Abstract
Donor cornea contamination is one of the major risks for corneal transplants. The use of antibiotics in storage media remains as one of the most important security measurements to minimize the contamination risk in corneal preservation. Since antibiotic resistance among microorganisms have been rising gradually, it is important to gain knowledge about the antimicrobial susceptibility pattern for choosing the most suitable antimicrobial agents. Thus, we evaluated the in vitro susceptibility of microorganisms isolated in donor corneas processed at the Center for Blood Transfusion, Tissues and Cells (Córdoba, Spain) during 4 years in order to evaluate the efficiency, and to promote changes for further antibiotics use. Our results show the high rate of resistance to gentamicin, an antibiotic used in corneal preservation media such as Optisol GS and Eusol-C. Conversely, all the analyzed microorganisms were sensitive to vancomycin. This suggests the possibility of replacing gentamicin with another more effective antibacterial agent such as vancomycin.
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References
Armitage WJ (2011) Preservation of human cornea. Transfus Med Hemother 38:143–147
Brothers KM, Shanks RMQ, Hurlberts S et al (2017) Association between fungal contamination and eye bank-prepared endothelial keratoplasty tissue: temperature-dependent risk factors and antifungal supplementation of Optisol-genatmicion and streptomycin. JAMKA Ophthalmol 132:832–837
Cantón R, Pérez Vázquez M, Oliver A et al (2000) Evaluation of the Wider system, a new computer assisted image processing device for bacterial identification and susceptibility testing. J Clin Microbiol 38:1339–1346
Cantón R, Alós JI, Baquero F et al (2007) Recomendaciones para la selección de antimicrobianos en el estudio de la sensibilidad in vitro con sistemas automáticos y semiautomáticos. Enferm Infecc Microbiol Clin 25:394–400
Chen JY, Jones MN, Srinivasan S et al (2015) Endophthalmitis after penetrating keratoplasty. Ophthalmology 122:25–30
Crawford AZ, Krishnan TK, Ormonde SE et al (2018) Corneal transplantation in New Zeland 2000 to 2009. Cornea 37:290–295
Du DT, Wagoner A, Barone SB et al (2014) Incidence of endophthalmitis after corneal transplant or cataract surgery in a medicare population. Ophthalmology 121:290–298
Edelstein SL, DeMatteo J, Stoeger CG et al (2014) Report of the Eye Bank Association of America medical review subcommittee on adverse reactions reported from 2007 to 2014. Cornea 35:917–926
Espandar L, Carlson AN (2013) Lamellar keratoplasty: a literature review. J Ophtalmol 2013:894319
Fontana L, Errani PG, Zerbinati A, Musacchi Y, Di Pede B, Tassinari G (2007) Frequency of positive donor rim cultures after penetrating keratoplasty using hypothermic and organ-cultured donor corneas. Cornea 26:552–556
Gain P, Jullienne R, He Z et al (2016) Global survey of corneal transplantation and eye banking. JAMA Ophthalmol 134:167–173
Gopinathan U, Agrawal V, Sharma S et al (1994) Donor corneoscleral rim contamination by gentamicin-resistant organisms. Indian J Ophtalmol. 42:71–74
Gruenert AK, Rosenbaum K, Geerling G, Fuchsluger TA (2017) The influence of donor factor on corneal organ culture contamination. Acta Ophthalmol. https://doi.org/10.1111/aos.13402
Jonson AP, Livermores DM, Tillotson GS (2001) Antimicrobial susceptibility of gram-positive bacteria: What’s current, what’s anticipated. J Hosp Infect 49(Supplement A):S3–S11
Kryczka T, Szaflik JP, Szaflik J, Midelfart A (2013) Influence of donor age, post-mortem time and cold storage on metabolic profile of human cornea. Acta Ophtalmol 91:83–87
Langer C, Eichhorst A, Duncker GIW et al (2002) Alternative explantation techniques for corneal donation. Transplant Proc 34:2336–2337
Laubichler P, Arend N, Vounotrypidis E et al (2016) Comparing different decontamination procedures in harvesting human donor cornea. Curr Eye Res 41:1173–1177
Linke SJ, Fricke OH, Eddy MT et al (2013) Risk factors for donor cornea contamination: retrospective analysis of 4546 procured corneas in a single eye bank. Cornea 32:141–148
Low DE, Keller N, Bath A, Jones RN (2001) Clinical prevalence, antimicrobial susceptibility, and geographic resistance patterns of enterococci: results from SENTRY antimicrobial surveillance program, 1997–199. Clin Infect Dis 32(Suppl 2):S133–S145
Ormerod LD, Heseltine PNR, Allenso E et al (1989) Gentamicin resistant Pseudomonas infection. Rationale for a redefinition of ophthalmic antimicrobial sensitivities. Cornea 8:195–199
Pardes GJ, Gallager MA (1982) Microbial contamination of donor eyes: a retrospective study. Arch Ophthalmol 100:1611–1613
Rehany U, Balut G, Lefler E, Rumelt S (2004) The prevalence and risk factors for donor corneal button contamination and its association with ocular infection after transplantation. Cornea 23:649–654
Röck D, Wude J, Bartz-Schmidt KU, Yoeruek E, Thaler S, Röck T (2017) Factors influencing the contamination rate of human organ-cultured corneas. Acta Ophthalmol. https://doi.org/10.1111/aos.13375
Tan DT, Dart JK, Holland EJ, Kinoshita S (2012) Corneal transplantation. Lancet 379:1749–1761
Tran KD, Adrich BT, Tóthová JD et al (2019) Efficacy and safety of various amphotericin B concentrations on Candida albicans in cold storage conditions. Cornea. https://doi.org/10.1097/ICO.0000000000002019
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All authors were involved in the final approval of the article submitted and are in agreement with all the content. Marta Villalba, Maria Jose Linares-Sicilia and Rafael Villalba participated in the design and methodology. Marta Villalba collected data for the study. Miguel González-Andrades, Marcelina Eisman, Javier Giménez Almenara y Manuel Arias contributed to the revision and expert opinion.
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Marta Villalba, Maria José Linares-Sicilia, Miguel González-Andrades, Marcelina Eisman, Javier Giménez Almenara, Manuel Arias and Rafael Villalba declare that have no conflict of interest.
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Villalba, M., Linares-Sicilia, M.J., González-Andrades, M. et al. In vitro susceptibility of microorganisms isolated from cold stored corneas: increased gentamicin-resistance in cornea banking. Cell Tissue Bank 21, 159–165 (2020). https://doi.org/10.1007/s10561-020-09811-w
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DOI: https://doi.org/10.1007/s10561-020-09811-w