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Fatty acids profiles and estimation of the biodiesel quality parameters from Rhodotorula spp. from Antarctica

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Abstract

Objective

Oleaginous yeasts are a renewable and alternative source of oil for third-generation biodiesel. This work aimed to evaluate the effects of glucose concentration (30–100 g L−1) on growth, lipid synthesis, and fatty acids (FA) profile of three Rhodotorula spp. (R. glacialis R15, R. glutinis R4, and R. glutinis R48) isolated from Antarctica, and estimate the key quality parameters of the biodiesel produced by yeasts to confirm their potential as feedstocks for third-generation biodiesel synthesis.

Results

Yeasts accumulated 50–69.5% of lipids (w/w) under nitrogen-limitation and glucose-excess (C/N = 40–133). Glucose concentration increase influenced positively lipid accumulation (69.5% w/w) and FA profile of R. glacialis R15. Lipid accumulation (53% on average) of R. glutinis strains was not significantly affected by glucose concentration; content of saturated (~ 30%) and polyunsaturated FA (~ 29–30%) was slightly influenced. FA profiles of lipids synthesized by R15, R4, and R48 are similar to vegetable oils used in biodiesel industry with C16 and C18 FA (95–99%) as the major components, and contain mainly oleic (C18:1), palmitic (C16:0), and linoleic (C18:2) acids, which are suitable for biodiesel synthesis. Estimated fuel properties for biodiesel produced by R15, R4, and R48 satisfied all the criteria established by ASTM D6751 and EN 14214 with good cetane number, iodine value, and oxidation stability. An improvement in biodiesel quality of R15 was observed with the glucose increase. The best global properties of biodiesel from R4 were obtained with 30 g L−1 of glucose.

Conclusions

Rhodotorula spp. from Antarctica are promising candidates for third-generation biodiesel synthesis.

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Acknowledgements

The authors would like to thank specially to Dirección Nacional del Antártico (DNA) and Instituto Antártico Argentino (IAA) for supporting the Antarctic expeditions where the samples were collected under an agreement with Dr. Walter Mac Cormack and the Environmental Microbiology Group.

Funding

This work was supported by the Agencia Nacional de Promoción Científica y Tecnológica FONCYT (PICT 1154-2013; PICT3083-2016; PICT2013-2016); the Consejo Nacional de Investigaciones Científicas y Técnicas CONICET (PUE-2016-0012, PIP0677-2015) and the Secretaría de Ciencia, Arte e Innovación Tecnológica de la Universidad Nacional de Tucumán SCAIT UNT (PIUNT D509).

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Authors and Affiliations

  1. PROIMI-CONICET (Planta Piloto de Procesos Industriales Microbiológicos), Av. Belgrano y Caseros, T4001MVB, Tucumán, Argentina

    Silvana Carolina Viñarta, María Virginia Angelicola & Lucía Inés Castellanos de Figueroa

  2. INSIBIO, CONICET-UNT (Instituto Superior de Investigaciones Biológicas), Chacabuco 461, T4000ILI, Tucumán, Argentina

    Silvana Carolina Viñarta & Manuel Javier Aybar

  3. Biología de los Microorganismos, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Catamarca, 4700, Catamarca, Argentina

    Silvana Carolina Viñarta

  4. CERELA-CONICET (Centro de Referencia Para Lactobacilos), Chacabuco 145, T4000ILC, Tucumán, Argentina

    Carina Van Nieuwenhove

  5. Microbiología Superior, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, 4000, Tucumán, Argentina

    Lucía Inés Castellanos de Figueroa

  6. Laboratory of Fungal Biotechnology, PROIMI-CONICET (Planta Piloto de Procesos Industriales Microbiológicos), Av. Belgrano y Caseros, T4001MVB, Tucumán, Argentina

    Silvana Carolina Viñarta

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  1. Silvana Carolina Viñarta
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  2. María Virginia Angelicola
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Correspondence to Silvana Carolina Viñarta.

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Viñarta, S.C., Angelicola, M.V., Van Nieuwenhove, C. et al. Fatty acids profiles and estimation of the biodiesel quality parameters from Rhodotorula spp. from Antarctica. Biotechnol Lett 42, 757–772 (2020). https://doi.org/10.1007/s10529-020-02796-2

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