Skip to main content

Advertisement

SpringerLink
Log in

Production and characterization of bacterial cellulose obtained by Gluconacetobacter xylinus utilizing the by-products from Baijiu production

  • Research Paper
  • Published:
Bioprocess and Biosystems Engineering Aims and scope Submit manuscript

Abstract

Bacterial cellulose (BC) has extensive application prospects in many fields in view of its unique characteristics. However, the large-scale applications of BC are severely limited because of relatively low BC productivity and high cost of culture medium. Herein, the distiller’s grain enzymatic hydrolysate (DEH) and yellow water were successfully combined as an effective substitute (the best distiller’s grains–yellow water medium, BDY medium) for traditional Hestrin–Schramm medium (HS medium) for BC production by Gluconacetobacter xylinus through the response surface methodology. The BC production in BDY medium was significantly enhanced to 7.42 g/l with BC conversion yield of 42.4% after 7 days static cultivation, which was 3.72-fold and 3.37-fold higher than that in HS medium, respectively. The structure and properties of BC membranes produced in HS and BDY medium were evaluated by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), field emission scanning electron microscopy (FE-SEM), thermogravimetric analysis (TGA) and hydrophilicity analysis. There was no significant difference between BC samples produced in the HS and BDY medium, indicating that BDY, as abundant and inexpensive substrates, can effectively replace HS medium to enhance BC production. The employment of distiller’s grains and yellow water to BC production not only is conducive to achieve industrial production of BC, but also can effectively realize the recycling of waste from Baijiu distillery.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Huang Y, Zhu C, Yang J, Nie Y, Chen C, Sun D (2014) Recent advances in bacterial cellulose. Cellulose 21:1–30

    Google Scholar 

  2. Singhsa P, Narain R, Manuspiya H (2018) Physical structure variations of bacterial cellulose produced by different Komagataeibacter xylinus strains and carbon sources in static and agitated conditions. Cellulose 25:1–11

    Google Scholar 

  3. Li Z, Wang LF, Hua JC, Jia SR, Zhan JF, Liu H (2015) Production of nano bacterial cellulose from waste water of candied jujube-processing industry using Acetobacter xylinum. Carbohydr Polym 120:115–119

    CAS  PubMed  Google Scholar 

  4. Pacheco G, Nogueira CR, Meneguin AB, Silva MCC, Trovatti E, Machado RTA et al (2017) Development and characterization of bacterial cellulose produced by cashew tree residues as alternative carbon source. Ind Crops Prod 107:13–19

    CAS  Google Scholar 

  5. Ashori A, Sheykhnazari S, Tabarsa T, Shakeri A, Golalipour M (2012) Bacterial cellulose/silica nanocomposites: preparation and characterization. Carbohydr Polym 90:413–418

    CAS  PubMed  Google Scholar 

  6. Dayal MS, Goswami N, Sahai A, Jain V, Mathur G, Mathur A (2013) Effect of media components on cell growth and bacterial cellulose production from Acetobacteraceti MTCC 2623. Carbohydr Polym 94:12–16

    CAS  PubMed  Google Scholar 

  7. Di Z, Shi Z, Ullah MW, Li S, Yang G (2017) A transparent wound dressing based on bacterial cellulose whisker and poly (2-hydroxyethyl methacrylate). Int J Biol Macromol 105:638–644

    CAS  PubMed  Google Scholar 

  8. Fu L, Zhang J, Yang G (2013) Present status and applications of bacterial cellulose-based materials for skin tissue repair. Carbohydr Polym 92:1432–1442

    CAS  PubMed  Google Scholar 

  9. Helenius G, Bäckdahl H, Bodin A, Nannmark U, Gatenholm P, Risberg B (2010) In vivo biocompatibility of bacterial cellulose. J Biomed Mater Res Part A 76A:431–438

    Google Scholar 

  10. Esa F, Tasirin SM, Rahman NA (2014) Overview of bacterial cellulose production and application. Agric Agric Sci Procedia 2:113–119

    Google Scholar 

  11. Velasquez-Riano M, Bojaca V (2017) Production of bacterial cellulose from alternative low-cost substrates. Cellulose 24:2677–2698

