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Compatible solutes adaptive alterations in Arthrobacter simplex during exposure to ethanol, and the effect of trehalose on the stress resistance and biotransformation performance

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Abstract

Ethanol-tolerant Arthrobacter simplex is desirable since ethanol facilitates hydrophobic substrates dissolution on an industrial scale. Herein, alterations in compatible solutes were investigated under ethanol stress. The results showed that the amount of trehalose and glycerol increased while that of glutamate and proline decreased. The trehalose protectant role was verified and its concentration was positively related to the degree of cell tolerance. otsA, otsB and treS, three trehalose biosynthesis genes in A. simplex, also enhanced Escherichia coli stress tolerance, but the increased tolerance was dependent on the type and level of the stress. A. simplex strains accumulating trehalose showed a higher productivity in systems containing more ethanol and substrate because of better viability. The underlying mechanisms of trehalose were involved in better cell integrity, higher membrane stability, stronger reactive oxygen species scavenging capacity and higher energy level. Therefore, trehalose was a general protectant and the upregulation of its biosynthesis by genetic modification enhanced cell stress tolerance, consequently promoted productivity.

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Acknowledgements

This work was financially supported by Natural Science Foundation of China (nos. 21978220), the Natural Science Foundation of Tianjin (nos. 18JCZDJC32500), Tianjin Technical Expert Project (nos. 19JCTPJC50800), the Open Fund of Ministry of Education Key Laboratory of Molecular Microbiology and Technology, Nankai University, Tianjin Municipal Science and Technology Commission (17PTGCCX00190), and 2019 Tianjin innovation and entrepreneurship training program for college students (national grade) (201910057051).

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  1. Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science and Technology), Ministry of Education, Tianjin Key Lab of Industrial Microbiology, Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science and Technology, 89 PO Box, No 29, St No13 Tianjin Economic-Technological Development Area (TEDA), Tianjin, 300457, People’s Republic of China

    Hong-jin Cheng, Ya-hua Sun, Han-wen Chang, Fang-fang Cui, Hai-jie Xue, Yan-bing Shen, Min Wang & Jian-mei Luo

  2. Ministry of Education Key Laboratory of Molecular Microbiology and Technology, Nankai University, Tianjin, 300071, People’s Republic of China

    Jian-mei Luo

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  1. Hong-jin Cheng
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Cheng, Hj., Sun, Yh., Chang, Hw. et al. Compatible solutes adaptive alterations in Arthrobacter simplex during exposure to ethanol, and the effect of trehalose on the stress resistance and biotransformation performance. Bioprocess Biosyst Eng 43, 895–908 (2020). https://doi.org/10.1007/s00449-020-02286-9

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