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The cell yields and biological characteristics of stromal/stem cells from lipoaspirate with different digestion loading ratio

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Abstract

Effective harvesting procedure for adipose tissue is demanded by the affordable Good Manufacturing Practice-Compliant Production of clinical-grade adipose tissue-derived stem cells (hADSCs). Enzymatic digestion using collagenase is the most reliable method of adipose tissue-derived stem cells (hADSCs) isolation, while the optimized loading volume ratios of digestion to container during the shaking process of adipose tissue and collagenase mixture are still lacking. This study was conducted to determine the optimized loading volume ratio (mixture to container) for enzymatic digestion of Stromal/Stem Cells from lipoaspirate. Lipoaspirates were obtained from twelve women immediately after liposuction. Then tissue from each patient was divided into four groups according to different loading volume ratios in 50 ml centrifugal tube: 0.2 group, 0.4 group, 0.6 group, 0.8 group. Stromal vascular fractions (SVF) were obtained from each group, then total cell counts, viability and viable cell count were performed. hADSCs were harvested at passage (P) 2, whose morphologies, immunophenotypes, proliferation, and tri-differentiation abilities were compared. 0.4 loading volume ratio provided the highest cell yield, favorable viability and viable cell yield. The proliferation and triple differentiation ability of hADSCs obtained by 0.4 group was not inferior to that of other groups. Therefore, 0.4 may be the optimal loading volume ratio for hADSCs isolation from lipoaspirate by enzymatic digestion in current setting.

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Funding

This work was supported by CAMS Innovation Fund for Medical Sciences (CIFMS) (2017-I2M-3-006).

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Authors and Affiliations

  1. Breast Plastic and Reconstructive Surgery Center of Plastic Surgery Hospital, Chinese Academy of Medical Science and Peking Union Medical College, 33 Ba-Da-Chu Rd, Shijingshan Dist, Beijing, 100144, People’s Republic of China

    Zifei Li, Dali Mu, Chunjun Liu, Minqiang Xin, Su Fu, Shangshan Li, Jun Qi & Jie Luan

  2. Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union of Medical College, 33 Ba-Da-Chu Rd, Shijingshan Dist, Beijing, 100144, People’s Republic of China

    Qian Wang

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  1. Zifei Li
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  2. Dali Mu
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Correspondence to Qian Wang or Jie Luan.

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All human tissue collection was approved by the Ethical Committee of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union of Medical College and was carried out in accordance with the approved guidelines.

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Li, Z., Mu, D., Liu, C. et al. The cell yields and biological characteristics of stromal/stem cells from lipoaspirate with different digestion loading ratio. Cytotechnology 72, 203–215 (2020). https://doi.org/10.1007/s10616-020-00369-9

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