    CAS  Google Scholar 

  12. Cheng Z, Yang R, Liu X, Liu X, Chen H (2017) Green synthesis of bacterial cellulose via acetic acid pre-hydrolysis liquor of agricultural corn stalk used as carbon source. Biores Technol 234:8–14

    CAS  Google Scholar 

  13. Kurosumi A, Sasaki C, Yamashita Y, Nakamura Y (2009) Utilization of various fruit juices as carbon source for production of bacterial cellulose by Acetobacter xylinum NBRC 13693. Carbohydr Polym 76:333–335

    CAS  Google Scholar 

  14. Wu JM, Liu RH (2012) Thin stillage supplementation greatly enhances bacterial cellulose production by Gluconacetobacter xylinus. Carbohydr Polym 90(1):116–121

    CAS  PubMed  Google Scholar 

  15. Hong F, Zhu YX, Yang G et al (2011) Wheat straw acid hydrolysate as a potential cost-effective feedstock for production of bacterial cellulose. J Chem Technol Biotechnol 86(5):675–680

    CAS  Google Scholar 

  16. Vazquez A, Foresti ML, Cerrutti P, Galvagno M (2013) Bacterial Cellulose from simple and low cost production media by Gluconacetobacter xylinus. J Polym Env 21:545–554

    CAS  Google Scholar 

  17. Zuo SC, Yang HQ, Zou W (2016) Research progress on the utilization of distiller's grains as resources. Food Ind 37:246–249

    CAS  Google Scholar 

  18. Wang ZG, Wei YL, Wei MS, Wang XL, Xiang D (2008) Effect of organic acids on bacterial cellulose production in defined medium by Acetobater xylinum. Food Sci 29:295–297

    CAS  Google Scholar 

  19. Zhang LP, Lu HM, Dai R, Dai LX, Jiang XL (2014) Study on the function of ethanol and organic acids to Acetobacter xylinum synthetic bacterial cellulose. Food Ind Sci Technol 35:161–165

    Google Scholar 

  20. Hestrin S, Schramm M (1954) Synthesis of cellulose by Acetobacter xylinum. II. Preparation of freeze-dried cells capable of polymerizing glucose to cellulose. Biochem J 58:345–352

    CAS  PubMed  PubMed Central  Google Scholar 

  21. Erbas Kiziltas E, Kiziltas A, Gardner DJ (2015) Synthesis of bacterial cellulose using hot water extracted wood sugars. Carbohydr Polym 124:131–138

    CAS  PubMed  Google Scholar 

  22. Lever M (1977) Carbohydrate determination with 4-hydroxybenzoic acid hydrazide (PAHBAH): effect of bismuth on the reaction. Anal Biochem 81:21–27

    CAS  PubMed  Google Scholar 

  23. Du R, Zhao F, Peng Q, Zhou ZJ, Han Y (2018) Production and characterization of bacterial cellulose produced by Gluconacetobacter xylinus isolated from chinese persimmon vinegar. Carbohydr Polym 194:20–207

    Google Scholar 

  24. Żywicka A, Junka AF, Szymczyk P, Chodaczek G, Grzesiak J, Sedghizadeh PP et al (2018) Bacterial cellulose yield increased over 500% by supplementation of medium with vegetable oil. Carbohydr Polym 199:294–303

    PubMed  Google Scholar 

  25. Huang C, Yang XY, Xiong L, Guo HJ, Luo J et al (2015) Evaluating the possibility of using acetone-butanol-ethanol (ABE) fermentation wastewater for bacterial cellulose production by Gluconacetobacter xylinus. Lett Appl Microbiol 60:491–496

    CAS  PubMed  Google Scholar 

  26. Qi GX, Luo MT, Huang C et al (2017) Comparison of bacterial cellulose production by\r, Gluconacetobacter xylinus on bagasse acid and enzymatic hydrolysates. J Appl Polym Sci 134:45066

    Google Scholar 

  27. Yang Y, Jia J, Xing J, Chen J, Lu S (2013) Isolation and characteristics analysis of a novel high bacterial cellulose producing strain Gluconacetobacter intermedius Cis26. Carbohydr Polym 92:2012–2017

    CAS  PubMed  Google Scholar 

  28. Masaoka S, Ohe T, Sakota N (1993) Production of cellulose from glucose by Acetobacter xylinum. J Ferment Bioeng 75:18–22

