doi.org/10.1007/s10695-020-00771-2
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DOI: https://doi.org/10.1007/s10695-020-00771-2