    CAS  Google Scholar 

  29. Embuscado ME, Marks JS, Bemiller JN (1994) Bacterial cellulose. I. Factors affecting the production of cellulose by Acetobacter xylinum. Food Hydrocolloids 8:407–418

    CAS  Google Scholar 

  30. Jung HI, Jeong JH, Lee OM, Park GT, Kim KK, Park HC et al (2010) Influence of glycerol on production and structural–physical properties of cellulose from Acetobacter sp. V6 cultured in shake flasks. Biores Technol 101:3602–3608

    CAS  Google Scholar 

  31. Kuo CH, Chen JH, Liou BK, Lee CK (2015) Utilization of acetate buffer to improve bacterial cellulose production by Gluconacetobacter xylinus. Food Hydrocolloids 53:98–103

    Google Scholar 

  32. Zhang LP, Lu HM, Dai R, Dai LX, Jiang XL (2014) Study on the function of ethanol and organic acid to Acetobacter xylinus synthetic bacterial cellulose. Sci Technol Food Ind 35:161–165

    Google Scholar 

  33. Stumpf TR, Pértile RAN, Rambo CR, Porto LM (2013) Enriched glucose and dextrin mannitol-based media modulates fibroblast behavior on bacterial cellulose membranes. Mater Sci Eng C 33:4739–4745

    CAS  Google Scholar 

  34. Ul-Islam M, Ha JH, Khan T, Park JK (2013) Effects of glucuronic acid oligomers on the production, structure and properties of bacterial cellulose. Carbohydr Polym 92:360–366

    CAS  PubMed  Google Scholar 

  35. Castro C, Zuluaga R, Alvarez C, Putaux JL, Caro G, Rojas OJ et al (2012) Bacterial cellulose produced by a new acid-resistant strain of Gluconacetobacter genus. Carbohydr Polym 89:1033–1037

    CAS  PubMed  Google Scholar 

  36. Tokoh C, Takabe K, Fujita M, Saiki H (1998) Cellulose synthesized by Acetobacter xylinum in the presence of acetyl glucomannan. Cellulose 5:249–261

    CAS  Google Scholar 

  37. Wu JM, Liu RH (2013) Cost-effective production of bacterial cellulose in static cultures using distillery wastewater. J Biosci Bioeng 115:284–290

    CAS  PubMed  Google Scholar 

  38. Barud HS, Araújo Júnior AM, Santos DB, Assuncao RMN, Meireles CS, Cerqueira DA et al (2008) Thermal behavior of cellulose acetate produced from homogeneous acetylation of bacterial cellulose. Thermochim Acta 471:61–69

    CAS  Google Scholar 

  39. Mohammadkazemi F, Azin M, Ashori A (2015) Production of bacterial cellulose using different carbon sources and culture media. Carbohydr Polym 117:518–523

    CAS  PubMed  Google Scholar 

  40. Zeng ML, Laromaine A, Roig A (2014) Bacterial cellulose films: influence of bacterial strain and drying route on film properties. Cellulose 21:4455–4469

    CAS  Google Scholar 

  41. Re ZQ, Zhang DJ (2017) Effect of drying methods on structure and properties of bacterial cellulose membrane. Sci Technol Food Ind 38:91–96

    Google Scholar 

Download references

Acknowledgements

Jiuhai Legendary Wine Co., Ltd is acknowledged for the financial support of the Brewing Science and Technology Cooperation Project (17A0331-SCU) for this study. The authors would like to acknowledge the contributions of the Analytical & Testing Center of Sichuan University.

Author information

Authors and Affiliations

  1. Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, China

    Fuqiang He, Huimin Yang, Lilan Zeng, Hong Hu & Cheng Hu

Authors
  1. Fuqiang He
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Huimin Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lilan Zeng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hong Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Cheng Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Cheng Hu.

Ethics declarations

Conflict of interest

The authors report no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

He, F., Yang, H., Zeng, L. et al. Production and characterization of bacterial cellulose obtained by Gluconacetobacter xylinus utilizing the by-products from Baijiu production. Bioprocess Biosyst Eng 43, 927–936 (2020). https://doi.org/10.1007/s00449-020-02289-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00449-020-02289-6

Keywords

Search

Navigation